Upregulation of Mrps18a in breast cancer identified by selecting phage antibody libraries on breast tissue sections

Karen Marie Juul Sørensen; Theresa Meldgaard; Connie Jenning Melchjorsen; Agla J Fridriksdottir; Henrik Pedersen; Ole William Petersen; Peter Kristensen

doi:10.1186/s12885-016-2987-5

Upregulation of Mrps18a in breast cancer identified by selecting phage antibody libraries on breast tissue sections

Karen Marie Juul Sørensen, Theresa Meldgaard, Connie Jenning Melchjorsen, Agla J Fridriksdottir, Henrik Pedersen, Ole William Petersen, Peter Kristensen

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

15 Citationer (Scopus)

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Abstract

Background: One of the hallmarks of cancer is an altered energy metabolism, and here, mitochondria play a central role. Previous studies have indicated that some mitochondrial ribosomal proteins change their expression patterns upon transformation. Method: In this study, we have used the selection of recombinant antibody libraries displayed on the surface of filamentous bacteriophage as a proteomics discovery tool for the identification of breast cancer biomarkers. A small subpopulation of breast cells expressing both cytokeratin 19 and cytokeratin 14 was targeted using a novel selection procedure. Results: We identified the mitochondrial ribosomal protein s18a (Mrps18a) as a protein which is upregulated in breast cancer. However, Mrps18a was not homogeneously upregulated in all cancer cells, suggesting the existence of sub-populations within the tumor. The upregulation was not confined to cytokeratin 19 and cytokeratin 14 double positive cells. Conclusion: This study illustrates how phage display can be applied towards the discovery of proteins which exhibit changes in their expression patterns. We identified the mitochondrial protein Mrps18a as being upregulated in human breast cancer cells compared to normal breast cells.

Originalsprog	Engelsk
Artikelnummer	19
Tidsskrift	BMC Cancer
Vol/bind	17
Nummer	1
Sider (fra-til)	19
Antal sider	11
ISSN	1471-2407
DOI	https://doi.org/10.1186/s12885-016-2987-5
Status	Udgivet - 5 jan. 2017

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10.1186/s12885-016-2987-5Licens: CC0

s12885-016-2987-5Forlagets udgivne version, 4,06 MBLicens: CC0

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abstract = "Background: One of the hallmarks of cancer is an altered energy metabolism, and here, mitochondria play a central role. Previous studies have indicated that some mitochondrial ribosomal proteins change their expression patterns upon transformation. Method: In this study, we have used the selection of recombinant antibody libraries displayed on the surface of filamentous bacteriophage as a proteomics discovery tool for the identification of breast cancer biomarkers. A small subpopulation of breast cells expressing both cytokeratin 19 and cytokeratin 14 was targeted using a novel selection procedure. Results: We identified the mitochondrial ribosomal protein s18a (Mrps18a) as a protein which is upregulated in breast cancer. However, Mrps18a was not homogeneously upregulated in all cancer cells, suggesting the existence of sub-populations within the tumor. The upregulation was not confined to cytokeratin 19 and cytokeratin 14 double positive cells. Conclusion: This study illustrates how phage display can be applied towards the discovery of proteins which exhibit changes in their expression patterns. We identified the mitochondrial protein Mrps18a as being upregulated in human breast cancer cells compared to normal breast cells.",

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Upregulation of Mrps18a in breast cancer identified by selecting phage antibody libraries on breast tissue sections. / Sørensen, Karen Marie Juul; Meldgaard, Theresa; Melchjorsen, Connie Jenning et al.
I: BMC Cancer, Bind 17, Nr. 1, 19, 05.01.2017, s. 19.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Upregulation of Mrps18a in breast cancer identified by selecting phage antibody libraries on breast tissue sections

AU - Sørensen, Karen Marie Juul

AU - Meldgaard, Theresa

AU - Melchjorsen, Connie Jenning

AU - Fridriksdottir, Agla J

AU - Pedersen, Henrik

AU - Petersen, Ole William

AU - Kristensen, Peter

PY - 2017/1/5

Y1 - 2017/1/5

N2 - Background: One of the hallmarks of cancer is an altered energy metabolism, and here, mitochondria play a central role. Previous studies have indicated that some mitochondrial ribosomal proteins change their expression patterns upon transformation. Method: In this study, we have used the selection of recombinant antibody libraries displayed on the surface of filamentous bacteriophage as a proteomics discovery tool for the identification of breast cancer biomarkers. A small subpopulation of breast cells expressing both cytokeratin 19 and cytokeratin 14 was targeted using a novel selection procedure. Results: We identified the mitochondrial ribosomal protein s18a (Mrps18a) as a protein which is upregulated in breast cancer. However, Mrps18a was not homogeneously upregulated in all cancer cells, suggesting the existence of sub-populations within the tumor. The upregulation was not confined to cytokeratin 19 and cytokeratin 14 double positive cells. Conclusion: This study illustrates how phage display can be applied towards the discovery of proteins which exhibit changes in their expression patterns. We identified the mitochondrial protein Mrps18a as being upregulated in human breast cancer cells compared to normal breast cells.

AB - Background: One of the hallmarks of cancer is an altered energy metabolism, and here, mitochondria play a central role. Previous studies have indicated that some mitochondrial ribosomal proteins change their expression patterns upon transformation. Method: In this study, we have used the selection of recombinant antibody libraries displayed on the surface of filamentous bacteriophage as a proteomics discovery tool for the identification of breast cancer biomarkers. A small subpopulation of breast cells expressing both cytokeratin 19 and cytokeratin 14 was targeted using a novel selection procedure. Results: We identified the mitochondrial ribosomal protein s18a (Mrps18a) as a protein which is upregulated in breast cancer. However, Mrps18a was not homogeneously upregulated in all cancer cells, suggesting the existence of sub-populations within the tumor. The upregulation was not confined to cytokeratin 19 and cytokeratin 14 double positive cells. Conclusion: This study illustrates how phage display can be applied towards the discovery of proteins which exhibit changes in their expression patterns. We identified the mitochondrial protein Mrps18a as being upregulated in human breast cancer cells compared to normal breast cells.

KW - Breast cancer

KW - Domain antibodies

KW - Mitochondrial ribosomal protein s18a

KW - Phage display

KW - Shadow stick selection

U2 - 10.1186/s12885-016-2987-5

DO - 10.1186/s12885-016-2987-5

M3 - Journal article

C2 - 28056857

SN - 1471-2407

VL - 17

SP - 19

JO - BMC Cancer

JF - BMC Cancer

IS - 1

M1 - 19

ER -

Upregulation of Mrps18a in breast cancer identified by selecting phage antibody libraries on breast tissue sections

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