Unraveling plant-microbe symbioses using single-cell and spatial transcriptomics

Karen Serrano; Francesca Tedeschi; Stig U Andersen; Henrik V Scheller

doi:10.1016/j.tplants.2024.06.008

Unraveling plant-microbe symbioses using single-cell and spatial transcriptomics

Karen Serrano, Francesca Tedeschi, Stig U Andersen, Henrik V Scheller

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Review › Forskning › peer review

3 Citationer (Scopus)

Abstract

Plant-microbe symbioses require intense interaction and genetic coordination to successfully establish in specific cell types of the host and symbiont. Traditional RNA-seq methodologies lack the cellular resolution to fully capture these complexities, but single-cell and spatial transcriptomics (ST) are now allowing scientists to probe symbiotic interactions at an unprecedented level of detail. Here, we discuss the advantages that novel spatial and single-cell transcriptomic technologies provide in studying plant-microbe endosymbioses and highlight key recent studies. Finally, we consider the remaining limitations of applying these approaches to symbiosis research, which are mainly related to the simultaneous capture of both plant and microbial transcripts within the same cells.

Originalsprog	Engelsk
Tidsskrift	Trends in Plant Science
ISSN	1360-1385
DOI	https://doi.org/10.1016/j.tplants.2024.06.008
Status	E-pub ahead of print - 10 jul. 2024

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title = "Unraveling plant-microbe symbioses using single-cell and spatial transcriptomics",

abstract = "Plant-microbe symbioses require intense interaction and genetic coordination to successfully establish in specific cell types of the host and symbiont. Traditional RNA-seq methodologies lack the cellular resolution to fully capture these complexities, but single-cell and spatial transcriptomics (ST) are now allowing scientists to probe symbiotic interactions at an unprecedented level of detail. Here, we discuss the advantages that novel spatial and single-cell transcriptomic technologies provide in studying plant-microbe endosymbioses and highlight key recent studies. Finally, we consider the remaining limitations of applying these approaches to symbiosis research, which are mainly related to the simultaneous capture of both plant and microbial transcripts within the same cells.",

keywords = "high-throughput sequencing, mycorrhizae, rhizobia, single-cell RNA-seq, spatial transcriptomics, symbiosis",

author = "Karen Serrano and Francesca Tedeschi and Andersen, {Stig U} and Scheller, {Henrik V}",

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AU - Serrano, Karen

AU - Tedeschi, Francesca

AU - Andersen, Stig U

AU - Scheller, Henrik V

PY - 2024/7/10

Y1 - 2024/7/10

N2 - Plant-microbe symbioses require intense interaction and genetic coordination to successfully establish in specific cell types of the host and symbiont. Traditional RNA-seq methodologies lack the cellular resolution to fully capture these complexities, but single-cell and spatial transcriptomics (ST) are now allowing scientists to probe symbiotic interactions at an unprecedented level of detail. Here, we discuss the advantages that novel spatial and single-cell transcriptomic technologies provide in studying plant-microbe endosymbioses and highlight key recent studies. Finally, we consider the remaining limitations of applying these approaches to symbiosis research, which are mainly related to the simultaneous capture of both plant and microbial transcripts within the same cells.

AB - Plant-microbe symbioses require intense interaction and genetic coordination to successfully establish in specific cell types of the host and symbiont. Traditional RNA-seq methodologies lack the cellular resolution to fully capture these complexities, but single-cell and spatial transcriptomics (ST) are now allowing scientists to probe symbiotic interactions at an unprecedented level of detail. Here, we discuss the advantages that novel spatial and single-cell transcriptomic technologies provide in studying plant-microbe endosymbioses and highlight key recent studies. Finally, we consider the remaining limitations of applying these approaches to symbiosis research, which are mainly related to the simultaneous capture of both plant and microbial transcripts within the same cells.

KW - high-throughput sequencing

KW - mycorrhizae

KW - rhizobia

KW - single-cell RNA-seq

KW - spatial transcriptomics

KW - symbiosis

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DO - 10.1016/j.tplants.2024.06.008

M3 - Review

C2 - 38991926

SN - 1360-1385

JO - Trends in Plant Science

JF - Trends in Plant Science

ER -

Unraveling plant-microbe symbioses using single-cell and spatial transcriptomics

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