Ultra-Small Graphene Oxide Functionalized with Polyethylenimine (PEI) for Very Efficient Gene Delivery in Cell and Zebrafish Embryos

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Ultra-Small Graphene Oxide Functionalized with Polyethylenimine (PEI) for Very Efficient Gene Delivery in Cell and Zebrafish Embryos. / Zhou, Xiang; Laroche, Fabrice Jean Francois; Lamers, Gerda E M; Torraca, Vincenzo; Voskamp, Patrick; Lu, Tao; Chu, Fuqiang; Spaink, Herman P; Abrahams, Jan Pieter; Liu, Zunfeng.

I: Nano Research, Bind 5, Nr. 10, 2012, s. 703-709.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Harvard

Zhou, X, Laroche, FJF, Lamers, GEM, Torraca, V, Voskamp, P, Lu, T, Chu, F, Spaink, HP, Abrahams, JP & Liu, Z 2012, 'Ultra-Small Graphene Oxide Functionalized with Polyethylenimine (PEI) for Very Efficient Gene Delivery in Cell and Zebrafish Embryos', Nano Research, bind 5, nr. 10, s. 703-709. https://doi.org/10.1007/s12274-012-0254-x

APA

Zhou, X., Laroche, F. J. F., Lamers, G. E. M., Torraca, V., Voskamp, P., Lu, T., Chu, F., Spaink, H. P., Abrahams, J. P., & Liu, Z. (2012). Ultra-Small Graphene Oxide Functionalized with Polyethylenimine (PEI) for Very Efficient Gene Delivery in Cell and Zebrafish Embryos. Nano Research, 5(10), 703-709. https://doi.org/10.1007/s12274-012-0254-x

Author

Zhou, Xiang ; Laroche, Fabrice Jean Francois ; Lamers, Gerda E M ; Torraca, Vincenzo ; Voskamp, Patrick ; Lu, Tao ; Chu, Fuqiang ; Spaink, Herman P ; Abrahams, Jan Pieter ; Liu, Zunfeng. / Ultra-Small Graphene Oxide Functionalized with Polyethylenimine (PEI) for Very Efficient Gene Delivery in Cell and Zebrafish Embryos. I: Nano Research. 2012 ; Bind 5, Nr. 10. s. 703-709.

Bibtex

@article{2ddc34fbd4dc43efb58581e2d7b72a10,

title = "Ultra-Small Graphene Oxide Functionalized with Polyethylenimine (PEI) for Very Efficient Gene Delivery in Cell and Zebrafish Embryos",

abstract = "Efficient DNA delivery is essential for introducing new genes into living cells. However, effective virus-based systems carry risks and efficient synthetic systems that are non-toxic remain to be discovered. The bottle-neck in synthetic systems is cytotoxicity, caused by the high concentration of DNA-condensing compounds required for efficient uptake of DNA. Here we report a polyethyleneimine (PEI) grafted ultra-small graphene oxide (PEI-g-USGO) for transfection. By removing the free PEI and ensuring a high PEI density on small sized graphene, we obtained very high transfection efficiencies combined with very low cytotoxicity. Plasmid DNA could be transfected into mammalian cell lines with up to 95% efficiency and 90% viability. Transfection in zebrafish embryos was 90%, with high viability, compared to efficiencies of 30% or lower for established transfection technologies. This result suggests a novel approach to the design of synthetic gene delivery vehicles for research and therapy. ",

author = "Xiang Zhou and Laroche, {Fabrice Jean Francois} and Lamers, {Gerda E M} and Vincenzo Torraca and Patrick Voskamp and Tao Lu and Fuqiang Chu and Spaink, {Herman P} and Abrahams, {Jan Pieter} and Zunfeng Liu",

year = "2012",

doi = "10.1007/s12274-012-0254-x",

language = "English",

volume = "5",

pages = "703--709",

journal = "Nano Research",

issn = "1998-0124",

publisher = "Tsinghua University press,",

number = "10",

}

RIS

TY - JOUR

T1 - Ultra-Small Graphene Oxide Functionalized with Polyethylenimine (PEI) for Very Efficient Gene Delivery in Cell and Zebrafish Embryos

AU - Zhou, Xiang

AU - Laroche, Fabrice Jean Francois

AU - Lamers, Gerda E M

AU - Torraca, Vincenzo

AU - Voskamp, Patrick

AU - Lu, Tao

AU - Chu, Fuqiang

AU - Spaink, Herman P

AU - Abrahams, Jan Pieter

AU - Liu, Zunfeng

PY - 2012

Y1 - 2012

N2 - Efficient DNA delivery is essential for introducing new genes into living cells. However, effective virus-based systems carry risks and efficient synthetic systems that are non-toxic remain to be discovered. The bottle-neck in synthetic systems is cytotoxicity, caused by the high concentration of DNA-condensing compounds required for efficient uptake of DNA. Here we report a polyethyleneimine (PEI) grafted ultra-small graphene oxide (PEI-g-USGO) for transfection. By removing the free PEI and ensuring a high PEI density on small sized graphene, we obtained very high transfection efficiencies combined with very low cytotoxicity. Plasmid DNA could be transfected into mammalian cell lines with up to 95% efficiency and 90% viability. Transfection in zebrafish embryos was 90%, with high viability, compared to efficiencies of 30% or lower for established transfection technologies. This result suggests a novel approach to the design of synthetic gene delivery vehicles for research and therapy.

AB - Efficient DNA delivery is essential for introducing new genes into living cells. However, effective virus-based systems carry risks and efficient synthetic systems that are non-toxic remain to be discovered. The bottle-neck in synthetic systems is cytotoxicity, caused by the high concentration of DNA-condensing compounds required for efficient uptake of DNA. Here we report a polyethyleneimine (PEI) grafted ultra-small graphene oxide (PEI-g-USGO) for transfection. By removing the free PEI and ensuring a high PEI density on small sized graphene, we obtained very high transfection efficiencies combined with very low cytotoxicity. Plasmid DNA could be transfected into mammalian cell lines with up to 95% efficiency and 90% viability. Transfection in zebrafish embryos was 90%, with high viability, compared to efficiencies of 30% or lower for established transfection technologies. This result suggests a novel approach to the design of synthetic gene delivery vehicles for research and therapy.

U2 - 10.1007/s12274-012-0254-x

DO - 10.1007/s12274-012-0254-x

M3 - Journal article

VL - 5

SP - 703

EP - 709

JO - Nano Research

JF - Nano Research

SN - 1998-0124

IS - 10

ER -