Ultra-Small Graphene Oxide Functionalized with Polyethylenimine (PEI) for Very Efficient Gene Delivery in Cell and Zebrafish Embryos

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  • Xiang Zhou, Biophysical Structural Chemistry, Cell Observatory, Leiden Institute of Chemistry, Holland
  • Fabrice Jean Francois Laroche, Danmark
  • Gerda E M Lamers, The Institute of Biology Leiden, Gorlaeus Laboratoria, Leiden University, Holland
  • Vincenzo Torraca, The Institute of Biology Leiden, Gorlaeus Laboratoria, Leiden University, Holland
  • Patrick Voskamp, Biophysical Structural Chemistry, Cell Observatory, Leiden Institute of Chemistry, Holland
  • Tao Lu, State Key Laboratory of Natural Medicines, Department of Organic Chemistry, China Pharmaceutical University, Kina
  • Fuqiang Chu, School of pharmaceutical engineering and life science, Changzhou University, Kina
  • Herman P Spaink, The Institute of Biology Leiden, Gorlaeus Laboratoria, Leiden University, Holland
  • Jan Pieter Abrahams, Biophysical Structural Chemistry, Cell Observatory, Leiden Institute of Chemistry, Holland
  • Zunfeng Liu, Biophysical Structural Chemistry, Cell Observatory, Leiden Institute of Chemistry, Holland
Efficient DNA delivery is essential for introducing new genes into living cells. However, effective virus-based systems carry risks and efficient synthetic systems that are non-toxic remain to be discovered. The bottle-neck in synthetic systems is cytotoxicity, caused by the high concentration of DNA-condensing compounds required for efficient uptake of DNA. Here we report a polyethyleneimine (PEI) grafted ultra-small graphene oxide (PEI-g-USGO) for transfection. By removing the free PEI and ensuring a high PEI density on small sized graphene, we obtained very high transfection efficiencies combined with very low cytotoxicity. Plasmid DNA could be transfected into mammalian cell lines with up to 95% efficiency and 90% viability. Transfection in zebrafish embryos was 90%, with high viability, compared to efficiencies of 30% or lower for established transfection technologies. This result suggests a novel approach to the design of synthetic gene delivery vehicles for research and therapy.
OriginalsprogEngelsk
TidsskriftNano Research
Vol/bind5
Nummer10
Sider (fra-til)703-709
Antal sider7
ISSN1998-0124
DOI
StatusUdgivet - 2012

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