Ultrafiltration of Rapeseed Protein Concentrate: Effect of Pectinase Treatment on Membrane Fouling

TY - JOUR

T1 - Ultrafiltration of Rapeseed Protein Concentrate: Effect of Pectinase Treatment on Membrane Fouling

AU - Alpiger, Simone Bleibach

AU - Solet, Chloé

AU - Dang, Thi Tem

AU - Corredig, Milena

PY - 2024/8

Y1 - 2024/8

N2 - Membrane filtration technologies have shown great potential as a gentle and effective method for concentrating and fractionating proteins for food applications. However, the application of this technology to plant-derived protein streams is in its infancy. In this study, an aqueous rapeseed protein concentrate was obtained with wet milling, and its performance during ultrafiltration with two distinct molecular weight cut-offs (10 and 100 kDa) was tested. All rapeseed proteins were retained during filtration. The addition of pectinase during extraction prior to filtration caused important structural modifications to the extract, resulting in increased permeate fluxes, increased carbohydrate permeation and a reduction in irreversible fouling. Lager pore sizes led to more pronounced fouling. FTIR analysis of the spent membranes showed that proteins and lipids are causing irreversible fouling.

AB - Membrane filtration technologies have shown great potential as a gentle and effective method for concentrating and fractionating proteins for food applications. However, the application of this technology to plant-derived protein streams is in its infancy. In this study, an aqueous rapeseed protein concentrate was obtained with wet milling, and its performance during ultrafiltration with two distinct molecular weight cut-offs (10 and 100 kDa) was tested. All rapeseed proteins were retained during filtration. The addition of pectinase during extraction prior to filtration caused important structural modifications to the extract, resulting in increased permeate fluxes, increased carbohydrate permeation and a reduction in irreversible fouling. Lager pore sizes led to more pronounced fouling. FTIR analysis of the spent membranes showed that proteins and lipids are causing irreversible fouling.

KW - Membranfiltrering

KW - Rapsprotein

KW - irreversible fouling

KW - pectinase

KW - rapeseed protein

KW - reversible fouling

UR - http://www.scopus.com/inward/record.url?scp=85200728456&partnerID=8YFLogxK

U2 - 10.3390/foods13152423

DO - 10.3390/foods13152423

M3 - Journal article

C2 - 39123614

SN - 2304-8158

VL - 13

JO - Foods

JF - Foods

IS - 15

M1 - 2423

ER -