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Transient Complexity of E. coli Lipidome Is Explained by Fatty Acyl Synthesis and Cyclopropanation
Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review
DOI
- https://doi.org/10.3390/metabo12090784
Forlagets udgivne version
- Nikolay V. Berezhnoy, Nanyang Technological University, National University of Singapore ,
- Amaury Cazenave-Gassiot, National University of Singapore ,
- Liang Gao, National University of Singapore ,
- Juat Chin Foo, National University of Singapore ,
- Shanshan Ji, National University of Singapore ,
- Viduthalai Rasheedkhan Regina, Nanyang Technological University ,
- Pui Khee Peggy Yap, Nanyang Technological University ,
- Markus R. Wenk, National University of Singapore ,
- Staffan Kjelleberg, Nanyang Technological University ,
- Thomas William Seviour
- Jamie Hinks, Nanyang Technological University
In the case of many bacteria, such as Escherichia coli, the composition of lipid molecules, termed the lipidome, temporally adapts to different environmental conditions and thus modifies membrane properties to permit growth and survival. Details of the relationship between the environment and lipidome composition are lacking, particularly for growing cultures under either favourable or under stress conditions. Here, we highlight compositional lipidome changes by describing the dynamics of molecular species throughout culture-growth phases. We show a steady cyclopropanation of fatty acyl chains, which acts as a driver for lipid diversity. There is a bias for the cyclopropanation of shorter fatty acyl chains (FA 16:1) over longer ones (FA 18:1), which likely reflects a thermodynamic phenomenon. Additionally, we observe a nearly two-fold increase in saturated fatty acyl chains in response to the presence of ampicillin and chloramphenicol, with consequences for membrane fluidity and elasticity, and ultimately bacterial stress tolerance. Our study provides the detailed quantitative lipidome composition of three E. coli strains across culture-growth phases and at the level of the fatty acyl chains and provides a general reference for phospholipid composition changes in response to perturbations. Thus, lipidome diversity is largely transient and the consequence of lipid synthesis and cyclopropanation.
| Originalsprog | Engelsk |
|---|---|
| Artikelnummer | 784 |
| Tidsskrift | Metabolites |
| Vol/bind | 12 |
| Nummer | 9 |
| Antal sider | 18 |
| ISSN | 2218-1989 |
| DOI | |
| Status | Udgivet - sep. 2022 |
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