Transformation and regeneration of the legume Lotus corniculatus: A system for molecular studies of symbiotic nitrogen fixation

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DOI

  • Annik Petit, Université Paris-Sud 11
  • ,
  • Jens Stougaard
  • Astrid Kühle, Department of Molecular Biology and Plant Physiology
  • ,
  • Kjeld A. Marcker, Department of Molecular Biology and Plant Physiology
  • ,
  • Jacques Tempé, Université Paris-Sud 11

A procedure for transformation and regeneration of the legume species Lotus corniculatus (Bird's-foot trefoil) has been developed. The Agrobacterium rhizogenes 15834 and 8196 strains were used to transform plant cells in wound site infections and transformed roots were propagated in vitro. Transformation was monitored by hybridization with pRi T-DNA sequences and by detection of agropine and mannopine. Transformation frequencies of up to 90% were obtained. Shoots spontaneously formed on hairy root cultures were excised, rooted and inoculated with Rhizobium. Root nodules formed on transformed plants had nitrogenase activities comparable to untransformed nodules. Transcript levels from the nodule-specific leghemoglobin genes and the constitutive ubiquitin genes were similar in transformed and untransformed root nodules. Transformed plants responded to R. loti and Bradyrhizobium sp. (Lotus) strains with phenotypes identical to phenotypes for untransformed plants.

OriginalsprogEngelsk
TidsskriftMGG Molecular & General Genetics
Vol/bind207
Nummer2-3
Sider (fra-til)245-250
Antal sider6
ISSN0026-3925
DOI
StatusUdgivet - maj 1987
Eksternt udgivetJa

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