Transcriptome dynamics during proliferation and differentiation of porcine primary satellite cells

TY - JOUR

T1 - Transcriptome dynamics during proliferation and differentiation of porcine primary satellite cells

AU - Jensen, Jeanette H.

AU - Madsen, Lone Bruhn

AU - Panitz, Frank

AU - Hornshøj, Henrik

AU - Nielsen, Rasmus Ory

AU - Bendixen, Christian

AU - Oksbjerg, Niels

AU - Thomsen, Bo

PY - 2017/6/1

Y1 - 2017/6/1

N2 - During myogenesis the satellite cells undergo a series of highly ordered events that include division and proliferation of mononucleated myoblasts, irreversible cell cycle withdrawal, elongation of myoblasts and finally cell-cell fusion, leading to the formation of multinucleated myotubes. These cellular processes are strictly regulated in a spatiotemporally manner through a coordinated patterns of gene expression governed by specific transcriptional activators and networks of signal transduction. Here we analyzed the genome-wide changes in gene transcription occurring during growth and differentiation of porcine primary myoblast cells. Analysis for differential expression revealed 1656 gene transcripts with significant changes in expression levels during myogenesis. These analyses expand our knowledge of the temporal expression patterns and provide an insight into known and unknown genes and pathways that are transcriptionally regulated and, therefore, likely to be critically implicated in myogenesis. Intriguingly, a remarkable number of the differentially expressed genes encode proteins engaged in histone modification and remodeling of nucleosome structure, emphasizing that extensive changes in chromatin structure are required for regulated gene transcription in the transition from proliferating myoblasts to terminally differentiated myotubes.

AB - During myogenesis the satellite cells undergo a series of highly ordered events that include division and proliferation of mononucleated myoblasts, irreversible cell cycle withdrawal, elongation of myoblasts and finally cell-cell fusion, leading to the formation of multinucleated myotubes. These cellular processes are strictly regulated in a spatiotemporally manner through a coordinated patterns of gene expression governed by specific transcriptional activators and networks of signal transduction. Here we analyzed the genome-wide changes in gene transcription occurring during growth and differentiation of porcine primary myoblast cells. Analysis for differential expression revealed 1656 gene transcripts with significant changes in expression levels during myogenesis. These analyses expand our knowledge of the temporal expression patterns and provide an insight into known and unknown genes and pathways that are transcriptionally regulated and, therefore, likely to be critically implicated in myogenesis. Intriguingly, a remarkable number of the differentially expressed genes encode proteins engaged in histone modification and remodeling of nucleosome structure, emphasizing that extensive changes in chromatin structure are required for regulated gene transcription in the transition from proliferating myoblasts to terminally differentiated myotubes.

KW - Cell growth and differentiation

KW - Chromatin structure

KW - Satellite cells

KW - Transcriptome

UR - https://www.scopus.com/pages/publications/85018438795

U2 - 10.1016/j.genrep.2017.04.007

DO - 10.1016/j.genrep.2017.04.007

M3 - Journal article

SN - 2452-0144

VL - 7

SP - 169

EP - 175

JO - Gene Reports

JF - Gene Reports

ER -