Toward stable genetic engineering of human O-glycosylation in plants

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Dokumenter

DOI

  • Zhang Yang, Danmark
  • Eric Paul Bennett, Sektion 03, Danmark
  • Bodil Jørgensen, 2012 Plante- og Jordvidenskab, Danmark
  • Damian Paul Drew, 2012 Institut for Plantebiologi og Bioteknologi, Danmark
  • Emma Adhiambo Arigi, Odontologisk Institut, Danmark
  • Ulla Mandel, Sektion 03, Danmark
  • Peter Ulvskov, Planteglycobiologi, Danmark
  • Steven Bruce Levery, Afdeling III, Danmark
  • Henrik Clausen, Afdeling II, Danmark
  • Bent L Petersen, 2012 Planteglykobiologi, Danmark
Glycosylation is the most abundant and complex posttranslational modification to be considered for recombinant production of therapeutic proteins. Mucin-type (N-acetylgalactosamine [GalNAc]-type) O-glycosylation is found in eumetazoan cells but absent in plants and yeast, making these cell types an obvious choice for de novo engineering of this O-glycosylation pathway. We previously showed that transient implementation of O-glycosylation capacity in plants requires introduction of the synthesis of the donor substrate UDP-GalNAc and one or more polypeptide GalNAc-transferases for incorporating GalNAc residues into proteins. Here, we have stably engineered O-glycosylation capacity in two plant cell systems, soil-grown Arabidopsis (Arabidopsis thaliana) and tobacco (Nicotiana tabacum) Bright Yellow-2 suspension culture cells. Efficient GalNAc O-glycosylation of two stably coexpressed substrate O-glycoproteins was obtained, but a high degree of proline hydroxylation and hydroxyproline-linked arabinosides, on a mucin (MUC1)-derived substrate, was also observed. Addition of the prolyl 4-hydroxylase inhibitor 2,2-dipyridyl, however, effectively suppressed proline hydroxylation and arabinosylation of MUC1 in Bright Yellow-2 cells. In summary, stably engineered mammalian type O-glycosylation was established in transgenic plants, demonstrating that plants may serve as host cells for the production of recombinant O-glycoproteins. However, the present stable implementation further strengthens the notion that elimination of endogenous posttranslational modifications may be needed for the production of protein therapeutics.
OriginalsprogEngelsk
TidsskriftPlant Physiology
Vol/bind160
Nummer1
Sider (fra-til)450-463
Antal sider14
ISSN0032-0889
DOI
StatusUdgivet - 2012

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