Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats

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    Abstract

    Purpose Hydroxocobalamin (HOCbl) is the dominating Cbl form in food, whereas cyanocobalamin (CNCbl) is common in vitamin pills and oral supplements. This study compares single-dose absorption and distribution of oral HO[Co-57] Cbl and CN[Co-57] Cbl in Cbl-deficient and normal rats.

    Methods Male Wistar rats (7 weeks) were fed a 14-day diet with (n = 15) or without (n = 15) Cbl. We compared the uptakes of HO[Co-57] Cbl (free or bound to bovine transcobalamin) and free CN[Co-57] Cbl administered by gastric gavage (n = 5 in each diet group). Rats were sacrificed after 24 h. Blood, liver, kidney, brain, heart, spleen, intestines, skeletal muscle, 24-h urine and faeces were collected, and the content of [Co-57] Cbl was measured. Endogenous Cbl in tissues and plasma was analysed by routine methods.

    Results Mean endogenous plasma-Cbl was sevenfold lower in deficient vs. normal rats (190 vs. 1330 pmol/L, p <0.0001). Cbl depletion increased endogenous Cbl ratios (tissue/plasma = k(in)/k(out)) in all organs except for the kidney, where the ratio decreased considerably. Twenty-four-hour accumulation of labelled Cbl showed that HOCbl > CNCbl (liver) and CNCbl > HOCbl (brain, muscle and plasma).

    Conclusions The Cbl status of rats and the administered Cbl form influence 24-h Cbl accumulation in tissues and plasma.

    OriginalsprogEngelsk
    Artikelnummer4
    TidsskriftEuropean Journal of Nutrition
    Vol/bind57
    Nummer4
    Sider (fra-til)1459-1469
    Antal sider11
    ISSN1436-6207
    DOI
    StatusUdgivet - jun. 2018

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