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TiO2 nanoparticles' library toxicity (UV and non-UV exposure) – High-throughput in vivo transcriptomics reveals mechanisms

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  • Susana I.L. Gomes, University of Aveiro
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  • Carlos P. Roca
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  • Suman Pokhrel, University of Bremen, Leibniz Institute for Materials Engineering
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  • Lutz Mädler, University of Bremen, Leibniz Institute for Materials Engineering
  • ,
  • Janeck J. Scott-Fordsmand
  • Mónica J.B. Amorim, University of Aveiro

The hazards of nanomaterials/nanoparticles (NMs/NPs) are mostly assessed using individual NMs, and a more systematic approach, using many NMs, is needed to evaluate its risks in the environment. Libraries of NMs, with a range of identified different but related characters/descriptors allow the comparison of effects across many NMs. The effects of a custom designed Fe-doped TiO2 NMs library containing 11 NMs was assessed on the soil model Enchytraeus crypticus (Oligochaeta), both with and without UV (standard fluorescent) radiation. Effects were analyzed at organism (phenotypic, survival and reproduction) and gene expression level (transcriptomics, high-throughput 4x44K microarray) to understand the underlying mechanisms. A total of 48 microarrays (20 test conditions) were done plus controls (UV and non-UV). Unique mechanisms induced by TiO2 NPs exposure included the impairment in RNA processing for TiO2_10nm, or deregulated apoptosis for 2%FeTiO2_10nm. Strikingly apparent was the size dependent effects such as induction of reproductive effects via smaller TiO2 NPs (≤12 nm) - embryo interaction, while larger particles (27 nm) caused reproductive effects through different mechanisms. Also, phagocytosis was affected by 12 and 27 nm NPs, but not by ≤11 nm. The organism level study shows the integrated response, i.e. the result after a cascade of events. While uni-cell models offer key mechanistic information, we here deliver a combined biological system level (phenotype and genotype), seldom available, especially for environmental models.

StatusUdgivet - apr. 2023

Bibliografisk note

Funding Information:
This study was supported by funds of the European Commission NANOINFORMATIX ( H2020-NMBP-14-2018 , No. 814426), BIORIMA (H2020-NMBP-12-2017, GA No. 760928) and NANORIGO (H2020-NMBP-13-2018, GA No. 814530). Further support from FEDER through COMPETE Programa Operacional Factores de Competitividade (2020) and the Portuguese Science Foundation (FCT-Fundação para a Ciência e Tecnologia) within the projects BIO-chip (EXPL/AAG-MAA/0180/2013) and NM OREO (PTDC/AAG-MAA/4084/2014), and CESAM (UIDP/50017/2020 + UIDB/50017/2020 + LA/P/0094/2020). S. Gomes is funded by a FCT research contract under the Scientific Employment Stimulus - Individual Call (CEEC Individual) - 2021.02867.CEECIND/CP1659/CT0004.

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© 2023

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