Aarhus Universitets segl

Three-layered control of mRNA poly(A) tail synthesis in Saccharomyces cerevisiae

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Dokumenter

DOI

  • Matti Turtola
  • ,
  • Cemre M. Manav
  • Ananthanarayanan Kumar, Medical Research Council
  • ,
  • Agnieszka Tudek, Institute of Biochemistry and Biophysics of the Polish Academy of Science
  • ,
  • Seweryn Mroczek, University of Warsaw, International Institute of Molecular and Cell Biology in Warsaw
  • ,
  • Pawel S. Krawczyk, International Institute of Molecular and Cell Biology in Warsaw
  • ,
  • Andrzej Dziembowski, University of Warsaw, International Institute of Molecular and Cell Biology in Warsaw
  • ,
  • Manfred Schmid
  • ,
  • Lori A. Passmore, Medical Research Council
  • ,
  • Ana Casanal, Medical Research Council
  • ,
  • Torben Heick Jensen

Biogenesis of most eukaryotic mRNAs involves the addition of an untemplated polyadenosine (pA) tail by the cleavage and polyadenylation machinery. The pA tail, and its exact length, impacts mRNA stability, nuclear export, and translation.To define how polyadenylation is controlled in S. cerevisiae, we have used an in vivo assay capable of assessing nuclear pA tail synthesis, analyzed tail length distributions by direct RNA sequencing, and reconstituted polyadenylation reactions with purified components. This revealed three control mechanisms for pA tail length. First, we found that the pA binding protein (PABP) Nab2p is the primary regulator of pA tail length. Second, when Nab2p is limiting, the nuclear pool of Pab1p, the second major PABP in yeast, controls the process. Third, when both PABPs are absent, the cleavage and polyadenylation factor (CPF) limits pA tail synthesis. Thus, Pab1p and CPF provide fail-safe mechanisms to a primary Nab2p-dependent pathway, thereby preventing uncontrolled polyadenylation and allowing mRNA export and translation.

OriginalsprogEngelsk
TidsskriftGenes and Development
Vol/bind35
Nummer17-18
Sider (fra-til)1290-1303
Antal sider14
ISSN0890-9369
DOI
StatusUdgivet - sep. 2021

Se relationer på Aarhus Universitet Citationsformater

ID: 223601365