The two enantiomers of citalopram bind to the human serotonin transporter in reversed orientations

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Standard

The two enantiomers of citalopram bind to the human serotonin transporter in reversed orientations. / Koldsø, Heidi; Severinsen, Kasper; Tran, Thuy Tien et al.

I: Journal of the American Chemical Society, Bind 132, Nr. 4, 2010, s. 1311-22.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Koldsø, H, Severinsen, K, Tran, TT, Celik, L, Jensen, HH, Wiborg, O, Schiøtt, B & Sinning, S 2010, 'The two enantiomers of citalopram bind to the human serotonin transporter in reversed orientations', Journal of the American Chemical Society, bind 132, nr. 4, s. 1311-22. https://doi.org/10.1021/ja906923j

APA

Koldsø, H., Severinsen, K., Tran, T. T., Celik, L., Jensen, H. H., Wiborg, O., Schiøtt, B., & Sinning, S. (2010). The two enantiomers of citalopram bind to the human serotonin transporter in reversed orientations. Journal of the American Chemical Society, 132(4), 1311-22. https://doi.org/10.1021/ja906923j

CBE

Koldsø H, Severinsen K, Tran TT, Celik L, Jensen HH, Wiborg O, Schiøtt B, Sinning S. 2010. The two enantiomers of citalopram bind to the human serotonin transporter in reversed orientations. Journal of the American Chemical Society. 132(4):1311-22. https://doi.org/10.1021/ja906923j

MLA

Koldsø, Heidi et al. "The two enantiomers of citalopram bind to the human serotonin transporter in reversed orientations". Journal of the American Chemical Society. 2010, 132(4). 1311-22. https://doi.org/10.1021/ja906923j

Vancouver

Koldsø H, Severinsen K, Tran TT, Celik L, Jensen HH, Wiborg O et al. The two enantiomers of citalopram bind to the human serotonin transporter in reversed orientations. Journal of the American Chemical Society. 2010;132(4):1311-22. https://doi.org/10.1021/ja906923j

Author

Koldsø, Heidi ; Severinsen, Kasper ; Tran, Thuy Tien et al. / The two enantiomers of citalopram bind to the human serotonin transporter in reversed orientations. I: Journal of the American Chemical Society. 2010 ; Bind 132, Nr. 4. s. 1311-22.

Bibtex

@article{47006d801ad911dfb95d000ea68e967b,
title = "The two enantiomers of citalopram bind to the human serotonin transporter in reversed orientations",
abstract = "The two enantiomers of the antidepressant citalopram inhibit the human serotonin transporter substantially differently. Previous studies revealed Tyr95 and Ile172 as important for citalopram binding, however, the overall orientation of the ligands in the binding site and the protein-ligand interaction points remain unknown. The binding of S- and R-citalopram to a human serotonin transporter homology model are herein examined via docking simulations including induced fit effects. For a better description of the formal charges of the ligand when bound inside the protein, polarization effects of the protein were included by additional quantum-polarized ligand docking calculations, where ligand charges are evaluated using QM/MM calculations. By this approach a much clearer picture emerged of the positions of the functional groups of citalopram. The two enantiomers are predicted to bind in the substrate binding pocket with opposite orientations of their aromatic groups. The predicted binding modes are experimentally validated using human wild type and 15 serotonin transporter mutants and 13 optically pure citalopram analogues. Important protein-ligand interaction points were identified validating one binding model for each enantiomer. In the validated model of the high affinity enantiomer, S-citalopram, the fluorine atom is located near Ala173 and Thr439 and the cyano group is in close proximity of Phe341; these contacts are found to be reversed for the R-enantiomer.",
author = "Heidi Kolds{\o} and Kasper Severinsen and Tran, {Thuy Tien} and Leyla Celik and Jensen, {Henrik Helligs{\o}} and Ove Wiborg and Birgit Schi{\o}tt and Steffen Sinning",
year = "2010",
doi = "10.1021/ja906923j",
language = "English",
volume = "132",
pages = "1311--22",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "ACS Publications",
number = "4",

}

RIS

TY - JOUR

T1 - The two enantiomers of citalopram bind to the human serotonin transporter in reversed orientations

AU - Koldsø, Heidi

AU - Severinsen, Kasper

AU - Tran, Thuy Tien

AU - Celik, Leyla

AU - Jensen, Henrik Helligsø

AU - Wiborg, Ove

AU - Schiøtt, Birgit

AU - Sinning, Steffen

PY - 2010

Y1 - 2010

N2 - The two enantiomers of the antidepressant citalopram inhibit the human serotonin transporter substantially differently. Previous studies revealed Tyr95 and Ile172 as important for citalopram binding, however, the overall orientation of the ligands in the binding site and the protein-ligand interaction points remain unknown. The binding of S- and R-citalopram to a human serotonin transporter homology model are herein examined via docking simulations including induced fit effects. For a better description of the formal charges of the ligand when bound inside the protein, polarization effects of the protein were included by additional quantum-polarized ligand docking calculations, where ligand charges are evaluated using QM/MM calculations. By this approach a much clearer picture emerged of the positions of the functional groups of citalopram. The two enantiomers are predicted to bind in the substrate binding pocket with opposite orientations of their aromatic groups. The predicted binding modes are experimentally validated using human wild type and 15 serotonin transporter mutants and 13 optically pure citalopram analogues. Important protein-ligand interaction points were identified validating one binding model for each enantiomer. In the validated model of the high affinity enantiomer, S-citalopram, the fluorine atom is located near Ala173 and Thr439 and the cyano group is in close proximity of Phe341; these contacts are found to be reversed for the R-enantiomer.

AB - The two enantiomers of the antidepressant citalopram inhibit the human serotonin transporter substantially differently. Previous studies revealed Tyr95 and Ile172 as important for citalopram binding, however, the overall orientation of the ligands in the binding site and the protein-ligand interaction points remain unknown. The binding of S- and R-citalopram to a human serotonin transporter homology model are herein examined via docking simulations including induced fit effects. For a better description of the formal charges of the ligand when bound inside the protein, polarization effects of the protein were included by additional quantum-polarized ligand docking calculations, where ligand charges are evaluated using QM/MM calculations. By this approach a much clearer picture emerged of the positions of the functional groups of citalopram. The two enantiomers are predicted to bind in the substrate binding pocket with opposite orientations of their aromatic groups. The predicted binding modes are experimentally validated using human wild type and 15 serotonin transporter mutants and 13 optically pure citalopram analogues. Important protein-ligand interaction points were identified validating one binding model for each enantiomer. In the validated model of the high affinity enantiomer, S-citalopram, the fluorine atom is located near Ala173 and Thr439 and the cyano group is in close proximity of Phe341; these contacts are found to be reversed for the R-enantiomer.

U2 - 10.1021/ja906923j

DO - 10.1021/ja906923j

M3 - Journal article

C2 - 20055463

VL - 132

SP - 1311

EP - 1322

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 4

ER -