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The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans

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Standard

The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans. / Mørch, Maria G.M.; Møller, Katrine V.; Hesselager, Marianne O.; Harders, Rikke H.; Kidmose, Caroline L.; Buhl, Therese; Fuursted, Kurt; Bendixen, Emøke; Shen, Chong; Christensen, Lotte G.; Poulsen, Charlotte H.; Olsen, Anders.

I: Scientific Reports, Bind 11, 10717, 12.2021.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Mørch, MGM, Møller, KV, Hesselager, MO, Harders, RH, Kidmose, CL, Buhl, T, Fuursted, K, Bendixen, E, Shen, C, Christensen, LG, Poulsen, CH & Olsen, A 2021, 'The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans', Scientific Reports, bind 11, 10717. https://doi.org/10.1038/s41598-021-89831-y

APA

Mørch, M. G. M., Møller, K. V., Hesselager, M. O., Harders, R. H., Kidmose, C. L., Buhl, T., Fuursted, K., Bendixen, E., Shen, C., Christensen, L. G., Poulsen, C. H., & Olsen, A. (2021). The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans. Scientific Reports, 11, [10717]. https://doi.org/10.1038/s41598-021-89831-y

CBE

Mørch MGM, Møller KV, Hesselager MO, Harders RH, Kidmose CL, Buhl T, Fuursted K, Bendixen E, Shen C, Christensen LG, Poulsen CH, Olsen A. 2021. The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans. Scientific Reports. 11:Article 10717. https://doi.org/10.1038/s41598-021-89831-y

MLA

Vancouver

Mørch MGM, Møller KV, Hesselager MO, Harders RH, Kidmose CL, Buhl T o.a. The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans. Scientific Reports. 2021 dec;11. 10717. https://doi.org/10.1038/s41598-021-89831-y

Author

Mørch, Maria G.M. ; Møller, Katrine V. ; Hesselager, Marianne O. ; Harders, Rikke H. ; Kidmose, Caroline L. ; Buhl, Therese ; Fuursted, Kurt ; Bendixen, Emøke ; Shen, Chong ; Christensen, Lotte G. ; Poulsen, Charlotte H. ; Olsen, Anders. / The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans. I: Scientific Reports. 2021 ; Bind 11.

Bibtex

@article{9d216f1e952741a59c7b4b79166b9d5d,
title = "The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans",
abstract = "Worldwide the increase in multi-resistant bacteria due to misuse of traditional antibiotics is a growing threat for our health. Finding alternatives to traditional antibiotics is thus timely. Probiotic bacteria have numerous beneficial effects and could offer safer alternatives to traditional antibiotics. Here, we use the nematode Caenorhabditis elegans (C. elegans) to screen a library of different lactobacilli to identify potential probiotic bacteria and characterize their mechanisms of action. We show that pretreatment with the Lactobacillus spp. Lb21 increases lifespan of C. elegans and results in resistance towards pathogenic methicillin-resistant Staphylococcus aureus (MRSA). Using genetic analysis, we find that Lb21-mediated MRSA resistance is dependent on the DBL-1 ligand of the TGF-β signaling pathway in C. elegans. This response is evolutionarily conserved as we find that Lb21 also induces the TGF-β pathway in porcine epithelial cells. We further characterize the host responses in an unbiased proteome analysis and identify 474 proteins regulated in worms fed Lb21 compared to control food. These include fatty acid CoA synthetase ACS-22, aspartic protease ASP-6 and vitellogenin VIT-2 which are important for Lb21-mediated MRSA resistance. Thus, Lb21 exerts its probiotic effect on C. elegans in a multifactorial manner. In summary, our study establishes a mechanistic basis for the antimicrobial potential of lactobacilli.",
author = "M{\o}rch, {Maria G.M.} and M{\o}ller, {Katrine V.} and Hesselager, {Marianne O.} and Harders, {Rikke H.} and Kidmose, {Caroline L.} and Therese Buhl and Kurt Fuursted and Em{\o}ke Bendixen and Chong Shen and Christensen, {Lotte G.} and Poulsen, {Charlotte H.} and Anders Olsen",
note = "Publisher Copyright: {\textcopyright} 2021, The Author(s). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
month = dec,
doi = "10.1038/s41598-021-89831-y",
language = "English",
volume = "11",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - The TGF-β ligand DBL-1 is a key player in a multifaceted probiotic protection against MRSA in C. elegans

AU - Mørch, Maria G.M.

AU - Møller, Katrine V.

AU - Hesselager, Marianne O.

AU - Harders, Rikke H.

AU - Kidmose, Caroline L.

AU - Buhl, Therese

AU - Fuursted, Kurt

AU - Bendixen, Emøke

AU - Shen, Chong

AU - Christensen, Lotte G.

AU - Poulsen, Charlotte H.

AU - Olsen, Anders

N1 - Publisher Copyright: © 2021, The Author(s). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/12

Y1 - 2021/12

N2 - Worldwide the increase in multi-resistant bacteria due to misuse of traditional antibiotics is a growing threat for our health. Finding alternatives to traditional antibiotics is thus timely. Probiotic bacteria have numerous beneficial effects and could offer safer alternatives to traditional antibiotics. Here, we use the nematode Caenorhabditis elegans (C. elegans) to screen a library of different lactobacilli to identify potential probiotic bacteria and characterize their mechanisms of action. We show that pretreatment with the Lactobacillus spp. Lb21 increases lifespan of C. elegans and results in resistance towards pathogenic methicillin-resistant Staphylococcus aureus (MRSA). Using genetic analysis, we find that Lb21-mediated MRSA resistance is dependent on the DBL-1 ligand of the TGF-β signaling pathway in C. elegans. This response is evolutionarily conserved as we find that Lb21 also induces the TGF-β pathway in porcine epithelial cells. We further characterize the host responses in an unbiased proteome analysis and identify 474 proteins regulated in worms fed Lb21 compared to control food. These include fatty acid CoA synthetase ACS-22, aspartic protease ASP-6 and vitellogenin VIT-2 which are important for Lb21-mediated MRSA resistance. Thus, Lb21 exerts its probiotic effect on C. elegans in a multifactorial manner. In summary, our study establishes a mechanistic basis for the antimicrobial potential of lactobacilli.

AB - Worldwide the increase in multi-resistant bacteria due to misuse of traditional antibiotics is a growing threat for our health. Finding alternatives to traditional antibiotics is thus timely. Probiotic bacteria have numerous beneficial effects and could offer safer alternatives to traditional antibiotics. Here, we use the nematode Caenorhabditis elegans (C. elegans) to screen a library of different lactobacilli to identify potential probiotic bacteria and characterize their mechanisms of action. We show that pretreatment with the Lactobacillus spp. Lb21 increases lifespan of C. elegans and results in resistance towards pathogenic methicillin-resistant Staphylococcus aureus (MRSA). Using genetic analysis, we find that Lb21-mediated MRSA resistance is dependent on the DBL-1 ligand of the TGF-β signaling pathway in C. elegans. This response is evolutionarily conserved as we find that Lb21 also induces the TGF-β pathway in porcine epithelial cells. We further characterize the host responses in an unbiased proteome analysis and identify 474 proteins regulated in worms fed Lb21 compared to control food. These include fatty acid CoA synthetase ACS-22, aspartic protease ASP-6 and vitellogenin VIT-2 which are important for Lb21-mediated MRSA resistance. Thus, Lb21 exerts its probiotic effect on C. elegans in a multifactorial manner. In summary, our study establishes a mechanistic basis for the antimicrobial potential of lactobacilli.

UR - http://www.scopus.com/inward/record.url?scp=85106656665&partnerID=8YFLogxK

U2 - 10.1038/s41598-021-89831-y

DO - 10.1038/s41598-021-89831-y

M3 - Journal article

C2 - 34021197

AN - SCOPUS:85106656665

VL - 11

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 10717

ER -