The strength of the HIV-1 3' splice sites affects Rev function

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • Susanne Kammler, Danmark
  • Marianne Otte, Institut für Virologie, Heinrich-Heine-Universität Düsseldorf, Tyskland
  • Ilona Hauber, Heinrich-Pette-Institute for Experimental Virology and Immunology, Tyskland
  • Jørgen Kjems
  • Joachim Hauber, Institut für Virologie, Heinrich-Heine-Universität Düsseldorf, Tyskland
  • Heiner Schaal, Institut für Virologie, Heinrich-Heine-Universität Düsseldorf, Tyskland
The HIV-1 Rev protein is a key component in the early to late switch in HIV-1 splicing from early intronless (e.g. tat, rev) to late intron-containing Rev-dependent (e.g. gag, vif, env) transcripts. Previous results suggested that cis-acting sequences and inefficient 5' and 3' splice sites are a prerequisite for Rev function. However, we and other groups have shown that two of the HIV-1 5' splice sites, D1 and D4, are efficiently used in vitro and in vivo. Here, we focus on the efficiency of the HIV-1 3' splice sites taking into consideration to what extent their intrinsic efficiencies are modulated by their downstream cis-acting exonic sequences. Furthermore, we delineate their role in RNA stabilization and Rev function.
OriginalsprogEngelsk
TidsskriftRetrovirology
Vol/bind3
Nummer89
Sider (fra-til)1-20
Antal sider20
ISSN1742-4690
DOI
StatusUdgivet - dec. 2006

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