The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought

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The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought. / Holmstrup, Martin.

I: Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology, Bind 188, Nr. 6, 11.2018, s. 939-945.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Holmstrup, M 2018, 'The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought', Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology, bind 188, nr. 6, s. 939-945. https://doi.org/10.1007/s00360-018-1180-3

APA

Holmstrup, M. (2018). The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought. Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology, 188(6), 939-945. https://doi.org/10.1007/s00360-018-1180-3

CBE

Holmstrup M. 2018. The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought. Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology. 188(6):939-945. https://doi.org/10.1007/s00360-018-1180-3

MLA

Holmstrup, Martin. "The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought". Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology. 2018, 188(6). 939-945. https://doi.org/10.1007/s00360-018-1180-3

Vancouver

Holmstrup M. The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought. Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology. 2018 nov;188(6):939-945. https://doi.org/10.1007/s00360-018-1180-3

Author

Holmstrup, Martin. / The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought. I: Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology. 2018 ; Bind 188, Nr. 6. s. 939-945.

Bibtex

@article{858aef7910df465097351f22291b0904,
title = "The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought",
abstract = "The springtail Megaphorura arctica Tullberg 1876 is widespread in the arctic and subarctic regions where it can be abundant along beaches. This species survives winters using cryoprotective dehydration as a cold tolerance strategy during which it becomes drastically dehydrated. Several studies have investigated the physiological responses associated with water loss of M. arctica under exposure to freezing temperatures, but little is known of the dynamics of body water and hemolymph osmolality when subjected to gradually increasing drought stress at temperatures above the freezing point. Therefore, an experiment was conducted in which M. arctica was subjected to relative humidities (RH) decreasing from fully saturated conditions to ca. 89%RH over a period of 30 days. During the experiment water content of springtails decreased from about 3 to ca. 0.5mgmg(-1) dry weight. Alongside with water loss, trehalose concentrations increased from nearly nothing to 0.12mg mg(-1) dry weight, which contributed to an increase in hemolymph osmolality from ca. 250mOsm to at least 7Osm. All springtails survived water loss down to 0.7mgmg(-1) dry weight and hemolymph osmolality of ca. 4Osm, and about 60% of the springtails survived with only 0.5mg watermg(-1) dry weight and osmolality of ca. 7Osm.At this level of dehydration, Differential Scanning Calorimetry analysis showed that most, but not all, osmotically active water was lost. It is discussed that the extensive dehydration must be associated with high concentrations of salts potentially causing denaturation and precipitation of cellular proteins. M. arctica is remarkably tolerant of dehydration, but because it does not endure loss of the osmotically inactive water it cannot be categorized as a truly anhydrobiotic species.",
keywords = "Anhydrobiosis, Collembola, Desiccation, Differential scanning calorimetry, Osmotic response, TULLBERG 1876 ONYCHIURIDAE, WATER-VAPOR ABSORPTION, CRYOPROTECTIVE DEHYDRATION, FOLSOMIA-CANDIDA, COLLEMBOLA, TOLERANCE, DESICCATION, COLD, ANHYDROBIOSIS, ACCUMULATION",
author = "Martin Holmstrup",
year = "2018",
month = nov,
doi = "10.1007/s00360-018-1180-3",
language = "English",
volume = "188",
pages = "939--945",
journal = "Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology",
issn = "0174-1578",
publisher = "Springer",
number = "6",

}

RIS

TY - JOUR

T1 - The springtail Megaphorura arctica survives extremely high osmolality of body fluids during drought

AU - Holmstrup, Martin

PY - 2018/11

Y1 - 2018/11

N2 - The springtail Megaphorura arctica Tullberg 1876 is widespread in the arctic and subarctic regions where it can be abundant along beaches. This species survives winters using cryoprotective dehydration as a cold tolerance strategy during which it becomes drastically dehydrated. Several studies have investigated the physiological responses associated with water loss of M. arctica under exposure to freezing temperatures, but little is known of the dynamics of body water and hemolymph osmolality when subjected to gradually increasing drought stress at temperatures above the freezing point. Therefore, an experiment was conducted in which M. arctica was subjected to relative humidities (RH) decreasing from fully saturated conditions to ca. 89%RH over a period of 30 days. During the experiment water content of springtails decreased from about 3 to ca. 0.5mgmg(-1) dry weight. Alongside with water loss, trehalose concentrations increased from nearly nothing to 0.12mg mg(-1) dry weight, which contributed to an increase in hemolymph osmolality from ca. 250mOsm to at least 7Osm. All springtails survived water loss down to 0.7mgmg(-1) dry weight and hemolymph osmolality of ca. 4Osm, and about 60% of the springtails survived with only 0.5mg watermg(-1) dry weight and osmolality of ca. 7Osm.At this level of dehydration, Differential Scanning Calorimetry analysis showed that most, but not all, osmotically active water was lost. It is discussed that the extensive dehydration must be associated with high concentrations of salts potentially causing denaturation and precipitation of cellular proteins. M. arctica is remarkably tolerant of dehydration, but because it does not endure loss of the osmotically inactive water it cannot be categorized as a truly anhydrobiotic species.

AB - The springtail Megaphorura arctica Tullberg 1876 is widespread in the arctic and subarctic regions where it can be abundant along beaches. This species survives winters using cryoprotective dehydration as a cold tolerance strategy during which it becomes drastically dehydrated. Several studies have investigated the physiological responses associated with water loss of M. arctica under exposure to freezing temperatures, but little is known of the dynamics of body water and hemolymph osmolality when subjected to gradually increasing drought stress at temperatures above the freezing point. Therefore, an experiment was conducted in which M. arctica was subjected to relative humidities (RH) decreasing from fully saturated conditions to ca. 89%RH over a period of 30 days. During the experiment water content of springtails decreased from about 3 to ca. 0.5mgmg(-1) dry weight. Alongside with water loss, trehalose concentrations increased from nearly nothing to 0.12mg mg(-1) dry weight, which contributed to an increase in hemolymph osmolality from ca. 250mOsm to at least 7Osm. All springtails survived water loss down to 0.7mgmg(-1) dry weight and hemolymph osmolality of ca. 4Osm, and about 60% of the springtails survived with only 0.5mg watermg(-1) dry weight and osmolality of ca. 7Osm.At this level of dehydration, Differential Scanning Calorimetry analysis showed that most, but not all, osmotically active water was lost. It is discussed that the extensive dehydration must be associated with high concentrations of salts potentially causing denaturation and precipitation of cellular proteins. M. arctica is remarkably tolerant of dehydration, but because it does not endure loss of the osmotically inactive water it cannot be categorized as a truly anhydrobiotic species.

KW - Anhydrobiosis

KW - Collembola

KW - Desiccation

KW - Differential scanning calorimetry

KW - Osmotic response

KW - TULLBERG 1876 ONYCHIURIDAE

KW - WATER-VAPOR ABSORPTION

KW - CRYOPROTECTIVE DEHYDRATION

KW - FOLSOMIA-CANDIDA

KW - COLLEMBOLA

KW - TOLERANCE

KW - DESICCATION

KW - COLD

KW - ANHYDROBIOSIS

KW - ACCUMULATION

U2 - 10.1007/s00360-018-1180-3

DO - 10.1007/s00360-018-1180-3

M3 - Journal article

C2 - 30194462

VL - 188

SP - 939

EP - 945

JO - Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology

JF - Journal of Comparative Physiology B: Biochemical, Systems, and Environmental Physiology

SN - 0174-1578

IS - 6

ER -