The Sheaths of Methanospirillum Are Made of a New Type of Amyloid Protein

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The Sheaths of Methanospirillum Are Made of a New Type of Amyloid Protein. / Christensen, Line Friis Bakmann; Hansen, Lonnie Maria; Finster, Kai; Christiansen, Gunna; Nielsen, Per Halkjaer; Otzen, Daniel Erik; Dueholm, Morten Simonsen.

I: Frontiers in Microbiology, Bind 9, 2729, 2018.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Christensen, Line Friis Bakmann ; Hansen, Lonnie Maria ; Finster, Kai ; Christiansen, Gunna ; Nielsen, Per Halkjaer ; Otzen, Daniel Erik ; Dueholm, Morten Simonsen. / The Sheaths of Methanospirillum Are Made of a New Type of Amyloid Protein. I: Frontiers in Microbiology. 2018 ; Bind 9.

Bibtex

@article{8d30ebbc9f274fa1ac9db6bf2c20f45f,
title = "The Sheaths of Methanospirillum Are Made of a New Type of Amyloid Protein",
abstract = "The genera Methanospirillum and Methanosaeta contain species of anaerobic archaea that grow and divide within proteinaceous tubular sheaths that protect them from environmental stressors. The sheaths of Methanosaeta thermophila PT are composed of the 60.9 kDa major sheath protein MspA. In this study we show that sheaths purified from Methanospirillum hungatei JF-1 are regularly striated tubular structures with amyloid-like properties similar to those of M. thermophila PT. Depolymerizing the sheaths from M. hungatei JF-1 allowed us to identify a 40.6 kDa protein (WP_011449234.1) that shares 23{\%} sequence similarity to MspA from M. thermophila PT (ABK14853.1), indicating that they might be distant homologs. The genome of M. hungatei JF-1 encodes six homologs of the identified MspA protein. Several homologs also exist in the related strains Methanospirillum stamsii Pt1 (7 homologs, 28-66{\%} sequence identity), M. lacunae Ki8-1 C (15 homologs, 29-60{\%} sequence identity) and Methanolinea tarda NOBI-1 (2 homologs, 31{\%} sequence identity). The MspA protein discovered here could accordingly represent a more widely found sheath protein than the MspA from M. thermophila PT, which currently has no homologs in the NCBI Reference Sequence database (RefSeq).",
keywords = "archaea, methanogen, functional amyloid, sheath protein, homologs, HUNGATEI GP1, METHANOGENIC BACTERIA, METHANOTHRIX-CONCILII, EPITOPE, CONFORMATION, PSEUDOMONAS, BIOGENESIS, ABUNDANCE, PEPTIDES, ADHESINS",
author = "Christensen, {Line Friis Bakmann} and Hansen, {Lonnie Maria} and Kai Finster and Gunna Christiansen and Nielsen, {Per Halkjaer} and Otzen, {Daniel Erik} and Dueholm, {Morten Simonsen}",
year = "2018",
doi = "10.3389/fmicb.2018.02729",
language = "English",
volume = "9",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A",

}

RIS

TY - JOUR

T1 - The Sheaths of Methanospirillum Are Made of a New Type of Amyloid Protein

AU - Christensen, Line Friis Bakmann

AU - Hansen, Lonnie Maria

AU - Finster, Kai

AU - Christiansen, Gunna

AU - Nielsen, Per Halkjaer

AU - Otzen, Daniel Erik

AU - Dueholm, Morten Simonsen

PY - 2018

Y1 - 2018

N2 - The genera Methanospirillum and Methanosaeta contain species of anaerobic archaea that grow and divide within proteinaceous tubular sheaths that protect them from environmental stressors. The sheaths of Methanosaeta thermophila PT are composed of the 60.9 kDa major sheath protein MspA. In this study we show that sheaths purified from Methanospirillum hungatei JF-1 are regularly striated tubular structures with amyloid-like properties similar to those of M. thermophila PT. Depolymerizing the sheaths from M. hungatei JF-1 allowed us to identify a 40.6 kDa protein (WP_011449234.1) that shares 23% sequence similarity to MspA from M. thermophila PT (ABK14853.1), indicating that they might be distant homologs. The genome of M. hungatei JF-1 encodes six homologs of the identified MspA protein. Several homologs also exist in the related strains Methanospirillum stamsii Pt1 (7 homologs, 28-66% sequence identity), M. lacunae Ki8-1 C (15 homologs, 29-60% sequence identity) and Methanolinea tarda NOBI-1 (2 homologs, 31% sequence identity). The MspA protein discovered here could accordingly represent a more widely found sheath protein than the MspA from M. thermophila PT, which currently has no homologs in the NCBI Reference Sequence database (RefSeq).

AB - The genera Methanospirillum and Methanosaeta contain species of anaerobic archaea that grow and divide within proteinaceous tubular sheaths that protect them from environmental stressors. The sheaths of Methanosaeta thermophila PT are composed of the 60.9 kDa major sheath protein MspA. In this study we show that sheaths purified from Methanospirillum hungatei JF-1 are regularly striated tubular structures with amyloid-like properties similar to those of M. thermophila PT. Depolymerizing the sheaths from M. hungatei JF-1 allowed us to identify a 40.6 kDa protein (WP_011449234.1) that shares 23% sequence similarity to MspA from M. thermophila PT (ABK14853.1), indicating that they might be distant homologs. The genome of M. hungatei JF-1 encodes six homologs of the identified MspA protein. Several homologs also exist in the related strains Methanospirillum stamsii Pt1 (7 homologs, 28-66% sequence identity), M. lacunae Ki8-1 C (15 homologs, 29-60% sequence identity) and Methanolinea tarda NOBI-1 (2 homologs, 31% sequence identity). The MspA protein discovered here could accordingly represent a more widely found sheath protein than the MspA from M. thermophila PT, which currently has no homologs in the NCBI Reference Sequence database (RefSeq).

KW - archaea

KW - methanogen

KW - functional amyloid

KW - sheath protein

KW - homologs

KW - HUNGATEI GP1

KW - METHANOGENIC BACTERIA

KW - METHANOTHRIX-CONCILII

KW - EPITOPE

KW - CONFORMATION

KW - PSEUDOMONAS

KW - BIOGENESIS

KW - ABUNDANCE

KW - PEPTIDES

KW - ADHESINS

UR - http://www.scopus.com/inward/record.url?scp=85056734961&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2018.02729

DO - 10.3389/fmicb.2018.02729

M3 - Journal article

VL - 9

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 2729

ER -