The Role of Petrimonas mucosa ING2-E5AT in Mesophilic Biogas Reactor Systems as Deduced from Multiomics Analyses

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  • Irena Maus, Bielefeld University
  • ,
  • Tom Tubbesing, Bielefeld University
  • ,
  • Daniel Wibberg, Bielefeld University
  • ,
  • Robert Heyer, Otto von Guericke Unversity Magdeburg
  • ,
  • Julia Hassa, Bielefeld University, Leibniz Institute for Agriculture and Engineering, Potsdam
  • ,
  • Geizecler Tomazetto
  • Liren Huang, Bielefeld University
  • ,
  • Boyke Bunk, Leibniz Institute DSMZ-German Collection of Microorganisms, Cell Cultures GmbH
  • ,
  • Cathrin Spröer, Leibniz Institute DSMZ-German Collection of Microorganisms, Cell Cultures GmbH
  • ,
  • Dirk Benndorf, Otto von Guericke University, Max Planck Institute for Dynamics of Complex Technical Systems, Anhalt University of Applied Sciences
  • ,
  • Vladimir Zverlov, Technical University of Munich (TUM), National Research Center “Kurchatov Institute,”
  • ,
  • Alfred Pühler, Bielefeld University
  • ,
  • Michael Klocke, Institute of Agricultural and Urban Ecological Projects Affiliated to Berlin Humboldt University (IASP
  • ,
  • Alexander Sczyrba, Bielefeld University
  • ,
  • Andreas Schlüter, Bielefeld University

Members of the genera Proteiniphilum and Petrimonas were speculated to represent indicators reflecting process instability within anaerobic digestion (AD) microbiomes. Therefore, Petrimonas mucosa ING2-E5A T was isolated from a biogas reactor sample and sequenced on the PacBio RSII and Illumina MiSeq sequencers. Phylogenetic classification positioned the strain ING2-E5A T in close proximity to Fermentimonas and Proteiniphilum species (family Dysgonomonadaceae). ING2-E5A T encodes a number of genes for glycosyl-hydrolyses (GH) which are organized in Polysaccharide Utilization Loci (PUL) comprising tandem susCD-like genes for a TonB-dependent outer-membrane transporter and a cell surface glycan-binding protein. Different GHs encoded in PUL are involved in pectin degradation, reflecting a pronounced specialization of the ING2-E5A T PUL systems regarding the decomposition of this polysaccharide. Genes encoding enzymes participating in amino acids fermentation were also identified. Fragment recruitments with the ING2-E5A T genome as a template and publicly available metagenomes of AD microbiomes revealed that Petrimonas species are present in 146 out of 257 datasets supporting their importance in AD microbiomes. Metatranscriptome analyses of AD microbiomes uncovered active sugar and amino acid fermentation pathways for Petrimonas species. Likewise, screening of metaproteome datasets demonstrated expression of the Petrimonas PUL-specific component SusC providing further evidence that PUL play a central role for the lifestyle of Petrimonas species.

OriginalsprogEngelsk
Artikelnummer2024
TidsskriftMicroorganisms
Vol/bind8
Nummer12
Antal sider23
ISSN2076-2607
DOI
StatusUdgivet - dec. 2020

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