TY - JOUR
T1 - The protein composition of the digestive fluid from the Venus flytrap sheds light on prey digestion mechanisms
AU - Schulze, Waltraud X
AU - Sanggaard, Kristian Wejse
AU - Kreuzer, Ines
AU - Knudsen, Anders D
AU - Bemm, Felix
AU - Thøgersen, Ida B
AU - Brautigam, Andrea
AU - Thomsen, Line R
AU - Schliesky, Simon
AU - Dyrlund, Thomas Franck
AU - Escalante-Perez, Maria
AU - Becker, Dirk
AU - Schultz, Jörg
AU - Karring, Henrik
AU - Weber, Andreas
AU - Højrup, Peter
AU - Hedrich, Rainer
AU - Enghild, Jan Johannes
PY - 2012/11/1
Y1 - 2012/11/1
N2 - The Venus flytrap (Dionaea muscipula) is one of the most well-known carnivorous plants because of its unique ability to capture small animals, usually insects or spiders, through a unique snap-trapping mechanism. The animals are subsequently killed and digested to assimilate nutrients as the plants grow in mineral-deficient soils. We deep sequenced the cDNA from Dionaea traps to obtain transcript libraries, which were used in the mass spectrometry-based identification of the proteins secreted during digestion. The identified proteins consisted of peroxidases, nucleases, phosphatases, phospholipases, a glucanase, chitinases, and proteolytic enzymes, including four cysteine proteases, two aspartic proteases, and a serine carboxypeptidase. The majority of the most abundant proteins were categorized as pathogenesis-related proteins, suggesting that the plant's digestive system evolved from defense-related processes. This in-depth characterization of a highly specialized secreted fluid from a carnivorous plant provides new information about its prey digestion mechanism and the evolutionary processes driving its defense pathways and nutrient acquisition.
AB - The Venus flytrap (Dionaea muscipula) is one of the most well-known carnivorous plants because of its unique ability to capture small animals, usually insects or spiders, through a unique snap-trapping mechanism. The animals are subsequently killed and digested to assimilate nutrients as the plants grow in mineral-deficient soils. We deep sequenced the cDNA from Dionaea traps to obtain transcript libraries, which were used in the mass spectrometry-based identification of the proteins secreted during digestion. The identified proteins consisted of peroxidases, nucleases, phosphatases, phospholipases, a glucanase, chitinases, and proteolytic enzymes, including four cysteine proteases, two aspartic proteases, and a serine carboxypeptidase. The majority of the most abundant proteins were categorized as pathogenesis-related proteins, suggesting that the plant's digestive system evolved from defense-related processes. This in-depth characterization of a highly specialized secreted fluid from a carnivorous plant provides new information about its prey digestion mechanism and the evolutionary processes driving its defense pathways and nutrient acquisition.
U2 - 10.1074/mcp.M112.021006
DO - 10.1074/mcp.M112.021006
M3 - Journal article
C2 - 22891002
SN - 1535-9476
VL - 11
SP - 1306
EP - 1319
JO - Molecular and Cellular Proteomics
JF - Molecular and Cellular Proteomics
IS - 11
ER -