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The phosphorylated sodium chloride cotransporter in urinary exosomes is superior to prostasin as a marker for aldosteronism

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  • Nils van der Lubbe
  • ,
  • Pieter M Jansen
  • ,
  • Mahdi Salih
  • ,
  • Robert A. Fenton
  • Anton H van den Meiracker
  • ,
  • A H Jan Danser
  • ,
  • Robert Zietse
  • ,
  • Ewout J Hoorn
Urinary exosomes are vesicles derived from renal tubular epithelial cells. Exosomes often contain several disease-associated proteins and are thus useful targets for identifying biomarkers of disease. Here, we hypothesized that the phosphorylated (active) form of the sodium chloride cotransporter (pNCC) or prostasin could serve as biomarkers for aldosteronism. We tested this in 2 animal models of aldosteronism (aldosterone infusion or low-sodium diet) and in patients with primary aldosteronism. Urinary exosomes were isolated from 24-hour urine or spot urine using ultracentrifugation. In rats, a normal or a high dose of aldosterone for 2, 3, or 8 days increased pNCC 3-fold in urinary exosomes (P
OriginalsprogEngelsk
TidsskriftHypertension
Vol/bind60
Nummer3
Sider (fra-til)741-8
Antal sider8
ISSN0194-911X
DOI
StatusUdgivet - 2012

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