The number of glomeruli and pyruvate metabolism is not strongly coupled in the healthy rat kidney

TY - GEN

T1 - The number of glomeruli and pyruvate metabolism is not strongly coupled in the healthy rat kidney

AU - Bech, Sabrina Kahina

AU - Qi, Haiyun

AU - Mariager, Christian Østergaard

AU - Hansen, Esben Søvsø Szocska

AU - Ilicak, Efe

AU - Zöllner, Frank G

AU - Laustsen, Christoffer

N1 - © 2021 International Society for Magnetic Resonance in Medicine.

PY - 2022/2

Y1 - 2022/2

N2 - PURPOSE: The number of glomeruli is different in men and women, as they also present different prevalence and progression of chronic kidney disease. A recent study has demonstrated a potential difference in renal metabolism between sexes, and a potential explanation could be the differences in glomeruli number. This study investigates the potential correlation between glomerular number and pyruvate metabolism in healthy kidneys.METHODS: This study is an experimental study with rats (N = 12). We used cationized-ferritin MRI to visualize and count glomeruli and hyperpolarized [1-13 C]pyruvate to map the metabolism. Dynamic contrast-enhanced MRI was used to analyze kidney hemodynamics using gadolinium tracer.RESULTS: Data showed no or subtle correlation between the number of glomeruli and the pyruvate metabolism. Minor differences were observed in the number of glomeruli (female = 24,509 vs. male = 26 350; p = .16), renal plasma flow (female = 606.6 vs. male= 455.7 ml/min/100 g; p = .18), and volume of distribution (female = 87.44 vs. male = 76.61 ml/100 ml; p = .54) between sexes. Mean transit time was significantly prolonged in males compared with females (female = 8.868 s vs. male = 10.63 s; p = .04).CONCLUSION: No strong statistically correlation between the number of glomeruli and the pyruvate metabolism was found in healthy rat kidneys.

AB - PURPOSE: The number of glomeruli is different in men and women, as they also present different prevalence and progression of chronic kidney disease. A recent study has demonstrated a potential difference in renal metabolism between sexes, and a potential explanation could be the differences in glomeruli number. This study investigates the potential correlation between glomerular number and pyruvate metabolism in healthy kidneys.METHODS: This study is an experimental study with rats (N = 12). We used cationized-ferritin MRI to visualize and count glomeruli and hyperpolarized [1-13 C]pyruvate to map the metabolism. Dynamic contrast-enhanced MRI was used to analyze kidney hemodynamics using gadolinium tracer.RESULTS: Data showed no or subtle correlation between the number of glomeruli and the pyruvate metabolism. Minor differences were observed in the number of glomeruli (female = 24,509 vs. male = 26 350; p = .16), renal plasma flow (female = 606.6 vs. male= 455.7 ml/min/100 g; p = .18), and volume of distribution (female = 87.44 vs. male = 76.61 ml/100 ml; p = .54) between sexes. Mean transit time was significantly prolonged in males compared with females (female = 8.868 s vs. male = 10.63 s; p = .04).CONCLUSION: No strong statistically correlation between the number of glomeruli and the pyruvate metabolism was found in healthy rat kidneys.

KW - MRI

KW - hyperpolarization

KW - renal

KW - sex

UR - http://www.scopus.com/inward/record.url?scp=85115416628&partnerID=8YFLogxK

U2 - 10.1002/mrm.29025

DO - 10.1002/mrm.29025

M3 - Conference article

C2 - 34554602

SN - 0740-3194

VL - 87

SP - 896

EP - 903

JO - Magnetic Resonance in Medicine

JF - Magnetic Resonance in Medicine

IS - 2

ER -