The NSD3L histone methyltransferase regulates cell cycle and cell invasion in breast cancer cells

Zhangle Zhou; Rune Thomsen; Søren Kahns; Anders Lade Nielsen; Anders Lade Nielsen

doi:10.1016/j.bbrc.2010.06.119

The NSD3L histone methyltransferase regulates cell cycle and cell invasion in breast cancer cells

Zhangle Zhou, Rune Thomsen, Søren Kahns, Anders Lade Nielsen, Anders Lade Nielsen

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

33 Citationer (Scopus)

Abstract

NSD3/WHSC1L1 histone methyltransferase gene aberrations are observed in leukemia and in breast and lung carcinomas, suggesting that NSD3 is implicated in carcinogenesis. In this study we examined in human breast cancer cells the NSD3L isoform which contains the catalytic histone methyltransferase SET-domain. siRNA directed depletion of NSD3L followed by genome-wide microarray analysis identified NSD3L regulated genes which could be functionally linked to cellular signaling pathways such as cell growth, cell cycle, cell motility, transcription, and apoptosis. Notably up-regulated genes are the cell cycle regulators E2F2 and Arl2. In accordance with a function of NSD3L in cell cycle regulation NSD3L depletion resulted in an increase in the number of cells in the S and G2/M cell cycle phases. Moreover, NSD3L depletion increased the invasiveness of MDA-MB-231 breast cancer cells indicating that NSD3L normally restrain cellular metastatic potential. Together the presented data indicates that NSD3L is a candidate tumor suppressor.

Originalsprog	Engelsk
Tidsskrift	Biochemical and Biophysical Research Communications
Vol/bind	398
Nummer	3
Sider (fra-til)	565-570
ISSN	0006-291X
DOI	https://doi.org/10.1016/j.bbrc.2010.06.119
Status	Udgivet - 30 jul. 2010

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10.1016/j.bbrc.2010.06.119

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title = "The NSD3L histone methyltransferase regulates cell cycle and cell invasion in breast cancer cells",

abstract = "NSD3/WHSC1L1 histone methyltransferase gene aberrations are observed in leukemia and in breast and lung carcinomas, suggesting that NSD3 is implicated in carcinogenesis. In this study we examined in human breast cancer cells the NSD3L isoform which contains the catalytic histone methyltransferase SET-domain. siRNA directed depletion of NSD3L followed by genome-wide microarray analysis identified NSD3L regulated genes which could be functionally linked to cellular signaling pathways such as cell growth, cell cycle, cell motility, transcription, and apoptosis. Notably up-regulated genes are the cell cycle regulators E2F2 and Arl2. In accordance with a function of NSD3L in cell cycle regulation NSD3L depletion resulted in an increase in the number of cells in the S and G2/M cell cycle phases. Moreover, NSD3L depletion increased the invasiveness of MDA-MB-231 breast cancer cells indicating that NSD3L normally restrain cellular metastatic potential. Together the presented data indicates that NSD3L is a candidate tumor suppressor.",

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author = "Zhangle Zhou and Rune Thomsen and S{\o}ren Kahns and Nielsen, {Anders Lade} and Nielsen, {Anders Lade}",

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The NSD3L histone methyltransferase regulates cell cycle and cell invasion in breast cancer cells. / Zhou, Zhangle; Thomsen, Rune; Kahns, Søren et al.
I: Biochemical and Biophysical Research Communications, Bind 398, Nr. 3, 30.07.2010, s. 565-570.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - The NSD3L histone methyltransferase regulates cell cycle and cell invasion in breast cancer cells

AU - Zhou, Zhangle

AU - Thomsen, Rune

AU - Kahns, Søren

AU - Nielsen, Anders Lade

PY - 2010/7/30

Y1 - 2010/7/30

N2 - NSD3/WHSC1L1 histone methyltransferase gene aberrations are observed in leukemia and in breast and lung carcinomas, suggesting that NSD3 is implicated in carcinogenesis. In this study we examined in human breast cancer cells the NSD3L isoform which contains the catalytic histone methyltransferase SET-domain. siRNA directed depletion of NSD3L followed by genome-wide microarray analysis identified NSD3L regulated genes which could be functionally linked to cellular signaling pathways such as cell growth, cell cycle, cell motility, transcription, and apoptosis. Notably up-regulated genes are the cell cycle regulators E2F2 and Arl2. In accordance with a function of NSD3L in cell cycle regulation NSD3L depletion resulted in an increase in the number of cells in the S and G2/M cell cycle phases. Moreover, NSD3L depletion increased the invasiveness of MDA-MB-231 breast cancer cells indicating that NSD3L normally restrain cellular metastatic potential. Together the presented data indicates that NSD3L is a candidate tumor suppressor.

AB - NSD3/WHSC1L1 histone methyltransferase gene aberrations are observed in leukemia and in breast and lung carcinomas, suggesting that NSD3 is implicated in carcinogenesis. In this study we examined in human breast cancer cells the NSD3L isoform which contains the catalytic histone methyltransferase SET-domain. siRNA directed depletion of NSD3L followed by genome-wide microarray analysis identified NSD3L regulated genes which could be functionally linked to cellular signaling pathways such as cell growth, cell cycle, cell motility, transcription, and apoptosis. Notably up-regulated genes are the cell cycle regulators E2F2 and Arl2. In accordance with a function of NSD3L in cell cycle regulation NSD3L depletion resulted in an increase in the number of cells in the S and G2/M cell cycle phases. Moreover, NSD3L depletion increased the invasiveness of MDA-MB-231 breast cancer cells indicating that NSD3L normally restrain cellular metastatic potential. Together the presented data indicates that NSD3L is a candidate tumor suppressor.

KW - Breast Neoplasms

KW - Cell Cycle

KW - Cell Line, Tumor

KW - Female

KW - Gene Expression Regulation, Neoplastic

KW - Genome-Wide Association Study

KW - Histone-Lysine N-Methyltransferase

KW - Humans

KW - Neoplasm Invasiveness

KW - Nuclear Proteins

KW - Protein Isoforms

KW - RNA, Small Interfering

KW - Tumor Suppressor Proteins

U2 - 10.1016/j.bbrc.2010.06.119

DO - 10.1016/j.bbrc.2010.06.119

M3 - Journal article

C2 - 20599755

SN - 0006-291X

VL - 398

SP - 565

EP - 570

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

The NSD3L histone methyltransferase regulates cell cycle and cell invasion in breast cancer cells

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