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The Lotus japonicus NPF3.1 Is a Nodule-Induced Gene That Plays a Positive Role in Nodule Functioning

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Nitrogen-fixing nodules are new organs formed on legume roots as a result of the beneficial interaction with the soil bacteria, rhizobia. Proteins of the nitrate transporter 1/peptide transporter family (NPF) are largely represented in the subcategory of nodule-induced transporters identified in mature nodules. The role of nitrate as a signal/nutrient regulating nodule functioning has been recently highlighted in the literature, and NPFs may play a central role in both the permissive and inhibitory pathways controlling N2-fixation efficiency. In this study, we present the characterization of the Lotus japonicus LjNPF3.1 gene. LjNPF3.1 is upregulated in mature nodules. Promoter studies show transcriptional activation confined to the cortical region of both roots and nodules. Under symbiotic conditions, Ljnpf3.1-knockout mutant’s display reduced shoot development and anthocyanin accumulation as a result of nutrient deprivation. Altogether, LjNPF3.1 plays a role in maximizing the beneficial outcome of the root nodule symbiosis.

OriginalsprogEngelsk
Artikelnummer688187
TidsskriftFrontiers in Plant Science
Vol/bind12
ISSN1664-462X
DOI
StatusUdgivet - jun. 2021

Bibliografisk note

Funding Information:
We thank Marco Petruzziello and Giuseppina Zampi for technical assistance and Finn Pedersen for professional plant care. Funding. This work was supported by CNR Project (no.FOE-2019 DBA.AD003.139) and the European Community, Horizon 2020, Partnership for Research and Innovation in the Mediterranean Area, PRIMA, Section II, Legumes in biodiversity-based farming systems in Mediterranean Basin, LEGU-MED. YV was granted an Erasmus+ traineeship for graduate students from the University Federico II of Naples.

Funding Information:
This work was supported by CNR Project (no.FOE-2019 DBA. AD003.139) and the European Community, Horizon 2020, Partnership for Research and Innovation in the Mediterranean Area, PRIMA, Section II, Legumes in biodiversity-based farming systems in Mediterranean Basin, LEGU-MED. YV was granted an Erasmus+ traineeship for graduate students from the University Federico II of Naples.

Publisher Copyright:
© Copyright © 2021 Vittozzi, Nadzieja, Rogato, Radutoiu, Valkov and Chiurazzi.

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