TY - JOUR
T1 - The changing face of SDS denaturation
T2 - Complexes of Thermomyces lanuginosus lipase with SDS at pH 4.0, 6.0 and 8.0
AU - Rasmussen, Helena Østergaard
AU - Wollenberg, Daniel T Weltz
AU - Wang, Huabing
AU - Andersen, Kell K
AU - Oliveira, Cristiano L P
AU - Jørgensen, Christian Isak
AU - Jørgensen, Thomas J D
AU - Otzen, Daniel E
AU - Pedersen, Jan Skov
N1 - Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
PY - 2022/5
Y1 - 2022/5
N2 - HYPOTHESIS: Lipases are widely used in the detergent industry and must withstand harsh conditions involving both anionic and zwitterionic surfactants at alkaline pH. Thermomyces lanuginosus lipase (TlL) is often used and stays active at high concentrations of the anionic surfactant sodium dodecyl sulfate (SDS) at pH 8.0, but is sensitive to SDS at pH 6.0 and below. We propose that enhanced stability at pH 8.0 results from a structurally distinct complex formation with SDS.EXPERIMENTS: We use small-angle X-ray scattering (SAXS) to elucidate structures of TlL:SDS at pH 4.0, 6.0, and 8.0 and further investigate the complexes at pH 8.0 using hydrogen/deuterium exchange mass spectrometry (HDX-MS).FINDINGS: At pH 4.0, large dense aggregates are formed at low [SDS], which become gradually less dense at higher [SDS], resulting in a core-shell structure. At pH 6.0, SDS induces a TlL dimer and forms a hemi-micelle along the side of the dimer. At higher [SDS], TlL adopts a core-shell structure. At pH 8.0, TlL forms a dimer with a SDS hemi-micelle but avoids a core-shell structure and maintains activity. Three helices are identified as SDS anchor points. This study provides important structural insight into the stability of TlL towards SDS under alkaline conditions.
AB - HYPOTHESIS: Lipases are widely used in the detergent industry and must withstand harsh conditions involving both anionic and zwitterionic surfactants at alkaline pH. Thermomyces lanuginosus lipase (TlL) is often used and stays active at high concentrations of the anionic surfactant sodium dodecyl sulfate (SDS) at pH 8.0, but is sensitive to SDS at pH 6.0 and below. We propose that enhanced stability at pH 8.0 results from a structurally distinct complex formation with SDS.EXPERIMENTS: We use small-angle X-ray scattering (SAXS) to elucidate structures of TlL:SDS at pH 4.0, 6.0, and 8.0 and further investigate the complexes at pH 8.0 using hydrogen/deuterium exchange mass spectrometry (HDX-MS).FINDINGS: At pH 4.0, large dense aggregates are formed at low [SDS], which become gradually less dense at higher [SDS], resulting in a core-shell structure. At pH 6.0, SDS induces a TlL dimer and forms a hemi-micelle along the side of the dimer. At higher [SDS], TlL adopts a core-shell structure. At pH 8.0, TlL forms a dimer with a SDS hemi-micelle but avoids a core-shell structure and maintains activity. Three helices are identified as SDS anchor points. This study provides important structural insight into the stability of TlL towards SDS under alkaline conditions.
KW - Denaturation
KW - HDX-MS
KW - Lipase
KW - SAXS
KW - SDS
KW - Eurotiales/enzymology
KW - X-Ray Diffraction
KW - Ascomycota/chemistry
KW - Lipase/chemistry
KW - Scattering, Small Angle
KW - Hydrogen-Ion Concentration
U2 - 10.1016/j.jcis.2021.12.188
DO - 10.1016/j.jcis.2021.12.188
M3 - Journal article
C2 - 35092895
SN - 0021-9797
VL - 614
SP - 214
EP - 232
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
ER -