The Acute Response of Pericytes to Muscle-Damaging Eccentric Contraction and Protein Supplementation in Human Skeletal Muscle

Michael De Lisio; Jean Farup; Richard A Sukiennik; Nicole Clevenger; Julian Nallabelli; Brett Nelson; Kelly Ryan; Stine Klejs Rahbek; Frank de Paoli; Kristian Vissing; Marni D Boppart

doi:10.1152/japplphysiol.01112.2014

The Acute Response of Pericytes to Muscle-Damaging Eccentric Contraction and Protein Supplementation in Human Skeletal Muscle

Michael De Lisio, Jean Farup, Richard A Sukiennik, Nicole Clevenger, Julian Nallabelli, Brett Nelson, Kelly Ryan, Stine Klejs Rahbek, Frank de Paoli, Kristian Vissing, Marni D Boppart

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Abstract

Skeletal muscle pericytes increase in quantity following eccentric exercise (ECC) and contribute to myofiber repair and adaptation in mice. The purpose of the present investigation was to examine pericyte quantity in response to muscle-damaging ECC and protein supplementation in human skeletal muscle. Male subjects were divided into protein supplement (WHY; n=12) or isocaloric placebo (CHO; n=12) groups, and completed ECC using an isokinetic dynamometer. Supplements were consumed 3 times/day throughout the experimental time course. Biopsies were collected prior to (PRE) and 3, 24, 48, and 168 hours following ECC. Reflective of the damaging protocol, integrin subunits, including α7, β1A and β1D increased (3.8-fold, 3.6-fold and 3.9-fold, p<0.01) 24 hours post-ECC with no difference between supplements. Pericyte quantity did not change post-ECC. WHY resulted in a small, but significant decrease in ALP(+) pericytes when expressed as a percentage of myonuclei (CHO: 6.8 ± 0.3% vs. WHY: 5.8 ± 0.3%, p<0.05) or per myofiber (CHO: 0.119 ± 0.01 vs. WHY: 0.098 ± 0.01, p<0.05). The quantity of myonuclei expressing SRF, and the number of pericytes expressing SRF did not differ as a function of time post-ECC or supplement. These data demonstrate that acute muscle-damaging ECC increases α7β1 integrin content in human muscle, yet pericyte quantity is largely unaltered. Future studies should focus on the capacity for ECC to influence pericyte function, specifically paracrine factor release as a mechanism towards pericyte contribution to repair and adaptation post-exercise.

Originalsprog	Engelsk
Tidsskrift	Journal of Applied Physiology
Vol/bind	119
Nummer	8
Sider (fra-til)	900-907
Antal sider	8
ISSN	8750-7587
DOI	https://doi.org/10.1152/japplphysiol.01112.2014
Status	Udgivet - 15 okt. 2015

Adgang til dokumentet

10.1152/japplphysiol.01112.2014

Citationsformater

De Lisio, M., Farup, J., Sukiennik, R. A., Clevenger, N., Nallabelli, J., Nelson, B., Ryan, K., Rahbek, S. K., de Paoli, F., Vissing, K., & Boppart, M. D. (2015). The Acute Response of Pericytes to Muscle-Damaging Eccentric Contraction and Protein Supplementation in Human Skeletal Muscle. Journal of Applied Physiology, 119(8), 900-907. https://doi.org/10.1152/japplphysiol.01112.2014

@article{df46fcf9970e4c1eab1f2793b3a24142,

title = "The Acute Response of Pericytes to Muscle-Damaging Eccentric Contraction and Protein Supplementation in Human Skeletal Muscle",

abstract = "Skeletal muscle pericytes increase in quantity following eccentric exercise (ECC) and contribute to myofiber repair and adaptation in mice. The purpose of the present investigation was to examine pericyte quantity in response to muscle-damaging ECC and protein supplementation in human skeletal muscle. Male subjects were divided into protein supplement (WHY; n=12) or isocaloric placebo (CHO; n=12) groups, and completed ECC using an isokinetic dynamometer. Supplements were consumed 3 times/day throughout the experimental time course. Biopsies were collected prior to (PRE) and 3, 24, 48, and 168 hours following ECC. Reflective of the damaging protocol, integrin subunits, including α7, β1A and β1D increased (3.8-fold, 3.6-fold and 3.9-fold, p<0.01) 24 hours post-ECC with no difference between supplements. Pericyte quantity did not change post-ECC. WHY resulted in a small, but significant decrease in ALP(+) pericytes when expressed as a percentage of myonuclei (CHO: 6.8 ± 0.3% vs. WHY: 5.8 ± 0.3%, p<0.05) or per myofiber (CHO: 0.119 ± 0.01 vs. WHY: 0.098 ± 0.01, p<0.05). The quantity of myonuclei expressing SRF, and the number of pericytes expressing SRF did not differ as a function of time post-ECC or supplement. These data demonstrate that acute muscle-damaging ECC increases α7β1 integrin content in human muscle, yet pericyte quantity is largely unaltered. Future studies should focus on the capacity for ECC to influence pericyte function, specifically paracrine factor release as a mechanism towards pericyte contribution to repair and adaptation post-exercise.",

keywords = "mesenchymal stem cell, muscle damage, muscle regeneration, whey",

author = "{De Lisio}, Michael and Jean Farup and Sukiennik, {Richard A} and Nicole Clevenger and Julian Nallabelli and Brett Nelson and Kelly Ryan and Rahbek, {Stine Klejs} and {de Paoli}, Frank and Kristian Vissing and Boppart, {Marni D}",

note = "Copyright {\textcopyright} 2014, Journal of Applied Physiology.",

year = "2015",

month = oct,

day = "15",

doi = "10.1152/japplphysiol.01112.2014",

language = "English",

volume = "119",

pages = "900--907",

journal = "Journal of Applied Physiology",

issn = "8750-7587",

publisher = "AMER PHYSIOLOGICAL SOC",

number = "8",

}

De Lisio, M, Farup, J, Sukiennik, RA, Clevenger, N, Nallabelli, J, Nelson, B, Ryan, K, Rahbek, SK, de Paoli, F , Vissing, K & Boppart, MD 2015, 'The Acute Response of Pericytes to Muscle-Damaging Eccentric Contraction and Protein Supplementation in Human Skeletal Muscle', Journal of Applied Physiology, bind 119, nr. 8, s. 900-907. https://doi.org/10.1152/japplphysiol.01112.2014

The Acute Response of Pericytes to Muscle-Damaging Eccentric Contraction and Protein Supplementation in Human Skeletal Muscle. / De Lisio, Michael; Farup, Jean; Sukiennik, Richard A et al.
I: Journal of Applied Physiology, Bind 119, Nr. 8, 15.10.2015, s. 900-907.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - The Acute Response of Pericytes to Muscle-Damaging Eccentric Contraction and Protein Supplementation in Human Skeletal Muscle

AU - De Lisio, Michael

AU - Farup, Jean

AU - Sukiennik, Richard A

AU - Clevenger, Nicole

AU - Nallabelli, Julian

AU - Nelson, Brett

AU - Ryan, Kelly

AU - Rahbek, Stine Klejs

AU - de Paoli, Frank

AU - Vissing, Kristian

AU - Boppart, Marni D

PY - 2015/10/15

Y1 - 2015/10/15

N2 - Skeletal muscle pericytes increase in quantity following eccentric exercise (ECC) and contribute to myofiber repair and adaptation in mice. The purpose of the present investigation was to examine pericyte quantity in response to muscle-damaging ECC and protein supplementation in human skeletal muscle. Male subjects were divided into protein supplement (WHY; n=12) or isocaloric placebo (CHO; n=12) groups, and completed ECC using an isokinetic dynamometer. Supplements were consumed 3 times/day throughout the experimental time course. Biopsies were collected prior to (PRE) and 3, 24, 48, and 168 hours following ECC. Reflective of the damaging protocol, integrin subunits, including α7, β1A and β1D increased (3.8-fold, 3.6-fold and 3.9-fold, p<0.01) 24 hours post-ECC with no difference between supplements. Pericyte quantity did not change post-ECC. WHY resulted in a small, but significant decrease in ALP(+) pericytes when expressed as a percentage of myonuclei (CHO: 6.8 ± 0.3% vs. WHY: 5.8 ± 0.3%, p<0.05) or per myofiber (CHO: 0.119 ± 0.01 vs. WHY: 0.098 ± 0.01, p<0.05). The quantity of myonuclei expressing SRF, and the number of pericytes expressing SRF did not differ as a function of time post-ECC or supplement. These data demonstrate that acute muscle-damaging ECC increases α7β1 integrin content in human muscle, yet pericyte quantity is largely unaltered. Future studies should focus on the capacity for ECC to influence pericyte function, specifically paracrine factor release as a mechanism towards pericyte contribution to repair and adaptation post-exercise.

AB - Skeletal muscle pericytes increase in quantity following eccentric exercise (ECC) and contribute to myofiber repair and adaptation in mice. The purpose of the present investigation was to examine pericyte quantity in response to muscle-damaging ECC and protein supplementation in human skeletal muscle. Male subjects were divided into protein supplement (WHY; n=12) or isocaloric placebo (CHO; n=12) groups, and completed ECC using an isokinetic dynamometer. Supplements were consumed 3 times/day throughout the experimental time course. Biopsies were collected prior to (PRE) and 3, 24, 48, and 168 hours following ECC. Reflective of the damaging protocol, integrin subunits, including α7, β1A and β1D increased (3.8-fold, 3.6-fold and 3.9-fold, p<0.01) 24 hours post-ECC with no difference between supplements. Pericyte quantity did not change post-ECC. WHY resulted in a small, but significant decrease in ALP(+) pericytes when expressed as a percentage of myonuclei (CHO: 6.8 ± 0.3% vs. WHY: 5.8 ± 0.3%, p<0.05) or per myofiber (CHO: 0.119 ± 0.01 vs. WHY: 0.098 ± 0.01, p<0.05). The quantity of myonuclei expressing SRF, and the number of pericytes expressing SRF did not differ as a function of time post-ECC or supplement. These data demonstrate that acute muscle-damaging ECC increases α7β1 integrin content in human muscle, yet pericyte quantity is largely unaltered. Future studies should focus on the capacity for ECC to influence pericyte function, specifically paracrine factor release as a mechanism towards pericyte contribution to repair and adaptation post-exercise.

KW - mesenchymal stem cell

KW - muscle damage

KW - muscle regeneration

KW - whey

U2 - 10.1152/japplphysiol.01112.2014

DO - 10.1152/japplphysiol.01112.2014

M3 - Journal article

C2 - 26205545

SN - 8750-7587

VL - 119

SP - 900

EP - 907

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

IS - 8

ER -

The Acute Response of Pericytes to Muscle-Damaging Eccentric Contraction and Protein Supplementation in Human Skeletal Muscle

Abstract

Adgang til dokumentet

Fingeraftryk

Citationsformater