The acute effects of growth hormone in adipose tissue is associated with suppression of antilipolytic signals

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The acute effects of growth hormone in adipose tissue is associated with suppression of antilipolytic signals. / Høyer, Katrine L; Høgild, Morten L; List, Edward O; Lee, Kevin Y; Kissinger, Emily; Sharma, Rita; Erik Magnusson, Nils; Puri, Vishwajeet; Kopchick, John J; Jørgensen, Jens O L; Jessen, Niels.

I: Physiological Reports, Bind 8, Nr. 3, e14373, 02.2020.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Høyer, KL, Høgild, ML, List, EO, Lee, KY, Kissinger, E, Sharma, R, Erik Magnusson, N, Puri, V, Kopchick, JJ, Jørgensen, JOL & Jessen, N 2020, 'The acute effects of growth hormone in adipose tissue is associated with suppression of antilipolytic signals', Physiological Reports, bind 8, nr. 3, e14373. https://doi.org/10.14814/phy2.14373

APA

Høyer, K. L., Høgild, M. L., List, E. O., Lee, K. Y., Kissinger, E., Sharma, R., Erik Magnusson, N., Puri, V., Kopchick, J. J., Jørgensen, J. O. L., & Jessen, N. (2020). The acute effects of growth hormone in adipose tissue is associated with suppression of antilipolytic signals. Physiological Reports, 8(3), [e14373]. https://doi.org/10.14814/phy2.14373

CBE

Høyer KL, Høgild ML, List EO, Lee KY, Kissinger E, Sharma R, Erik Magnusson N, Puri V, Kopchick JJ, Jørgensen JOL, Jessen N. 2020. The acute effects of growth hormone in adipose tissue is associated with suppression of antilipolytic signals. Physiological Reports. 8(3):Article e14373. https://doi.org/10.14814/phy2.14373

MLA

Vancouver

Author

Høyer, Katrine L ; Høgild, Morten L ; List, Edward O ; Lee, Kevin Y ; Kissinger, Emily ; Sharma, Rita ; Erik Magnusson, Nils ; Puri, Vishwajeet ; Kopchick, John J ; Jørgensen, Jens O L ; Jessen, Niels. / The acute effects of growth hormone in adipose tissue is associated with suppression of antilipolytic signals. I: Physiological Reports. 2020 ; Bind 8, Nr. 3.

Bibtex

@article{1f1a9d0424ae4114aedfc340370dfbed,
title = "The acute effects of growth hormone in adipose tissue is associated with suppression of antilipolytic signals",
abstract = "AIM: Since GH stimulates lipolysis in vivo after a 2-hr lag phase, we studied whether this involves GH signaling and gene expression in adipose tissue (AT).METHODS: Human subjects (n = 9) each underwent intravenous exposure to GH versus saline with measurement of serum FFA, and GH signaling, gene array, and protein in AT biopsies after 30-120 min. Human data were corroborated in adipose-specific GH receptor knockout (FaGHRKO) mice versus wild-type mice. Expression of candidate genes identified in the array were investigated in 3T3-L1 adipocytes.RESULTS: GH increased serum FFA and AT phosphorylation of STAT5b in human subjects. This was replicated in wild-type mice, but not in FaGHRKO mice. The array identified 53 GH-regulated genes, and Ingenuity Pathway analysis showed downregulation of PDE3b, an insulin-dependent antilipolytic signal, upregulation of PTEN that inhibits insulin-dependent antilipolysis, and downregulation of G0S2 and RASD1, both encoding antilipolytic proteins. This was confirmed in 3T3-L1 adipocytes, except for PDE3B, including reciprocal effects of GH and insulin on mRNA expression of PTEN, RASD1, and G0S2.CONCLUSION: (a) GH directly stimulates AT lipolysis in a GHR-dependent manner, (b) this involves suppression of antilipolytic signals at the level of gene expression, (c) the underlying GH signaling pathways remain to be defined.",
author = "H{\o}yer, {Katrine L} and H{\o}gild, {Morten L} and List, {Edward O} and Lee, {Kevin Y} and Emily Kissinger and Rita Sharma and {Erik Magnusson}, Nils and Vishwajeet Puri and Kopchick, {John J} and J{\o}rgensen, {Jens O L} and Niels Jessen",
note = "{\textcopyright} 2020 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.",
year = "2020",
month = feb,
doi = "10.14814/phy2.14373",
language = "English",
volume = "8",
journal = "Physiological Reports",
issn = "2051-817X",
publisher = "Wiley Periodicals, Inc.",
number = "3",

}

RIS

TY - JOUR

T1 - The acute effects of growth hormone in adipose tissue is associated with suppression of antilipolytic signals

AU - Høyer, Katrine L

AU - Høgild, Morten L

AU - List, Edward O

AU - Lee, Kevin Y

AU - Kissinger, Emily

AU - Sharma, Rita

AU - Erik Magnusson, Nils

AU - Puri, Vishwajeet

AU - Kopchick, John J

AU - Jørgensen, Jens O L

AU - Jessen, Niels

N1 - © 2020 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

PY - 2020/2

Y1 - 2020/2

N2 - AIM: Since GH stimulates lipolysis in vivo after a 2-hr lag phase, we studied whether this involves GH signaling and gene expression in adipose tissue (AT).METHODS: Human subjects (n = 9) each underwent intravenous exposure to GH versus saline with measurement of serum FFA, and GH signaling, gene array, and protein in AT biopsies after 30-120 min. Human data were corroborated in adipose-specific GH receptor knockout (FaGHRKO) mice versus wild-type mice. Expression of candidate genes identified in the array were investigated in 3T3-L1 adipocytes.RESULTS: GH increased serum FFA and AT phosphorylation of STAT5b in human subjects. This was replicated in wild-type mice, but not in FaGHRKO mice. The array identified 53 GH-regulated genes, and Ingenuity Pathway analysis showed downregulation of PDE3b, an insulin-dependent antilipolytic signal, upregulation of PTEN that inhibits insulin-dependent antilipolysis, and downregulation of G0S2 and RASD1, both encoding antilipolytic proteins. This was confirmed in 3T3-L1 adipocytes, except for PDE3B, including reciprocal effects of GH and insulin on mRNA expression of PTEN, RASD1, and G0S2.CONCLUSION: (a) GH directly stimulates AT lipolysis in a GHR-dependent manner, (b) this involves suppression of antilipolytic signals at the level of gene expression, (c) the underlying GH signaling pathways remain to be defined.

AB - AIM: Since GH stimulates lipolysis in vivo after a 2-hr lag phase, we studied whether this involves GH signaling and gene expression in adipose tissue (AT).METHODS: Human subjects (n = 9) each underwent intravenous exposure to GH versus saline with measurement of serum FFA, and GH signaling, gene array, and protein in AT biopsies after 30-120 min. Human data were corroborated in adipose-specific GH receptor knockout (FaGHRKO) mice versus wild-type mice. Expression of candidate genes identified in the array were investigated in 3T3-L1 adipocytes.RESULTS: GH increased serum FFA and AT phosphorylation of STAT5b in human subjects. This was replicated in wild-type mice, but not in FaGHRKO mice. The array identified 53 GH-regulated genes, and Ingenuity Pathway analysis showed downregulation of PDE3b, an insulin-dependent antilipolytic signal, upregulation of PTEN that inhibits insulin-dependent antilipolysis, and downregulation of G0S2 and RASD1, both encoding antilipolytic proteins. This was confirmed in 3T3-L1 adipocytes, except for PDE3B, including reciprocal effects of GH and insulin on mRNA expression of PTEN, RASD1, and G0S2.CONCLUSION: (a) GH directly stimulates AT lipolysis in a GHR-dependent manner, (b) this involves suppression of antilipolytic signals at the level of gene expression, (c) the underlying GH signaling pathways remain to be defined.

U2 - 10.14814/phy2.14373

DO - 10.14814/phy2.14373

M3 - Journal article

C2 - 32073221

VL - 8

JO - Physiological Reports

JF - Physiological Reports

SN - 2051-817X

IS - 3

M1 - e14373

ER -