Temperature tactics:: Targeting acetate or methane production in autotrophic H2/CO2 conversion with mixed cultures

Paola Andrea Palacios Jaramillo; Mads Ujarak Sieborg; Simon Borg Kuipers; Simon Fruergaard; Michael Vedel Wegener Kofoed

doi:10.1016/j.bej.2024.109574

Temperature tactics: Targeting acetate or methane production in autotrophic H2/CO2 conversion with mixed cultures

Paola Andrea Palacios Jaramillo, Mads Ujarak Sieborg, Simon Borg Kuipers, Simon Fruergaard, Michael Vedel Wegener Kofoed^*

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

4 Citationer (Scopus)

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Abstract

Ex situ biomethanation is a promising technology that combines the concepts of Power-to-X and carbon capture and utilization (CCU) by employing microorganisms to convert hydrogen (H₂) and carbon dioxide (CO₂) into biomethane (CH₄). Mesophilic (37°C) and thermophilic (50°C) ex situ biomethanation processes in continuous stirred tank reactors (CSTRs), were evaluated for the first time under a high continuous H₂/CO₂ supply. Each process was evaluated with and without the addition of minerals and vitamins. In all thermophilic reactors, methanogenesis was favored, achieving stable methane yields of 110 ± 8 %. Additionally, the addition of minerals resulted in a 67 % increase in H₂ consumption rates. In contrast, mesophilic reactors showed low CH₄ yields (7.5 %) and acetogenesis as the dominant pathway, with acetate concentrations reaching up to 7.6 ± 2 g L⁻¹. The effect of free acetic acid (FAA), a rarely considered concept based on the correlation between pH and acetic acid concentration, was also tested on thermophilic ex situ CSTRs. A clear negative correlation was demonstrated between FAA concentration and the CH₄ productivity, with a 50 % inhibition of CH₄ productivity at 0.062 g L⁻¹ of FAA.

Originalsprog	Engelsk
Artikelnummer	109574
Tidsskrift	Biochemical Engineering Journal
Vol/bind	214
Antal sider	10
ISSN	1369-703X
DOI	https://doi.org/10.1016/j.bej.2024.109574
Status	Udgivet - feb. 2025

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10.1016/j.bej.2024.109574Licens: CC BY

1-s2.0-S1369703X24003619-mainForlagets udgivne version, 2,38 MBLicens: CC BY

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@article{1a22ce461e764a1b9f6d8201c782494c,

title = "Temperature tactics:: Targeting acetate or methane production in autotrophic H2/CO2 conversion with mixed cultures",

abstract = "Ex situ biomethanation is a promising technology that combines the concepts of Power-to-X and carbon capture and utilization (CCU) by employing microorganisms to convert hydrogen (H2) and carbon dioxide (CO2) into biomethane (CH4). Mesophilic (37°C) and thermophilic (50°C) ex situ biomethanation processes in continuous stirred tank reactors (CSTRs), were evaluated for the first time under a high continuous H2/CO2 supply. Each process was evaluated with and without the addition of minerals and vitamins. In all thermophilic reactors, methanogenesis was favored, achieving stable methane yields of 110 ± 8 \%. Additionally, the addition of minerals resulted in a 67 \% increase in H2 consumption rates. In contrast, mesophilic reactors showed low CH4 yields (7.5 \%) and acetogenesis as the dominant pathway, with acetate concentrations reaching up to 7.6 ± 2 g L−1. The effect of free acetic acid (FAA), a rarely considered concept based on the correlation between pH and acetic acid concentration, was also tested on thermophilic ex situ CSTRs. A clear negative correlation was demonstrated between FAA concentration and the CH4 productivity, with a 50 \% inhibition of CH4 productivity at 0.062 g L−1 of FAA.",

keywords = "Acetogenesis, CO utilization, Ex situ biomethanation, Free acetic acid (FAA), Mesophilic, Methanogenesis, Thermophilic",

author = "\{Palacios Jaramillo\}, \{Paola Andrea\} and Sieborg, \{Mads Ujarak\} and Kuipers, \{Simon Borg\} and Simon Fruergaard and Kofoed, \{Michael Vedel Wegener\}",

year = "2025",

month = feb,

doi = "10.1016/j.bej.2024.109574",

language = "English",

volume = "214",

journal = "Biochemical Engineering Journal",

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publisher = "Elsevier B.V.",

}

Temperature tactics: Targeting acetate or methane production in autotrophic H2/CO2 conversion with mixed cultures. / Palacios Jaramillo, Paola Andrea ; Sieborg, Mads Ujarak; Kuipers, Simon Borg et al.
I: Biochemical Engineering Journal, Bind 214, 109574, 02.2025.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Temperature tactics:

T2 - Targeting acetate or methane production in autotrophic H2/CO2 conversion with mixed cultures

AU - Palacios Jaramillo, Paola Andrea

AU - Sieborg, Mads Ujarak

AU - Kuipers, Simon Borg

AU - Fruergaard, Simon

AU - Kofoed, Michael Vedel Wegener

PY - 2025/2

Y1 - 2025/2

N2 - Ex situ biomethanation is a promising technology that combines the concepts of Power-to-X and carbon capture and utilization (CCU) by employing microorganisms to convert hydrogen (H2) and carbon dioxide (CO2) into biomethane (CH4). Mesophilic (37°C) and thermophilic (50°C) ex situ biomethanation processes in continuous stirred tank reactors (CSTRs), were evaluated for the first time under a high continuous H2/CO2 supply. Each process was evaluated with and without the addition of minerals and vitamins. In all thermophilic reactors, methanogenesis was favored, achieving stable methane yields of 110 ± 8 %. Additionally, the addition of minerals resulted in a 67 % increase in H2 consumption rates. In contrast, mesophilic reactors showed low CH4 yields (7.5 %) and acetogenesis as the dominant pathway, with acetate concentrations reaching up to 7.6 ± 2 g L−1. The effect of free acetic acid (FAA), a rarely considered concept based on the correlation between pH and acetic acid concentration, was also tested on thermophilic ex situ CSTRs. A clear negative correlation was demonstrated between FAA concentration and the CH4 productivity, with a 50 % inhibition of CH4 productivity at 0.062 g L−1 of FAA.

AB - Ex situ biomethanation is a promising technology that combines the concepts of Power-to-X and carbon capture and utilization (CCU) by employing microorganisms to convert hydrogen (H2) and carbon dioxide (CO2) into biomethane (CH4). Mesophilic (37°C) and thermophilic (50°C) ex situ biomethanation processes in continuous stirred tank reactors (CSTRs), were evaluated for the first time under a high continuous H2/CO2 supply. Each process was evaluated with and without the addition of minerals and vitamins. In all thermophilic reactors, methanogenesis was favored, achieving stable methane yields of 110 ± 8 %. Additionally, the addition of minerals resulted in a 67 % increase in H2 consumption rates. In contrast, mesophilic reactors showed low CH4 yields (7.5 %) and acetogenesis as the dominant pathway, with acetate concentrations reaching up to 7.6 ± 2 g L−1. The effect of free acetic acid (FAA), a rarely considered concept based on the correlation between pH and acetic acid concentration, was also tested on thermophilic ex situ CSTRs. A clear negative correlation was demonstrated between FAA concentration and the CH4 productivity, with a 50 % inhibition of CH4 productivity at 0.062 g L−1 of FAA.

KW - Acetogenesis

KW - CO utilization

KW - Ex situ biomethanation

KW - Free acetic acid (FAA)

KW - Mesophilic

KW - Methanogenesis

KW - Thermophilic

UR - https://www.scopus.com/pages/publications/85209927923

U2 - 10.1016/j.bej.2024.109574

DO - 10.1016/j.bej.2024.109574

M3 - Journal article

SN - 1369-703X

VL - 214

JO - Biochemical Engineering Journal

JF - Biochemical Engineering Journal

M1 - 109574

ER -