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Temperature Sensitivity and Composition of Nitrate-Reducing Microbiomes from a Full-Scale Woodchip Bioreactor Treating Agricultural Drainage Water

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Temperature Sensitivity and Composition of Nitrate-Reducing Microbiomes from a Full-Scale Woodchip Bioreactor Treating Agricultural Drainage Water. / Jeglot, Arnaud; Sorensen, Sebastian Reinhold; Schnorr, Kirk M.; Plauborg, Finn; Elsgaard, Lars.

I: Microorganisms, Bind 9, Nr. 6, 1331, 06.2021.

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@article{e551a2db707b48edb2d7fd62c204cf45,
title = "Temperature Sensitivity and Composition of Nitrate-Reducing Microbiomes from a Full-Scale Woodchip Bioreactor Treating Agricultural Drainage Water",
abstract = "Denitrifying woodchip bioreactors (WBR), which aim to reduce nitrate (NO3-) pollution from agricultural drainage water, are less efficient when cold temperatures slow down the microbial transformation processes. Conducting bioaugmentation could potentially increase the NO3- removal efficiency during these specific periods. First, it is necessary to investigate denitrifying microbial populations in these facilities and understand their temperature responses. We hypothesized that seasonal changes and subsequent adaptations of microbial populations would allow for enrichment of cold-adapted denitrifying bacterial populations with potential use for bioaugmentation. Woodchip material was sampled from an operating WBR during spring, fall, and winter and used for enrichments of denitrifiers that were characterized by studies of metagenomics and temperature dependence of NO3- depletion. The successful enrichment of psychrotolerant denitrifiers was supported by the differences in temperature response, with the apparent domination of the phylum Proteobacteria and the genus Pseudomonas. The enrichments were found to have different microbiomes' composition and they mainly differed with native woodchip microbiomes by a lower abundance of the genus Flavobacterium. Overall, the performance and composition of the enriched denitrifying population from the WBR microbiome indicated a potential for efficient NO3- removal at cold temperatures that could be stimulated by the addition of selected cold-adapted denitrifying bacteria.",
keywords = "heterotrophic denitrification, metagenomics, psychrotolerant denitrifiers, nitrate removal, constructed wetland, FIELD DENITRIFICATION BEDS, NITROUS-OXIDE PRODUCTION, DENITRIFYING BACTERIA, FUNCTIONAL GENES, REMOVAL, COMMUNITIES, REDUCTION, KINETICS, MARINE, CARBON",
author = "Arnaud Jeglot and Sorensen, {Sebastian Reinhold} and Schnorr, {Kirk M.} and Finn Plauborg and Lars Elsgaard",
year = "2021",
month = jun,
doi = "10.3390/microorganisms9061331",
language = "English",
volume = "9",
journal = "Microorganisms",
issn = "2076-2607",
publisher = "M D P I AG",
number = "6",

}

RIS

TY - JOUR

T1 - Temperature Sensitivity and Composition of Nitrate-Reducing Microbiomes from a Full-Scale Woodchip Bioreactor Treating Agricultural Drainage Water

AU - Jeglot, Arnaud

AU - Sorensen, Sebastian Reinhold

AU - Schnorr, Kirk M.

AU - Plauborg, Finn

AU - Elsgaard, Lars

PY - 2021/6

Y1 - 2021/6

N2 - Denitrifying woodchip bioreactors (WBR), which aim to reduce nitrate (NO3-) pollution from agricultural drainage water, are less efficient when cold temperatures slow down the microbial transformation processes. Conducting bioaugmentation could potentially increase the NO3- removal efficiency during these specific periods. First, it is necessary to investigate denitrifying microbial populations in these facilities and understand their temperature responses. We hypothesized that seasonal changes and subsequent adaptations of microbial populations would allow for enrichment of cold-adapted denitrifying bacterial populations with potential use for bioaugmentation. Woodchip material was sampled from an operating WBR during spring, fall, and winter and used for enrichments of denitrifiers that were characterized by studies of metagenomics and temperature dependence of NO3- depletion. The successful enrichment of psychrotolerant denitrifiers was supported by the differences in temperature response, with the apparent domination of the phylum Proteobacteria and the genus Pseudomonas. The enrichments were found to have different microbiomes' composition and they mainly differed with native woodchip microbiomes by a lower abundance of the genus Flavobacterium. Overall, the performance and composition of the enriched denitrifying population from the WBR microbiome indicated a potential for efficient NO3- removal at cold temperatures that could be stimulated by the addition of selected cold-adapted denitrifying bacteria.

AB - Denitrifying woodchip bioreactors (WBR), which aim to reduce nitrate (NO3-) pollution from agricultural drainage water, are less efficient when cold temperatures slow down the microbial transformation processes. Conducting bioaugmentation could potentially increase the NO3- removal efficiency during these specific periods. First, it is necessary to investigate denitrifying microbial populations in these facilities and understand their temperature responses. We hypothesized that seasonal changes and subsequent adaptations of microbial populations would allow for enrichment of cold-adapted denitrifying bacterial populations with potential use for bioaugmentation. Woodchip material was sampled from an operating WBR during spring, fall, and winter and used for enrichments of denitrifiers that were characterized by studies of metagenomics and temperature dependence of NO3- depletion. The successful enrichment of psychrotolerant denitrifiers was supported by the differences in temperature response, with the apparent domination of the phylum Proteobacteria and the genus Pseudomonas. The enrichments were found to have different microbiomes' composition and they mainly differed with native woodchip microbiomes by a lower abundance of the genus Flavobacterium. Overall, the performance and composition of the enriched denitrifying population from the WBR microbiome indicated a potential for efficient NO3- removal at cold temperatures that could be stimulated by the addition of selected cold-adapted denitrifying bacteria.

KW - heterotrophic denitrification

KW - metagenomics

KW - psychrotolerant denitrifiers

KW - nitrate removal

KW - constructed wetland

KW - FIELD DENITRIFICATION BEDS

KW - NITROUS-OXIDE PRODUCTION

KW - DENITRIFYING BACTERIA

KW - FUNCTIONAL GENES

KW - REMOVAL

KW - COMMUNITIES

KW - REDUCTION

KW - KINETICS

KW - MARINE

KW - CARBON

U2 - 10.3390/microorganisms9061331

DO - 10.3390/microorganisms9061331

M3 - Journal article

C2 - 34207422

VL - 9

JO - Microorganisms

JF - Microorganisms

SN - 2076-2607

IS - 6

M1 - 1331

ER -