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Targeted proteomics as a tool for porcine acute phase proteins measurements

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  • Anna Marco-Ramell, Dept. Bioquímica, Universitat Autònoma de Barcelona , Spanien
  • Anna Bassols, Dept. Bioquímica, Universitat Autònoma de Barcelona , Spanien
  • Stine Lønnerup Bislev, Danmark
  • Kristian Wejse Sanggaard, Danmark
  • Jan Johannes Enghild
  • Emøke Bendixen
  • Lorenzo Fraile, 3Centre de Recerca en Sanitat Animal , Spanien
It is mandatory, after protein identification, to validate candidate markers in a larger amount of samples for biomarker discovery. The most used methods for validation of new markers are enzymatic or immunoassay-based commercial kits. However, relatively few kits are available for farm animals. Selected reaction monitoring (SRM), a targeted quantitative proteomic technique, may be used as an alternative to commercial kits for the measurement and validation of target proteins. Acute phase proteins (APPs) are widely recognized inflammation and infection biomarkers (Eckersall, 2010) and there is recent evidence that they can be also considered as welfare markers (Giannetto et al., 2011). During an acute stress situation, the levels of some interleukins (IL-1β, IL-6 and TNF-α) are increased (Elenkov and Chrousos, 2002) and consequently the concentration of the APPs varies dramatically. In pigs the most important APPs include haptoglobin (Hp), C-reactive protein (CRP), the inter-α-inhibitor-heavy chain 4 (ITIH4, also called Pig major acute phase protein, or Pig-MAP), serum amyloid A (SAA) and apolipoprotein A-I (Apo A-I), but also other proteins are well known to mark the acute phase response, as albumin, transferrin (Heegaard et al., 2011) and fetuin A (Brown et al., 1992).
TitelProceedings of the 4th Management Committee Meeting and 3rd Meeting of Working Groups 1, 2 & 3 of COST Action FA1002
RedaktørerAndré de Almeida , David Eckersall , Elena Bencurova , Saskia Dolinska, Patrik Mlynarcik , Miroslava Vincova , Mangesh Bhide
Antal sider4
ForlagWageningen Academic Publishers
Udgivelsesår25 apr. 2013
ISBN (Elektronisk)978-90-8686-776-9
StatusUdgivet - 25 apr. 2013
BegivenhedFarm animal proteomics 2013 - Košice, Slovakiet
Varighed: 25 apr. 201326 apr. 2013
Konferencens nummer: 4th


KonferenceFarm animal proteomics 2013

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