Synthetic Translational Regulation by Protein-Binding RNA Origami Scaffolds

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisLetterpeer review

12 Downloads (Pure)

Abstract

Rational design approaches for the regulation of gene expression are expanding the synthetic biology toolbox. However, only a few tools for regulating gene expression at the translational level have been developed. Here, we devise an approach for translational regulation using the MS2 and PP7 aptamer and coat-protein pairs in Escherichia coli. The aptamers are used as operators in transcription units that encode proteins fused to their cognate coat proteins, which leads to self-repression. RNA origami scaffolds that contain up to four aptamers serve as an alternate binder to activate translation. With this system, we demonstrate that the increase in expression of a reporter protein is dependent on both the concentration and number of aptamers on RNA origami scaffolds. We also demonstrate regulation of multiple proteins using a single MS2 coat protein fusion and apply this method to regulate the relative expression of enzymes of the branched pathway for deoxyviolacein biosynthesis.

OriginalsprogEngelsk
TidsskriftA C S Synthetic Biology
Vol/bind11
Nummer5
Sider (fra-til)1710-1718
Antal sider9
ISSN2161-5063
DOI
StatusUdgivet - 20 maj 2022

Fingeraftryk

Dyk ned i forskningsemnerne om 'Synthetic Translational Regulation by Protein-Binding RNA Origami Scaffolds'. Sammen danner de et unikt fingeraftryk.

Citationsformater