Aarhus University Seal / Aarhus Universitets segl

Synthesis of Dopamine and Serotonin Derivatives for Immobilization on a Solid Support

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • Erik Daa Funder, Danmark
  • Anne Bjørnskov Jensen
  • ,
  • Thomas Tørring, Danmark
  • Anne Louise Bank Kodal, Danmark
  • Ane Rebolledo Azcargorta
  • ,
  • Kurt Vesterager Gothelf
The two important neurotransmitters dopamine and serotonin are synthesized with short PEG tethers and immobilized on a magnetic solid support. The tether is attached to the aromatic moiety of the neurotransmitters to conserve their original functional groups. This approach causes minimal alteration of the original structure with the aim of optimizing the immobilized neurotransmitters for aptamer selection by SELEX. For the dopamine derivative, the tether is attached to the aromatic core of a dopamine precursor by the Sonogashira reaction. For serotonin, a link to the indole core is introduced by a Claisen rearrangement from the allylated phenol moiety of serotonin. The tethers are azide-functionalized, which enables coupling to alkyne-modified magnetic beads. The coupling to the magnetic beads is quantified by UV spectroscopy using Fmoc-monitoring of the immobilized dopamine and serotonin derivatives.
TidsskriftJournal of Organic Chemistry
Sider (fra-til)3134-3142
Antal sider9
StatusUdgivet - 5 mar. 2012

Se relationer på Aarhus Universitet Citationsformater

ID: 52230407