Aarhus University Seal / Aarhus Universitets segl

Synthesis, Dynamic Combinatorial Chemistry, and PCR Amplification of 3'-5' and 3'-6' Disulfide-linked Oligonucleotides

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Synthesis, Dynamic Combinatorial Chemistry, and PCR Amplification of 3'-5' and 3'-6' Disulfide-linked Oligonucleotides. / Hansen, Dennis Jul; Manuguerra, Ilenia; Kjelstrup, Michael Brøndum; Gothelf, Kurt Vesterager.

I: Angewandte Chemie International Edition, Bind 53, Nr. 52, 20.11.2014, s. 14415-14418.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Hansen, DJ, Manuguerra, I, Kjelstrup, MB & Gothelf, KV 2014, 'Synthesis, Dynamic Combinatorial Chemistry, and PCR Amplification of 3'-5' and 3'-6' Disulfide-linked Oligonucleotides', Angewandte Chemie International Edition, bind 53, nr. 52, s. 14415-14418. https://doi.org/10.1002/anie.201405761

APA

Hansen, D. J., Manuguerra, I., Kjelstrup, M. B., & Gothelf, K. V. (2014). Synthesis, Dynamic Combinatorial Chemistry, and PCR Amplification of 3'-5' and 3'-6' Disulfide-linked Oligonucleotides. Angewandte Chemie International Edition, 53(52), 14415-14418. https://doi.org/10.1002/anie.201405761

CBE

MLA

Vancouver

Hansen DJ, Manuguerra I, Kjelstrup MB, Gothelf KV. Synthesis, Dynamic Combinatorial Chemistry, and PCR Amplification of 3'-5' and 3'-6' Disulfide-linked Oligonucleotides. Angewandte Chemie International Edition. 2014 nov 20;53(52):14415-14418. https://doi.org/10.1002/anie.201405761

Author

Hansen, Dennis Jul ; Manuguerra, Ilenia ; Kjelstrup, Michael Brøndum ; Gothelf, Kurt Vesterager. / Synthesis, Dynamic Combinatorial Chemistry, and PCR Amplification of 3'-5' and 3'-6' Disulfide-linked Oligonucleotides. I: Angewandte Chemie International Edition. 2014 ; Bind 53, Nr. 52. s. 14415-14418.

Bibtex

@article{cf09aa1dac5542a3863be0fc8ad1bda6,
title = "Synthesis, Dynamic Combinatorial Chemistry, and PCR Amplification of 3'-5' and 3'-6' Disulfide-linked Oligonucleotides",
abstract = "Disulfide dithymidines linked 3'-5' or 3'-6' were synthesized and incorporated into oligonucleotides through a combined phosphotriester and phosphoramidite solid-phase oligonucleotide synthesis approach. The disulfide links are cleaved and formed reversibly in the presence of thiols and oligonucleotides. This link was shown to be sequence-adaptive in response to given templates in the presence of mercaptoethanol. The artificial 3'-5' and 3'-6' disulfide link was tolerated by polymerases in the polymerase chain reaction (PCR). By using sequencing analysis, we show that single mutations frequently occurred randomly in the amplification products of the PCR.",
author = "Hansen, {Dennis Jul} and Ilenia Manuguerra and Kjelstrup, {Michael Br{\o}ndum} and Gothelf, {Kurt Vesterager}",
year = "2014",
month = nov,
day = "20",
doi = "10.1002/anie.201405761",
language = "English",
volume = "53",
pages = "14415--14418",
journal = "Angewandte Chemie International Edition",
issn = "1433-7851",
publisher = "Wiley-VCH",
number = "52",

}

RIS

TY - JOUR

T1 - Synthesis, Dynamic Combinatorial Chemistry, and PCR Amplification of 3'-5' and 3'-6' Disulfide-linked Oligonucleotides

AU - Hansen, Dennis Jul

AU - Manuguerra, Ilenia

AU - Kjelstrup, Michael Brøndum

AU - Gothelf, Kurt Vesterager

PY - 2014/11/20

Y1 - 2014/11/20

N2 - Disulfide dithymidines linked 3'-5' or 3'-6' were synthesized and incorporated into oligonucleotides through a combined phosphotriester and phosphoramidite solid-phase oligonucleotide synthesis approach. The disulfide links are cleaved and formed reversibly in the presence of thiols and oligonucleotides. This link was shown to be sequence-adaptive in response to given templates in the presence of mercaptoethanol. The artificial 3'-5' and 3'-6' disulfide link was tolerated by polymerases in the polymerase chain reaction (PCR). By using sequencing analysis, we show that single mutations frequently occurred randomly in the amplification products of the PCR.

AB - Disulfide dithymidines linked 3'-5' or 3'-6' were synthesized and incorporated into oligonucleotides through a combined phosphotriester and phosphoramidite solid-phase oligonucleotide synthesis approach. The disulfide links are cleaved and formed reversibly in the presence of thiols and oligonucleotides. This link was shown to be sequence-adaptive in response to given templates in the presence of mercaptoethanol. The artificial 3'-5' and 3'-6' disulfide link was tolerated by polymerases in the polymerase chain reaction (PCR). By using sequencing analysis, we show that single mutations frequently occurred randomly in the amplification products of the PCR.

U2 - 10.1002/anie.201405761

DO - 10.1002/anie.201405761

M3 - Journal article

C2 - 25413927

VL - 53

SP - 14415

EP - 14418

JO - Angewandte Chemie International Edition

JF - Angewandte Chemie International Edition

SN - 1433-7851

IS - 52

ER -