Substrate-Dependent Fermentation of Bamboo in Giant Panda Gut Microbiomes: Leaf Primarily to Ethanol and Pith to Lactate

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Substrate-Dependent Fermentation of Bamboo in Giant Panda Gut Microbiomes : Leaf Primarily to Ethanol and Pith to Lactate. / Scoma, Alberto; Khor, Way Cern; Coma, Marta; Heyer, Robert; Props, Ruben; Schoelynck, Jonas; Bouts, Tim; Benndorf, Dirk; Li, Desheng; Zhang, Hemin; Rabaey, Korneel.

I: Frontiers in Microbiology, Bind 11, 530, 2020.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Scoma, A, Khor, WC, Coma, M, Heyer, R, Props, R, Schoelynck, J, Bouts, T, Benndorf, D, Li, D, Zhang, H & Rabaey, K 2020, 'Substrate-Dependent Fermentation of Bamboo in Giant Panda Gut Microbiomes: Leaf Primarily to Ethanol and Pith to Lactate', Frontiers in Microbiology, bind 11, 530. https://doi.org/10.3389/fmicb.2020.00530

APA

Scoma, A., Khor, W. C., Coma, M., Heyer, R., Props, R., Schoelynck, J., Bouts, T., Benndorf, D., Li, D., Zhang, H., & Rabaey, K. (2020). Substrate-Dependent Fermentation of Bamboo in Giant Panda Gut Microbiomes: Leaf Primarily to Ethanol and Pith to Lactate. Frontiers in Microbiology, 11, [530]. https://doi.org/10.3389/fmicb.2020.00530

CBE

Scoma A, Khor WC, Coma M, Heyer R, Props R, Schoelynck J, Bouts T, Benndorf D, Li D, Zhang H, Rabaey K. 2020. Substrate-Dependent Fermentation of Bamboo in Giant Panda Gut Microbiomes: Leaf Primarily to Ethanol and Pith to Lactate. Frontiers in Microbiology. 11:Article 530. https://doi.org/10.3389/fmicb.2020.00530

MLA

Vancouver

Author

Scoma, Alberto ; Khor, Way Cern ; Coma, Marta ; Heyer, Robert ; Props, Ruben ; Schoelynck, Jonas ; Bouts, Tim ; Benndorf, Dirk ; Li, Desheng ; Zhang, Hemin ; Rabaey, Korneel. / Substrate-Dependent Fermentation of Bamboo in Giant Panda Gut Microbiomes : Leaf Primarily to Ethanol and Pith to Lactate. I: Frontiers in Microbiology. 2020 ; Bind 11.

Bibtex

@article{c91f476135184d598ddbf651b409cba2,
title = "Substrate-Dependent Fermentation of Bamboo in Giant Panda Gut Microbiomes: Leaf Primarily to Ethanol and Pith to Lactate",
abstract = "The giant panda is known worldwide for having successfully moved to a diet almost exclusively based on bamboo. Provided that no lignocellulose-degrading enzyme was detected in panda{\textquoteright}s genome, bamboo digestion is believed to depend on its gut microbiome. However, pandas retain the digestive system of a carnivore, with retention times of maximum 12 h. Cultivation of their unique gut microbiome under controlled laboratory conditions may be a valid tool to understand giant pandas{\textquoteright} dietary habits, and provide valuable insights about what component of lignocellulose may be metabolized. Here, we collected gut microbiomes from fresh fecal samples of a giant panda (either entirely green or yellow stools) and supplied them with green leaves or yellow pith (i.e., the peeled stem). Microbial community composition was substrate dependent, and resulted in markedly different fermentation profiles, with yellow pith fermented to lactate and green leaves to lactate, acetate and ethanol, the latter to strikingly high concentrations (∼3%, v:v, within 3.5 h). Microbial metaproteins pointed to hemicellulose rather than cellulose degradation. The alpha-amylase from the giant panda (E.C. 3.2.1.1) was the predominant identified metaprotein, particularly in reactors inoculated with pellets derived from fecal samples (up to 60%). Gut microbiomes assemblage was most prominently impacted by the change in substrate (either leaf or pith). Removal of soluble organics from inocula to force lignocellulose degradation significantly enriched Bacteroides (in green leaf) and Escherichia/Shigella (in yellow pith). Overall, different substrates (either leaf or pith) markedly shaped gut microbiome assemblies and fermentation profiles. The biochemical profile of fermentation products may be an underestimated factor contributing to explain the peculiar dietary behavior of giant pandas, and should be implemented in large scale studies together with short-term lab-scale cultivation of gut microbiomes.",
keywords = "alpha amylase, cellulose, ethanol, fermentation, giant panda, gut microbiome, hemicellulose, lignocellulose",
author = "Alberto Scoma and Khor, {Way Cern} and Marta Coma and Robert Heyer and Ruben Props and Jonas Schoelynck and Tim Bouts and Dirk Benndorf and Desheng Li and Hemin Zhang and Korneel Rabaey",
year = "2020",
doi = "10.3389/fmicb.2020.00530",
language = "English",
volume = "11",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A",

}

RIS

TY - JOUR

T1 - Substrate-Dependent Fermentation of Bamboo in Giant Panda Gut Microbiomes

T2 - Leaf Primarily to Ethanol and Pith to Lactate

AU - Scoma, Alberto

AU - Khor, Way Cern

AU - Coma, Marta

AU - Heyer, Robert

AU - Props, Ruben

AU - Schoelynck, Jonas

AU - Bouts, Tim

AU - Benndorf, Dirk

AU - Li, Desheng

AU - Zhang, Hemin

AU - Rabaey, Korneel

PY - 2020

Y1 - 2020

N2 - The giant panda is known worldwide for having successfully moved to a diet almost exclusively based on bamboo. Provided that no lignocellulose-degrading enzyme was detected in panda’s genome, bamboo digestion is believed to depend on its gut microbiome. However, pandas retain the digestive system of a carnivore, with retention times of maximum 12 h. Cultivation of their unique gut microbiome under controlled laboratory conditions may be a valid tool to understand giant pandas’ dietary habits, and provide valuable insights about what component of lignocellulose may be metabolized. Here, we collected gut microbiomes from fresh fecal samples of a giant panda (either entirely green or yellow stools) and supplied them with green leaves or yellow pith (i.e., the peeled stem). Microbial community composition was substrate dependent, and resulted in markedly different fermentation profiles, with yellow pith fermented to lactate and green leaves to lactate, acetate and ethanol, the latter to strikingly high concentrations (∼3%, v:v, within 3.5 h). Microbial metaproteins pointed to hemicellulose rather than cellulose degradation. The alpha-amylase from the giant panda (E.C. 3.2.1.1) was the predominant identified metaprotein, particularly in reactors inoculated with pellets derived from fecal samples (up to 60%). Gut microbiomes assemblage was most prominently impacted by the change in substrate (either leaf or pith). Removal of soluble organics from inocula to force lignocellulose degradation significantly enriched Bacteroides (in green leaf) and Escherichia/Shigella (in yellow pith). Overall, different substrates (either leaf or pith) markedly shaped gut microbiome assemblies and fermentation profiles. The biochemical profile of fermentation products may be an underestimated factor contributing to explain the peculiar dietary behavior of giant pandas, and should be implemented in large scale studies together with short-term lab-scale cultivation of gut microbiomes.

AB - The giant panda is known worldwide for having successfully moved to a diet almost exclusively based on bamboo. Provided that no lignocellulose-degrading enzyme was detected in panda’s genome, bamboo digestion is believed to depend on its gut microbiome. However, pandas retain the digestive system of a carnivore, with retention times of maximum 12 h. Cultivation of their unique gut microbiome under controlled laboratory conditions may be a valid tool to understand giant pandas’ dietary habits, and provide valuable insights about what component of lignocellulose may be metabolized. Here, we collected gut microbiomes from fresh fecal samples of a giant panda (either entirely green or yellow stools) and supplied them with green leaves or yellow pith (i.e., the peeled stem). Microbial community composition was substrate dependent, and resulted in markedly different fermentation profiles, with yellow pith fermented to lactate and green leaves to lactate, acetate and ethanol, the latter to strikingly high concentrations (∼3%, v:v, within 3.5 h). Microbial metaproteins pointed to hemicellulose rather than cellulose degradation. The alpha-amylase from the giant panda (E.C. 3.2.1.1) was the predominant identified metaprotein, particularly in reactors inoculated with pellets derived from fecal samples (up to 60%). Gut microbiomes assemblage was most prominently impacted by the change in substrate (either leaf or pith). Removal of soluble organics from inocula to force lignocellulose degradation significantly enriched Bacteroides (in green leaf) and Escherichia/Shigella (in yellow pith). Overall, different substrates (either leaf or pith) markedly shaped gut microbiome assemblies and fermentation profiles. The biochemical profile of fermentation products may be an underestimated factor contributing to explain the peculiar dietary behavior of giant pandas, and should be implemented in large scale studies together with short-term lab-scale cultivation of gut microbiomes.

KW - alpha amylase

KW - cellulose

KW - ethanol

KW - fermentation

KW - giant panda

KW - gut microbiome

KW - hemicellulose

KW - lignocellulose

UR - http://www.scopus.com/inward/record.url?scp=85083346184&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2020.00530

DO - 10.3389/fmicb.2020.00530

M3 - Journal article

C2 - 32300339

AN - SCOPUS:85083346184

VL - 11

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 530

ER -