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Subretinal Saline Protects the Neuroretina From Thermic Damage During Laser Induction of Experimental Choroidal Neovascularization in Pigs

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Purpose: The purpose of this study was to develop a porcine model for photocoagulation
induced choroidal neovascularization (CNV) with high success rate and minimal
thermic damage to the neuroretina.
Methods: Experimental CNV was induced by laser photocoagulation in both eyes of 16
domestic pigs. In the left eyes, photocoagulation was preceded by subretinal injection
of saline to protect the neuroretina from thermic damage, whereas the right eyes were
treated with photocoagulation only. The development of the CNV after 3, 7, 14, 28, and
42 days was evaluated by optical coherence tomography (OCT) scanning, fluorescein
angiography, and OCT angiography, and by histology after enucleation.
Results: From day 7 after the photocoagulation, OCT showed subretinal density in all
lesions of 14 alive animals, and either fluorescein or OCT angiography confirmed CNV
formation in 11 of 14 of the eyes that had received photocoagulation alone and those
in which photocoagulation had been preceded by subretinal injection of saline. In all
cases pretreated with subretinal saline, the neuroretina was protected from immediate
thermic damage. The formation of CNVs were confirmed by histology. For both groups,
the largest lesions were observed within 14 days after photocoagulation.
Conclusions: Injection of subretinal saline can protect the retina fromthermic damage
induced by retinal photocoagulation without reducing the success rate in producing
experimental CNV. The effect of interventional studies aimed at reducing photocoagulation
induced experimental CNV in pigs can be evaluated within 2 weeks after photocoagulation.
Translational Relevance: This model provides a fundament to develop and evaluate
novel treatment methods for neovascular retinal diseases.
TidsskriftTranslational vision science & technology
StatusUdgivet - 29 jun. 2021

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