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Structural insights into the bacterial carbon - phosphorus lyase machinery

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

DOI

  • Paulina Seweryn, Danmark
  • Lan Bich Van
  • Morten Kjeldgaard, Danmark
  • Christoffer J. Russo, Medical Research Council Laboratory of Molecular Biology, Storbritannien
  • Lori A. Passmore, Medical Research Council Laboratory of Molecular Biology, Storbritannien
  • Bjarne Hove-Jensen, Danmark
  • Bjarne Jochimsen
    • ,
  • Ditlev E. Brodersen
Phosphorus is required for all life and microorganisms can extract it from their environment through several metabolic pathways. When phosphate is in limited supply, some bacteria are able to use phosphonate compounds, which require specialized enzymatic machinery to break the stable carbon–phosphorus (C–P) bond. Despite its importance, the details of how this machinery catabolizes phosphonates remain unknown. Here we determine the crystal structure of the 240-kilodalton Escherichia coli C–P lyase core complex (PhnG–PhnH–PhnI–PhnJ; PhnGHIJ), and show that it is a two-fold symmetric hetero-octamer comprising an intertwined network of subunits with unexpected self-homologies. It contains two potential active sites that probably couple phosphonate compounds to ATP and subsequently hydrolyse the C–P bond. We map the binding site of PhnK on the complex using electron microscopy, and show that it binds to a conserved insertion domain of PhnJ. Our results provide a structural basis for understanding microbial phosphonate breakdown.
OriginalsprogEngelsk
TidsskriftNature
Vol/bind525
Sider (fra-til)68-72
Antal sider5
ISSN0028-0836
DOI
StatusUdgivet - sep. 2015

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