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Structural insights into the architecture and assembly of eukaryotic flagella

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Cilia and flagella are slender projections found on most eukaryotic cells including unicellular organisms such as Chlamydomonas, Trypanosoma and Tetrahymena, where they serve motility and signaling functions. The cilium is a large molecular machine consisting of hundreds of different proteins that are trafficked into the organelle to organize a repetitive microtubule-based axoneme. Several recent studies took advantage of improved cryo-EM methodology to unravel the high-resolution structures of ciliary complexes. These include the recently reported purification and structure determination of axonemal doublet microtubules from the green algae Chlamydomonas reinhardtii, which allows for the modeling of more than 30 associated protein factors to provide deep molecular insight into the architecture and repetitive nature of doublet microtubules. In addition, we will review several recent contributions that dissect the structure and function of ciliary trafficking complexes that ferry structural and signaling components between the cell body and the cilium organelle.

TidsskriftMicrobial Cell
Sider (fra-til)289-299
Antal sider11
StatusUdgivet - nov. 2020

Bibliografisk note

Funding Information:
We thank the Novo Nordisk Foundation (grant no. NNF15OC0014164) and Carlsberg Foundation (grant no. CF19-0253) for funding this project. Narcis Adrian Petriman is supported by a postdoc fellowship from the European Commission (H2020, Grant Agreement number 888322). The figures were constructed with pymol [142].

Publisher Copyright:
© 2020 Petriman and Lorentzen.

Copyright 2020 Elsevier B.V., All rights reserved.

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