Structural insights into the architecture and assembly of eukaryotic flagella

TY - JOUR

T1 - Structural insights into the architecture and assembly of eukaryotic flagella

AU - Petriman, Narcis Adrian

AU - Lorentzen, Esben

N1 - Publisher Copyright: © 2020 Petriman and Lorentzen. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/11

Y1 - 2020/11

N2 - Cilia and flagella are slender projections found on most eukaryotic cells including unicellular organisms such as Chlamydomonas, Trypanosoma and Tetrahymena, where they serve motility and signaling functions. The cilium is a large molecular machine consisting of hundreds of different proteins that are trafficked into the organelle to organize a repetitive microtubule-based axoneme. Several recent studies took advantage of improved cryo-EM methodology to unravel the high-resolution structures of ciliary complexes. These include the recently reported purification and structure determination of axonemal doublet microtubules from the green algae Chlamydomonas reinhardtii, which allows for the modeling of more than 30 associated protein factors to provide deep molecular insight into the architecture and repetitive nature of doublet microtubules. In addition, we will review several recent contributions that dissect the structure and function of ciliary trafficking complexes that ferry structural and signaling components between the cell body and the cilium organelle.

AB - Cilia and flagella are slender projections found on most eukaryotic cells including unicellular organisms such as Chlamydomonas, Trypanosoma and Tetrahymena, where they serve motility and signaling functions. The cilium is a large molecular machine consisting of hundreds of different proteins that are trafficked into the organelle to organize a repetitive microtubule-based axoneme. Several recent studies took advantage of improved cryo-EM methodology to unravel the high-resolution structures of ciliary complexes. These include the recently reported purification and structure determination of axonemal doublet microtubules from the green algae Chlamydomonas reinhardtii, which allows for the modeling of more than 30 associated protein factors to provide deep molecular insight into the architecture and repetitive nature of doublet microtubules. In addition, we will review several recent contributions that dissect the structure and function of ciliary trafficking complexes that ferry structural and signaling components between the cell body and the cilium organelle.

KW - BBSome

KW - Cilia

KW - Intraflagellar transport (IFT)

KW - Microtubule doublets

UR - https://www.scopus.com/pages/publications/85098052234

U2 - 10.15698/MIC2020.11.734

DO - 10.15698/MIC2020.11.734

M3 - Review

AN - SCOPUS:85098052234

SN - 2311-2638

VL - 7

SP - 289

EP - 299

JO - Microbial Cell

JF - Microbial Cell

IS - 11

ER -