-
Uddannelse
Find uddannelse
Studievejledning
Kvalitet
- Forskning
-
Samarbejde
Virksomheder
Kommuner og regioner
Gymnasier
Myndigheder
-
Om AU
Organisation
Kontakt
Om AU
- Organisation
- Ledelse
- Strategi
- AU i tal
- AU's historie
- Internationalt samarbejde
- Bæredygtighed
- Campus 2.0
- Diversitet og ligestilling
- Ledige stillinger
- Presse
- Kontakt
Structural Basis for Dityrosine-Mediated Inhibition of α-Synuclein Fibrillization
Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review
Dokumenter
- jacs.2c03607
Forlagets udgivne version, 2,48 MB, PDF-dokument
DOI
- https://doi.org/10.1021/jacs.2c03607
Forlagets udgivne version
- Cagla Sahin ,
- Eva Christina Østerlund, Syddansk Universitet ,
- Nicklas Österlund, Stockholm University ,
- Joana Costeira-Paulo, Uppsala University ,
- Jannik Nedergaard Pedersen ,
- Gunna Christiansen, Aalborg Universitet ,
- Janni Nielsen
- Anne Louise Grønnemose, Syddansk Universitet ,
- Søren Kirk Amstrup ,
- Manish K. Tiwari, Københavns Universitet ,
- R. Shyama Prasad Rao, Yenepoya University ,
- Morten Jannik Bjerrum, Københavns Universitet ,
- Leopold L. Ilag, Stockholm University ,
- Michael J. Davies, Københavns Universitet ,
- Erik G. Marklund, Uppsala University ,
- Jan Skov Pedersen
- Michael Landreh, Karolinska Institutet ,
- Ian Max Møller
- Thomas J. D. Jørgensen, Syddansk Universitet ,
- Daniel Erik Otzen
α-Synuclein (α-Syn) is an intrinsically disordered protein which self-assembles into highly organized β-sheet structures that accumulate in plaques in brains of Parkinson's disease patients. Oxidative stress influences α-Syn structure and self-assembly; however, the basis for this remains unclear. Here we characterize the chemical and physical effects of mild oxidation on monomeric α-Syn and its aggregation. Using a combination of biophysical methods, small-angle X-ray scattering, and native ion mobility mass spectrometry, we find that oxidation leads to formation of intramolecular dityrosine cross-linkages and a compaction of the α-Syn monomer by a factor of √2. Oxidation-induced compaction is shown to inhibit ordered self-assembly and amyloid formation by steric hindrance, suggesting an important role of mild oxidation in preventing amyloid formation.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Journal of the American Chemical Society |
| Vol/bind | 144 |
| Nummer | 27 |
| Sider (fra-til) | 11949-11954 |
| Antal sider | 6 |
| ISSN | 0002-7863 |
| DOI | |
| Status | Udgivet - jul. 2022 |
Se relationer på Aarhus Universitet Citationsformater
Download-statistik
ID: 277685980