Strategies to identify potential therapeutic target sites in RNA

Publikation: Bidrag til bog/antologi/rapport/proceedingBidrag til bog/antologiForskningpeer review

Standard

Strategies to identify potential therapeutic target sites in RNA. / Lützelberger, Martin; Kjems, Jørgen.

Handbook of Experimental Pharmacology: RNA Towards Medicine. red. / Volker Erdmann; Jan Barciszewski; Jürgen Brosius. Bind 173 Berlin : Springer, 2006. s. 243-259.

Publikation: Bidrag til bog/antologi/rapport/proceedingBidrag til bog/antologiForskningpeer review

Harvard

Lützelberger, M & Kjems, J 2006, Strategies to identify potential therapeutic target sites in RNA. i V Erdmann, J Barciszewski & J Brosius (red), Handbook of Experimental Pharmacology: RNA Towards Medicine. bind 173, Springer, Berlin, s. 243-259. https://doi.org/10.1007/b138836

APA

Lützelberger, M., & Kjems, J. (2006). Strategies to identify potential therapeutic target sites in RNA. I V. Erdmann, J. Barciszewski, & J. Brosius (red.), Handbook of Experimental Pharmacology: RNA Towards Medicine (Bind 173, s. 243-259). Berlin: Springer. https://doi.org/10.1007/b138836

CBE

Lützelberger M, Kjems J. 2006. Strategies to identify potential therapeutic target sites in RNA. Erdmann V, Barciszewski J, Brosius J, red. I Handbook of Experimental Pharmacology: RNA Towards Medicine. Berlin: Springer. s. 243-259. https://doi.org/10.1007/b138836

MLA

Lützelberger, Martin og Jørgen Kjems "Strategies to identify potential therapeutic target sites in RNA"., Erdmann, Volker Barciszewski, Jan Brosius, Jürgen (red.). Handbook of Experimental Pharmacology: RNA Towards Medicine. Berlin: Springer. 2006, 243-259. https://doi.org/10.1007/b138836

Vancouver

Lützelberger M, Kjems J. Strategies to identify potential therapeutic target sites in RNA. I Erdmann V, Barciszewski J, Brosius J, red., Handbook of Experimental Pharmacology: RNA Towards Medicine. Bind 173. Berlin: Springer. 2006. s. 243-259 https://doi.org/10.1007/b138836

Author

Lützelberger, Martin ; Kjems, Jørgen. / Strategies to identify potential therapeutic target sites in RNA. Handbook of Experimental Pharmacology: RNA Towards Medicine. red. / Volker Erdmann ; Jan Barciszewski ; Jürgen Brosius. Bind 173 Berlin : Springer, 2006. s. 243-259

Bibtex

@inbook{0702168224b84779a0d5cf2ee7c03bbc,
title = "Strategies to identify potential therapeutic target sites in RNA",
abstract = "Antisense agents are powerful tools to inhibit gene expression in a sequence-specific manner. They are used for functional genomics, as diagnostic tools and for therapeutic purposes. Three classes of antisense agents can be distinguished by their mode of action: single-stranded antisense oligodeoxynucleotides; catalytic active RNA/DNA such as ribozymes, DNA- or locked nucleic acid (LNA)zymes; and small interfering RNA molecules known as siRNA. The selection of target sites in highly structured RNA molecules is crucial for their successful application. This is a difficult task, since RNA is assembled into nucleoprotein complexes and forms stable secondary structures in vivo, rendering most of the molecule inaccessible to intermolecular base pairing with complementary nucleic acids. In this review, we discuss several selection strategies to identify potential target sites in RNA molecules. In particular, we focus on combinatorial library approaches that allow high throughput screening of sequences for the design of antisense agents.",
keywords = "Animals, Gene Library, Humans, Oligonucleotides, Antisense, RNA, RNA, Catalytic, RNA, Small Interfering",
author = "Martin L{\"u}tzelberger and J{\o}rgen Kjems",
year = "2006",
doi = "10.1007/b138836",
language = "English",
isbn = "978-3-540-27261-8",
volume = "173",
pages = "243--259",
editor = "Volker Erdmann and Jan Barciszewski and J{\"u}rgen Brosius",
booktitle = "Handbook of Experimental Pharmacology",
publisher = "Springer",

}

RIS

TY - CHAP

T1 - Strategies to identify potential therapeutic target sites in RNA

AU - Lützelberger, Martin

AU - Kjems, Jørgen

PY - 2006

Y1 - 2006

N2 - Antisense agents are powerful tools to inhibit gene expression in a sequence-specific manner. They are used for functional genomics, as diagnostic tools and for therapeutic purposes. Three classes of antisense agents can be distinguished by their mode of action: single-stranded antisense oligodeoxynucleotides; catalytic active RNA/DNA such as ribozymes, DNA- or locked nucleic acid (LNA)zymes; and small interfering RNA molecules known as siRNA. The selection of target sites in highly structured RNA molecules is crucial for their successful application. This is a difficult task, since RNA is assembled into nucleoprotein complexes and forms stable secondary structures in vivo, rendering most of the molecule inaccessible to intermolecular base pairing with complementary nucleic acids. In this review, we discuss several selection strategies to identify potential target sites in RNA molecules. In particular, we focus on combinatorial library approaches that allow high throughput screening of sequences for the design of antisense agents.

AB - Antisense agents are powerful tools to inhibit gene expression in a sequence-specific manner. They are used for functional genomics, as diagnostic tools and for therapeutic purposes. Three classes of antisense agents can be distinguished by their mode of action: single-stranded antisense oligodeoxynucleotides; catalytic active RNA/DNA such as ribozymes, DNA- or locked nucleic acid (LNA)zymes; and small interfering RNA molecules known as siRNA. The selection of target sites in highly structured RNA molecules is crucial for their successful application. This is a difficult task, since RNA is assembled into nucleoprotein complexes and forms stable secondary structures in vivo, rendering most of the molecule inaccessible to intermolecular base pairing with complementary nucleic acids. In this review, we discuss several selection strategies to identify potential target sites in RNA molecules. In particular, we focus on combinatorial library approaches that allow high throughput screening of sequences for the design of antisense agents.

KW - Animals

KW - Gene Library

KW - Humans

KW - Oligonucleotides, Antisense

KW - RNA

KW - RNA, Catalytic

KW - RNA, Small Interfering

U2 - 10.1007/b138836

DO - 10.1007/b138836

M3 - Book chapter

C2 - 16594619

SN - 978-3-540-27261-8

VL - 173

SP - 243

EP - 259

BT - Handbook of Experimental Pharmacology

A2 - Erdmann, Volker

A2 - Barciszewski, Jan

A2 - Brosius, Jürgen

PB - Springer

CY - Berlin

ER -