Starch bioengineering affects cereal grain germination and seedling establishment

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Standard

Starch bioengineering affects cereal grain germination and seedling establishment. / Shaik, Shahnoor Sultana; Carciofi, Massimiliano; Martens, Helle Juel; Hebelstrup, Kim; Blennow, Andreas.

I: Journal of Experimental Botany, Bind 65, Nr. 9, 03.2014, s. 2257-2270.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Shaik, SS, Carciofi, M, Martens, HJ, Hebelstrup, K & Blennow, A 2014, 'Starch bioengineering affects cereal grain germination and seedling establishment' Journal of Experimental Botany, bind 65, nr. 9, s. 2257-2270. https://doi.org/10.1093/jxb/eru107

APA

Shaik, S. S., Carciofi, M., Martens, H. J., Hebelstrup, K., & Blennow, A. (2014). Starch bioengineering affects cereal grain germination and seedling establishment. Journal of Experimental Botany, 65(9), 2257-2270. https://doi.org/10.1093/jxb/eru107

CBE

Shaik SS, Carciofi M, Martens HJ, Hebelstrup K, Blennow A. 2014. Starch bioengineering affects cereal grain germination and seedling establishment. Journal of Experimental Botany. 65(9):2257-2270. https://doi.org/10.1093/jxb/eru107

MLA

Shaik, Shahnoor Sultana o.a.. "Starch bioengineering affects cereal grain germination and seedling establishment". Journal of Experimental Botany. 2014, 65(9). 2257-2270. https://doi.org/10.1093/jxb/eru107

Vancouver

Shaik SS, Carciofi M, Martens HJ, Hebelstrup K, Blennow A. Starch bioengineering affects cereal grain germination and seedling establishment. Journal of Experimental Botany. 2014 mar;65(9):2257-2270. https://doi.org/10.1093/jxb/eru107

Author

Shaik, Shahnoor Sultana ; Carciofi, Massimiliano ; Martens, Helle Juel ; Hebelstrup, Kim ; Blennow, Andreas. / Starch bioengineering affects cereal grain germination and seedling establishment. I: Journal of Experimental Botany. 2014 ; Bind 65, Nr. 9. s. 2257-2270.

Bibtex

@article{30966bf8e9a44daabcab555436af1036,
title = "Starch bioengineering affects cereal grain germination and seedling establishment",
abstract = "Cereal grain germination is central for plant early development, and efficient germination has a major role in crop propagation and malting. Endosperm starch is the prime energy reserve in germination and seedling establishment. In this study, it was hypothesized that optimized starch granule structure, and not only the endosperm starch content per se, is important for germination and seedling establishment. For that purpose, wild-type (WT), and specifically engineered degradable hyperphosphorylated (HP) starch and more resistant amylose-only (AO) starch barley lines were used. The transgenics showed no severe phenotypes and the WT and HP lines degraded the starch similarly, having 30{\%} residual starch after 12 d of germination. However, the AO line showed significant resistance to degradation, having 57{\%} residual starch. Interestingly, protein and β-glucan (BG) degradation was stimulated for both HP and AO lines as compared with the WT. At late seedling establishment stages, specific sugars were rapidly consumed in the AO line. α-Amylase activity was distinctly suppressed in both the HP and the AO lines. Pre-germination β-amylase deposition was low in the AO grains and β-amylase was generally suppressed in both HP and AO lines throughout germination. As further supported by scanning electron microscopy and histochemical analyses on grain and seedlings, it was concluded that inadequate starch granule deposition in combination with the suppressed hydrolase activity leads to temporal and compensating re-direction of starch, sugar, and protein catabolism important to maintain metabolic dynamics during grain germination and seedling establishment.",
keywords = "Amylase, barley, cereal, germination, grain, starch",
author = "Shaik, {Shahnoor Sultana} and Massimiliano Carciofi and Martens, {Helle Juel} and Kim Hebelstrup and Andreas Blennow",
year = "2014",
month = "3",
doi = "10.1093/jxb/eru107",
language = "English",
volume = "65",
pages = "2257--2270",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "9",

}

RIS

TY - JOUR

T1 - Starch bioengineering affects cereal grain germination and seedling establishment

AU - Shaik, Shahnoor Sultana

AU - Carciofi, Massimiliano

AU - Martens, Helle Juel

AU - Hebelstrup, Kim

AU - Blennow, Andreas

PY - 2014/3

Y1 - 2014/3

N2 - Cereal grain germination is central for plant early development, and efficient germination has a major role in crop propagation and malting. Endosperm starch is the prime energy reserve in germination and seedling establishment. In this study, it was hypothesized that optimized starch granule structure, and not only the endosperm starch content per se, is important for germination and seedling establishment. For that purpose, wild-type (WT), and specifically engineered degradable hyperphosphorylated (HP) starch and more resistant amylose-only (AO) starch barley lines were used. The transgenics showed no severe phenotypes and the WT and HP lines degraded the starch similarly, having 30% residual starch after 12 d of germination. However, the AO line showed significant resistance to degradation, having 57% residual starch. Interestingly, protein and β-glucan (BG) degradation was stimulated for both HP and AO lines as compared with the WT. At late seedling establishment stages, specific sugars were rapidly consumed in the AO line. α-Amylase activity was distinctly suppressed in both the HP and the AO lines. Pre-germination β-amylase deposition was low in the AO grains and β-amylase was generally suppressed in both HP and AO lines throughout germination. As further supported by scanning electron microscopy and histochemical analyses on grain and seedlings, it was concluded that inadequate starch granule deposition in combination with the suppressed hydrolase activity leads to temporal and compensating re-direction of starch, sugar, and protein catabolism important to maintain metabolic dynamics during grain germination and seedling establishment.

AB - Cereal grain germination is central for plant early development, and efficient germination has a major role in crop propagation and malting. Endosperm starch is the prime energy reserve in germination and seedling establishment. In this study, it was hypothesized that optimized starch granule structure, and not only the endosperm starch content per se, is important for germination and seedling establishment. For that purpose, wild-type (WT), and specifically engineered degradable hyperphosphorylated (HP) starch and more resistant amylose-only (AO) starch barley lines were used. The transgenics showed no severe phenotypes and the WT and HP lines degraded the starch similarly, having 30% residual starch after 12 d of germination. However, the AO line showed significant resistance to degradation, having 57% residual starch. Interestingly, protein and β-glucan (BG) degradation was stimulated for both HP and AO lines as compared with the WT. At late seedling establishment stages, specific sugars were rapidly consumed in the AO line. α-Amylase activity was distinctly suppressed in both the HP and the AO lines. Pre-germination β-amylase deposition was low in the AO grains and β-amylase was generally suppressed in both HP and AO lines throughout germination. As further supported by scanning electron microscopy and histochemical analyses on grain and seedlings, it was concluded that inadequate starch granule deposition in combination with the suppressed hydrolase activity leads to temporal and compensating re-direction of starch, sugar, and protein catabolism important to maintain metabolic dynamics during grain germination and seedling establishment.

KW - Amylase

KW - barley

KW - cereal

KW - germination

KW - grain

KW - starch

U2 - 10.1093/jxb/eru107

DO - 10.1093/jxb/eru107

M3 - Journal article

VL - 65

SP - 2257

EP - 2270

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 9

ER -