Standardized Protocol for the Preparation of Precision-Cut Kidney Slices:: A Translational Model of Renal Fibrosis

Michael Schou Jensen; Camilla Merrild; Rikke Nørregaard; Peter Olinga; Henricus A M Mutsaers

doi:10.1007/978-1-0716-3179-9_9

Standardized Protocol for the Preparation of Precision-Cut Kidney Slices: A Translational Model of Renal Fibrosis

Michael Schou Jensen, Camilla Merrild, Rikke Nørregaard, Peter Olinga, Henricus A M Mutsaers

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

1 Citationer (Scopus)

Abstract

Renal fibrosis is a hallmark of progressive renal diseases. To date, there is a lack of effective therapeutics for the treatment of renal fibrosis, in part due to the scarcity of clinically relevant translational disease models. Since the early 1920s, hand-cut tissue slices have been used as a means to better understand organ (patho)physiology in a variety of scientific fields. From that time, the equipment and methodology for the preparation of tissue slices has continuously improved, thereby expanding the applicability of the model. Nowadays, precision-cut kidney slices (PCKS) have been demonstrated to be an extremely valuable translation model for renal (patho)physiology, bridging the gap between preclinical and clinical research. A key feature of PCKS is that the slices contain all cell types and acellular components of the whole organ in the original configuration while preserving cell-cell and cell-matrix interactions. In this chapter, we describe how to prepare PCKS and how the model can be implemented in fibrosis research.

Originalsprog	Engelsk
Tidsskrift	Methods in molecular biology (Clifton, N.J.)
Vol/bind	2664
Sider (fra-til)	123-134
Antal sider	12
ISSN	1064-3745
DOI	https://doi.org/10.1007/978-1-0716-3179-9_9
Status	Udgivet - 2023

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Citationsformater

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abstract = "Renal fibrosis is a hallmark of progressive renal diseases. To date, there is a lack of effective therapeutics for the treatment of renal fibrosis, in part due to the scarcity of clinically relevant translational disease models. Since the early 1920s, hand-cut tissue slices have been used as a means to better understand organ (patho)physiology in a variety of scientific fields. From that time, the equipment and methodology for the preparation of tissue slices has continuously improved, thereby expanding the applicability of the model. Nowadays, precision-cut kidney slices (PCKS) have been demonstrated to be an extremely valuable translation model for renal (patho)physiology, bridging the gap between preclinical and clinical research. A key feature of PCKS is that the slices contain all cell types and acellular components of the whole organ in the original configuration while preserving cell-cell and cell-matrix interactions. In this chapter, we describe how to prepare PCKS and how the model can be implemented in fibrosis research.",

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Standardized Protocol for the Preparation of Precision-Cut Kidney Slices: A Translational Model of Renal Fibrosis. / Jensen, Michael Schou ; Merrild, Camilla ; Nørregaard, Rikke et al.
I: Methods in molecular biology (Clifton, N.J.), Bind 2664, 2023, s. 123-134.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

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T2 - A Translational Model of Renal Fibrosis

AU - Jensen, Michael Schou

AU - Merrild, Camilla

AU - Nørregaard, Rikke

AU - Olinga, Peter

AU - Mutsaers, Henricus A M

PY - 2023

Y1 - 2023

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AB - Renal fibrosis is a hallmark of progressive renal diseases. To date, there is a lack of effective therapeutics for the treatment of renal fibrosis, in part due to the scarcity of clinically relevant translational disease models. Since the early 1920s, hand-cut tissue slices have been used as a means to better understand organ (patho)physiology in a variety of scientific fields. From that time, the equipment and methodology for the preparation of tissue slices has continuously improved, thereby expanding the applicability of the model. Nowadays, precision-cut kidney slices (PCKS) have been demonstrated to be an extremely valuable translation model for renal (patho)physiology, bridging the gap between preclinical and clinical research. A key feature of PCKS is that the slices contain all cell types and acellular components of the whole organ in the original configuration while preserving cell-cell and cell-matrix interactions. In this chapter, we describe how to prepare PCKS and how the model can be implemented in fibrosis research.

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KW - Kidney Diseases/metabolism

KW - Kidney/metabolism

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