Staining protocols to improve the detection of composite restorations in human identification

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Staining protocols to improve the detection of composite restorations in human identification. / Conceição, Luciana D.; de Pereira, Claudio M.P.; Forgie, Andrew H.; Leite, Fábio R.M.

I: Forensic Science International, Bind 297, 01.04.2019, s. 198-203.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Conceição, LD, de Pereira, CMP, Forgie, AH & Leite, FRM 2019, 'Staining protocols to improve the detection of composite restorations in human identification', Forensic Science International, bind 297, s. 198-203. https://doi.org/10.1016/j.forsciint.2019.01.049

APA

Conceição, L. D., de Pereira, C. M. P., Forgie, A. H., & Leite, F. R. M. (2019). Staining protocols to improve the detection of composite restorations in human identification. Forensic Science International, 297, 198-203. https://doi.org/10.1016/j.forsciint.2019.01.049

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MLA

Vancouver

Conceição LD, de Pereira CMP, Forgie AH, Leite FRM. Staining protocols to improve the detection of composite restorations in human identification. Forensic Science International. 2019 apr 1;297:198-203. https://doi.org/10.1016/j.forsciint.2019.01.049

Author

Conceição, Luciana D. ; de Pereira, Claudio M.P. ; Forgie, Andrew H. ; Leite, Fábio R.M. / Staining protocols to improve the detection of composite restorations in human identification. I: Forensic Science International. 2019 ; Bind 297. s. 198-203.

Bibtex

@article{5c50759a594746ceb01060dec9ebe2e9,
title = "Staining protocols to improve the detection of composite restorations in human identification",
abstract = "Introduction: The improvement of aesthetic properties of dental materials has hampered the work of forensic experts in cases of identification. Even in dental practice, the identification of the margins of restorations has become a challenge. Objective: To establish protocols to improve the visual contrast between teeth and composites using dyes. Method: Anterior and premolar human teeth were chosen (n = 40) and class V cavities were made in the lingual/palatal and buccal surfaces. Ten commercially available dyes were dissolved in distilled water. Three protocols were proposed using phosphoric acid (Gphos) and hydrofluoric acid (Ghydro) for 60 s followed by application of the dye for 20 s. The control group (Gcontrol) was acid free, i.e. only distilled water was applied. Data was analysed using Kruskal-Wallis and Dunn´s tests. Results: Analyses showed that darker dyes, such as crystal violet, methylene blue, malachite green and neutral red, had better results (p < 0.001). The composite brand did not influence the results (p > 0.05). Both Gphos and Ghydro were effective in discriminating restorations when compared to Gcontrol (p > 0.001). No differences were detected between Gphos and Ghydro protocols (p > 0.05). In Gphos, the enamel surface was stained leaving the restoration without pigmentation. Oppositely, in Ghydro the composite filling was coloured, but not the enamel. In Gcontrol, both enamel and restoration were stained indistinctively. Conclusion: Tooth etching with either phosphoric or hydrofluoric acids was suitable to discriminate the presence of aesthetic dental fillings.",
keywords = "Dental materials, Dyes, Forensic dentistry, Human identification, Staining",
author = "Concei{\c c}{\~a}o, {Luciana D.} and {de Pereira}, {Claudio M.P.} and Forgie, {Andrew H.} and Leite, {F{\'a}bio R.M.}",
year = "2019",
month = apr,
day = "1",
doi = "10.1016/j.forsciint.2019.01.049",
language = "English",
volume = "297",
pages = "198--203",
journal = "Forensic Science International",
issn = "0379-0738",
publisher = "Elsevier Ireland Ltd.",

}

RIS

TY - JOUR

T1 - Staining protocols to improve the detection of composite restorations in human identification

AU - Conceição, Luciana D.

AU - de Pereira, Claudio M.P.

AU - Forgie, Andrew H.

AU - Leite, Fábio R.M.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Introduction: The improvement of aesthetic properties of dental materials has hampered the work of forensic experts in cases of identification. Even in dental practice, the identification of the margins of restorations has become a challenge. Objective: To establish protocols to improve the visual contrast between teeth and composites using dyes. Method: Anterior and premolar human teeth were chosen (n = 40) and class V cavities were made in the lingual/palatal and buccal surfaces. Ten commercially available dyes were dissolved in distilled water. Three protocols were proposed using phosphoric acid (Gphos) and hydrofluoric acid (Ghydro) for 60 s followed by application of the dye for 20 s. The control group (Gcontrol) was acid free, i.e. only distilled water was applied. Data was analysed using Kruskal-Wallis and Dunn´s tests. Results: Analyses showed that darker dyes, such as crystal violet, methylene blue, malachite green and neutral red, had better results (p < 0.001). The composite brand did not influence the results (p > 0.05). Both Gphos and Ghydro were effective in discriminating restorations when compared to Gcontrol (p > 0.001). No differences were detected between Gphos and Ghydro protocols (p > 0.05). In Gphos, the enamel surface was stained leaving the restoration without pigmentation. Oppositely, in Ghydro the composite filling was coloured, but not the enamel. In Gcontrol, both enamel and restoration were stained indistinctively. Conclusion: Tooth etching with either phosphoric or hydrofluoric acids was suitable to discriminate the presence of aesthetic dental fillings.

AB - Introduction: The improvement of aesthetic properties of dental materials has hampered the work of forensic experts in cases of identification. Even in dental practice, the identification of the margins of restorations has become a challenge. Objective: To establish protocols to improve the visual contrast between teeth and composites using dyes. Method: Anterior and premolar human teeth were chosen (n = 40) and class V cavities were made in the lingual/palatal and buccal surfaces. Ten commercially available dyes were dissolved in distilled water. Three protocols were proposed using phosphoric acid (Gphos) and hydrofluoric acid (Ghydro) for 60 s followed by application of the dye for 20 s. The control group (Gcontrol) was acid free, i.e. only distilled water was applied. Data was analysed using Kruskal-Wallis and Dunn´s tests. Results: Analyses showed that darker dyes, such as crystal violet, methylene blue, malachite green and neutral red, had better results (p < 0.001). The composite brand did not influence the results (p > 0.05). Both Gphos and Ghydro were effective in discriminating restorations when compared to Gcontrol (p > 0.001). No differences were detected between Gphos and Ghydro protocols (p > 0.05). In Gphos, the enamel surface was stained leaving the restoration without pigmentation. Oppositely, in Ghydro the composite filling was coloured, but not the enamel. In Gcontrol, both enamel and restoration were stained indistinctively. Conclusion: Tooth etching with either phosphoric or hydrofluoric acids was suitable to discriminate the presence of aesthetic dental fillings.

KW - Dental materials

KW - Dyes

KW - Forensic dentistry

KW - Human identification

KW - Staining

UR - http://www.scopus.com/inward/record.url?scp=85062149099&partnerID=8YFLogxK

U2 - 10.1016/j.forsciint.2019.01.049

DO - 10.1016/j.forsciint.2019.01.049

M3 - Journal article

C2 - 30826603

AN - SCOPUS:85062149099

VL - 297

SP - 198

EP - 203

JO - Forensic Science International

JF - Forensic Science International

SN - 0379-0738

ER -