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Specific detection of topoisomerase i from the malaria causing P. falciparum parasite using isothermal Rolling Circle Amplification

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We present a Rolling-Circle-Enhance-Enzyme-Activity-Detection (REEAD) system with potential use for future point-of-care diagnosis of malaria. In the developed setup, specific detection of malaria parasites in crude blood samples is facilitated by the conversion of single Plasmodium falciparum topoisomerase I (pfTopI) mediated cleavage-ligation events, happening within nanometer dimensions, to micrometer-sized products readily detectable at the single molecule level in a fluorescence microscope. In principle, REEAD requires no special equipment and the readout is adaptable to simple colorimetric detection systems. Moreover, with regard to detection limit the presented setup is likely to outcompete standard gold immuno-based diagnostics. Hence, we believe the presented assay forms the basis for a new generation of easy-to-use diagnostic tools suitable for the malaria epidemic areas in developing countries.
OriginalsprogEngelsk
TidsskriftI E E E Engineering in Medicine and Biology Society. Conference Proceedings
Vol/bind2012
Sider (fra-til)2416-2419
Antal sider4
ISSN2375-7477
DOI
StatusUdgivet - aug. 2012
Begivenhed34th Annual International Conference of IEEE - San Diego, USA
Varighed: 28 aug. 20121 sep. 2012
Konferencens nummer: 34th

Konference

Konference34th Annual International Conference of IEEE
Nummer34th
LandUSA
BySan Diego
Periode28/08/201201/09/2012

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