Specific detection of topoisomerase i from the malaria causing P. falciparum parasite using isothermal Rolling Circle Amplification

TY - GEN

T1 - Specific detection of topoisomerase i from the malaria causing P. falciparum parasite using isothermal Rolling Circle Amplification

AU - Tesauro, Cinzia

AU - Juul, Sissel

AU - Arno, Barbara

AU - Nielsen, Christine J F

AU - Fiorani, Paola

AU - Frøhlich, Rikke F

AU - Andersen, Felicie F

AU - Desideri, Alessandro

AU - Stougaard, Magnus

AU - Petersen, Eskild

AU - Knudsen, Birgitta R

N1 - Conference code: 34th

PY - 2012/8

Y1 - 2012/8

N2 - We present a Rolling-Circle-Enhance-Enzyme-Activity-Detection (REEAD) system with potential use for future point-of-care diagnosis of malaria. In the developed setup, specific detection of malaria parasites in crude blood samples is facilitated by the conversion of single Plasmodium falciparum topoisomerase I (pfTopI) mediated cleavage-ligation events, happening within nanometer dimensions, to micrometer-sized products readily detectable at the single molecule level in a fluorescence microscope. In principle, REEAD requires no special equipment and the readout is adaptable to simple colorimetric detection systems. Moreover, with regard to detection limit the presented setup is likely to outcompete standard gold immuno-based diagnostics. Hence, we believe the presented assay forms the basis for a new generation of easy-to-use diagnostic tools suitable for the malaria epidemic areas in developing countries.

AB - We present a Rolling-Circle-Enhance-Enzyme-Activity-Detection (REEAD) system with potential use for future point-of-care diagnosis of malaria. In the developed setup, specific detection of malaria parasites in crude blood samples is facilitated by the conversion of single Plasmodium falciparum topoisomerase I (pfTopI) mediated cleavage-ligation events, happening within nanometer dimensions, to micrometer-sized products readily detectable at the single molecule level in a fluorescence microscope. In principle, REEAD requires no special equipment and the readout is adaptable to simple colorimetric detection systems. Moreover, with regard to detection limit the presented setup is likely to outcompete standard gold immuno-based diagnostics. Hence, we believe the presented assay forms the basis for a new generation of easy-to-use diagnostic tools suitable for the malaria epidemic areas in developing countries.

U2 - 10.1109/EMBC.2012.6346451

DO - 10.1109/EMBC.2012.6346451

M3 - Conference article

C2 - 23366412

SN - 2375-7477

VL - 2012

SP - 2416

EP - 2419

JO - I E E E Engineering in Medicine and Biology Society. Conference Proceedings

JF - I E E E Engineering in Medicine and Biology Society. Conference Proceedings

T2 - 34th Annual International Conference of IEEE

Y2 - 28 August 2012 through 1 September 2012

ER -