Aarhus University Seal / Aarhus Universitets segl

Specific detection of the cleavage activity of mycobacterial enzymes using a quantum dot based DNA nanosensor

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

DOI

  • Morten Leth Jepsen
  • ,
  • Charlotte Harmsen
  • ,
  • Adwait Anand Godbole, Danmark
  • Valakunja Nagaraja, Danmark
  • Birgitta R Knudsen
  • Yi-Ping Ho

We present a quantum dot based DNA nanosensor specifically targeting the cleavage step in the reaction cycle of the essential DNA-modifying enzyme, mycobacterial topoisomerase I. The design takes advantages of the unique photophysical properties of quantum dots to generate visible fluorescence recovery upon specific cleavage by mycobacterial topoisomerase I. This report, for the first time, demonstrates the possibility to quantify the cleavage activity of the mycobacterial enzyme without the pre-processing sample purification or post-processing signal amplification. The cleavage induced signal response has also proven reliable in biological matrices, such as whole cell extracts prepared from Escherichia coli and human Caco-2 cells. It is expected that the assay may contribute to the clinical diagnostics of bacterial diseases, as well as the evaluation of treatment outcomes.

OriginalsprogEngelsk
TidsskriftNanoscale
Vol/bind8
Nummer1
Sider (fra-til)358-364
Antal sider7
ISSN2040-3364
DOI
StatusUdgivet - 2016

Se relationer på Aarhus Universitet Citationsformater

ID: 95364338