Specific detection of the cleavage activity of mycobacterial enzymes using a quantum dot based DNA nanosensor

Morten Leth Jepsen; Charlotte Harmsen; Adwait Anand Godbole; Valakunja Nagaraja; Birgitta R Knudsen; Yi-Ping Ho

doi:10.1039/c5nr06326d

Specific detection of the cleavage activity of mycobacterial enzymes using a quantum dot based DNA nanosensor

Morten Leth Jepsen, Charlotte Harmsen, Adwait Anand Godbole, Valakunja Nagaraja, Birgitta R Knudsen, Yi-Ping Ho

Interdisciplinary Nanoscience Center - iNANO-MBG, Universitetsbyen

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

13 Citationer (Scopus)

Abstract

We present a quantum dot based DNA nanosensor specifically targeting the cleavage step in the reaction cycle of the essential DNA-modifying enzyme, mycobacterial topoisomerase I. The design takes advantages of the unique photophysical properties of quantum dots to generate visible fluorescence recovery upon specific cleavage by mycobacterial topoisomerase I. This report, for the first time, demonstrates the possibility to quantify the cleavage activity of the mycobacterial enzyme without the pre-processing sample purification or post-processing signal amplification. The cleavage induced signal response has also proven reliable in biological matrices, such as whole cell extracts prepared from Escherichia coli and human Caco-2 cells. It is expected that the assay may contribute to the clinical diagnostics of bacterial diseases, as well as the evaluation of treatment outcomes.

Originalsprog	Engelsk
Tidsskrift	Nanoscale
Vol/bind	8
Nummer	1
Sider (fra-til)	358-364
Antal sider	7
ISSN	2040-3364
DOI	https://doi.org/10.1039/c5nr06326d
Status	Udgivet - 2016

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10.1039/c5nr06326d

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abstract = "We present a quantum dot based DNA nanosensor specifically targeting the cleavage step in the reaction cycle of the essential DNA-modifying enzyme, mycobacterial topoisomerase I. The design takes advantages of the unique photophysical properties of quantum dots to generate visible fluorescence recovery upon specific cleavage by mycobacterial topoisomerase I. This report, for the first time, demonstrates the possibility to quantify the cleavage activity of the mycobacterial enzyme without the pre-processing sample purification or post-processing signal amplification. The cleavage induced signal response has also proven reliable in biological matrices, such as whole cell extracts prepared from Escherichia coli and human Caco-2 cells. It is expected that the assay may contribute to the clinical diagnostics of bacterial diseases, as well as the evaluation of treatment outcomes.",

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T1 - Specific detection of the cleavage activity of mycobacterial enzymes using a quantum dot based DNA nanosensor

AU - Jepsen, Morten Leth

AU - Harmsen, Charlotte

AU - Godbole, Adwait Anand

AU - Nagaraja, Valakunja

AU - Knudsen, Birgitta R

AU - Ho, Yi-Ping

PY - 2016

Y1 - 2016

N2 - We present a quantum dot based DNA nanosensor specifically targeting the cleavage step in the reaction cycle of the essential DNA-modifying enzyme, mycobacterial topoisomerase I. The design takes advantages of the unique photophysical properties of quantum dots to generate visible fluorescence recovery upon specific cleavage by mycobacterial topoisomerase I. This report, for the first time, demonstrates the possibility to quantify the cleavage activity of the mycobacterial enzyme without the pre-processing sample purification or post-processing signal amplification. The cleavage induced signal response has also proven reliable in biological matrices, such as whole cell extracts prepared from Escherichia coli and human Caco-2 cells. It is expected that the assay may contribute to the clinical diagnostics of bacterial diseases, as well as the evaluation of treatment outcomes.

AB - We present a quantum dot based DNA nanosensor specifically targeting the cleavage step in the reaction cycle of the essential DNA-modifying enzyme, mycobacterial topoisomerase I. The design takes advantages of the unique photophysical properties of quantum dots to generate visible fluorescence recovery upon specific cleavage by mycobacterial topoisomerase I. This report, for the first time, demonstrates the possibility to quantify the cleavage activity of the mycobacterial enzyme without the pre-processing sample purification or post-processing signal amplification. The cleavage induced signal response has also proven reliable in biological matrices, such as whole cell extracts prepared from Escherichia coli and human Caco-2 cells. It is expected that the assay may contribute to the clinical diagnostics of bacterial diseases, as well as the evaluation of treatment outcomes.

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DO - 10.1039/c5nr06326d

M3 - Journal article

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SN - 2040-3364

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SP - 358

EP - 364

JO - Nanoscale

JF - Nanoscale

IS - 1

ER -

Specific detection of the cleavage activity of mycobacterial enzymes using a quantum dot based DNA nanosensor

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