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Spatially Controlled Delivery of siRNAs to Stem Cells in Implants Generated by Multi-Component Additive Manufacturing

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Spatially Controlled Delivery of siRNAs to Stem Cells in Implants Generated by Multi-Component Additive Manufacturing. / Andersen, Morten Østergaard; Le, Dang Quang Svend; Chen, Muwan; Nygaard, Jens Vinge; Kassem, Moustapha; Bünger, Cody; Kjems, Jørgen.

I: Advanced Functional Materials, Bind 23, Nr. 45, 05.12.2013, s. 5599-5607.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{1412021329db431eb1c330f9bd3e4eeb,
title = "Spatially Controlled Delivery of siRNAs to Stem Cells in Implants Generated by Multi-Component Additive Manufacturing",
abstract = "Additive manufacturing is a promising technique in tissue engineering, as it enables truly individualized implants to be made to fit a particular defect. As previously shown, a feasible strategy to produce complex multicellular tissues is to deposit different small interfering RNA (siRNA) in porous implants that are subsequently sutured together. In this study, an additive manufacturing strategy to deposit carbohydrate hydrogels containing different siRNAs is applied into an implant, in a spatially controlled manner. When the obtained structures are seeded with mesenchymal stem (stromal) cells, the selected siRNAs are delivered to the cells and induces specific and localized gene silencing. Here, it is demonstrated how to replicate part of a patient's spinal cord from a computed tomography scan, using an additive manufacturing technique to produce an implant with compartmentalized siRNAs in the locations corresponding to distinct tissue. Hydrogel solutions loaded with different siRNA can be co-printed together with polycaprolactone that acts as rigid mechanical support to the hydrogel. This study demonstrates a new route for the production of 3D functionalized, individualized implants which may provide great clinical benefit",
keywords = "drug delivery, hydrogels, medical applications, polymeric materials, tissue engineering",
author = "Andersen, {Morten {\O}stergaard} and Le, {Dang Quang Svend} and Muwan Chen and Nygaard, {Jens Vinge} and Moustapha Kassem and Cody B{\"u}nger and J{\o}rgen Kjems",
year = "2013",
month = dec,
day = "5",
doi = "10.1002/adfm.201300832",
language = "English",
volume = "23",
pages = "5599--5607",
journal = "Advanced Functional Materials",
issn = "1616-301X",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "45",

}

RIS

TY - JOUR

T1 - Spatially Controlled Delivery of siRNAs to Stem Cells in Implants Generated by Multi-Component Additive Manufacturing

AU - Andersen, Morten Østergaard

AU - Le, Dang Quang Svend

AU - Chen, Muwan

AU - Nygaard, Jens Vinge

AU - Kassem, Moustapha

AU - Bünger, Cody

AU - Kjems, Jørgen

PY - 2013/12/5

Y1 - 2013/12/5

N2 - Additive manufacturing is a promising technique in tissue engineering, as it enables truly individualized implants to be made to fit a particular defect. As previously shown, a feasible strategy to produce complex multicellular tissues is to deposit different small interfering RNA (siRNA) in porous implants that are subsequently sutured together. In this study, an additive manufacturing strategy to deposit carbohydrate hydrogels containing different siRNAs is applied into an implant, in a spatially controlled manner. When the obtained structures are seeded with mesenchymal stem (stromal) cells, the selected siRNAs are delivered to the cells and induces specific and localized gene silencing. Here, it is demonstrated how to replicate part of a patient's spinal cord from a computed tomography scan, using an additive manufacturing technique to produce an implant with compartmentalized siRNAs in the locations corresponding to distinct tissue. Hydrogel solutions loaded with different siRNA can be co-printed together with polycaprolactone that acts as rigid mechanical support to the hydrogel. This study demonstrates a new route for the production of 3D functionalized, individualized implants which may provide great clinical benefit

AB - Additive manufacturing is a promising technique in tissue engineering, as it enables truly individualized implants to be made to fit a particular defect. As previously shown, a feasible strategy to produce complex multicellular tissues is to deposit different small interfering RNA (siRNA) in porous implants that are subsequently sutured together. In this study, an additive manufacturing strategy to deposit carbohydrate hydrogels containing different siRNAs is applied into an implant, in a spatially controlled manner. When the obtained structures are seeded with mesenchymal stem (stromal) cells, the selected siRNAs are delivered to the cells and induces specific and localized gene silencing. Here, it is demonstrated how to replicate part of a patient's spinal cord from a computed tomography scan, using an additive manufacturing technique to produce an implant with compartmentalized siRNAs in the locations corresponding to distinct tissue. Hydrogel solutions loaded with different siRNA can be co-printed together with polycaprolactone that acts as rigid mechanical support to the hydrogel. This study demonstrates a new route for the production of 3D functionalized, individualized implants which may provide great clinical benefit

KW - drug delivery

KW - hydrogels

KW - medical applications

KW - polymeric materials

KW - tissue engineering

U2 - 10.1002/adfm.201300832

DO - 10.1002/adfm.201300832

M3 - Journal article

AN - SCOPUS:84878348964

VL - 23

SP - 5599

EP - 5607

JO - Advanced Functional Materials

JF - Advanced Functional Materials

SN - 1616-301X

IS - 45

ER -