Spatial and temporal expression patterns of chitinase genes in developing zebrafish embryos

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Spatial and temporal expression patterns of chitinase genes in developing zebrafish embryos. / Koch, Bjørn Erik Vind; Stougaard, Jens; Spaink, Herman P.

I: Gene Expression Patterns, Bind 14, Nr. 2, 03.2014, s. 69-77.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

APA

CBE

MLA

Vancouver

Author

Koch, Bjørn Erik Vind ; Stougaard, Jens ; Spaink, Herman P. / Spatial and temporal expression patterns of chitinase genes in developing zebrafish embryos. I: Gene Expression Patterns. 2014 ; Bind 14, Nr. 2. s. 69-77.

Bibtex

@article{d67cf54bc4564911b4c97cb58008e7fc,
title = "Spatial and temporal expression patterns of chitinase genes in developing zebrafish embryos",
abstract = "Chitinases and chitinase like proteins play an important role in mammalian immunity and functions in early zebrafish development have been suggested. Here we report identification of six zebrafish chitinases and chitinase like proteins (called CHIA.1-6) belonging to the glycoside hydrolase family 18, and determine their spatial and temporal expression at 10 stages of zebrafish development. CHIA.4 is highly maternally expressed and it is expressed 100 fold above any other CHIA gene at zygote through to blastula stage. Later, after the maternal to zygotic transition, CHIA.4 expression decreases to the same level as CHIA.5 and CHIA.6. Subsequently, CHIA.1, CHIA.2, CHIA.3 and CHIA.4, CHIA.5, CHIA.6 each follow distinct paths in terms of expression levels. Until 4days post fertilization the spatial expression patterns of all six CHIA genes overlap extensively, with expression detected predominantly in vascular, ocular and intestinal tissues. At 5days post fertilization CHIA.1, CHIA.2 and CHIA.3 are expressed almost exclusively in the stomach, whereas CHIA.4, CHIA.5 and CHIA.6 are also prominently expressed in the liver. These different expression patterns may contribute to the establishment of a basis, on which functional analysis in older larvae may be founded.",
author = "Koch, {Bj{\o}rn Erik Vind} and Jens Stougaard and Spaink, {Herman P}",
note = "Copyright {\textcopyright} 2014. Published by Elsevier B.V.",
year = "2014",
month = mar,
doi = "10.1016/j.gep.2014.01.001",
language = "English",
volume = "14",
pages = "69--77",
journal = "Gene Expression Patterns",
issn = "1567-133X",
publisher = "Elsevier BV",
number = "2",

}

RIS

TY - JOUR

T1 - Spatial and temporal expression patterns of chitinase genes in developing zebrafish embryos

AU - Koch, Bjørn Erik Vind

AU - Stougaard, Jens

AU - Spaink, Herman P

N1 - Copyright © 2014. Published by Elsevier B.V.

PY - 2014/3

Y1 - 2014/3

N2 - Chitinases and chitinase like proteins play an important role in mammalian immunity and functions in early zebrafish development have been suggested. Here we report identification of six zebrafish chitinases and chitinase like proteins (called CHIA.1-6) belonging to the glycoside hydrolase family 18, and determine their spatial and temporal expression at 10 stages of zebrafish development. CHIA.4 is highly maternally expressed and it is expressed 100 fold above any other CHIA gene at zygote through to blastula stage. Later, after the maternal to zygotic transition, CHIA.4 expression decreases to the same level as CHIA.5 and CHIA.6. Subsequently, CHIA.1, CHIA.2, CHIA.3 and CHIA.4, CHIA.5, CHIA.6 each follow distinct paths in terms of expression levels. Until 4days post fertilization the spatial expression patterns of all six CHIA genes overlap extensively, with expression detected predominantly in vascular, ocular and intestinal tissues. At 5days post fertilization CHIA.1, CHIA.2 and CHIA.3 are expressed almost exclusively in the stomach, whereas CHIA.4, CHIA.5 and CHIA.6 are also prominently expressed in the liver. These different expression patterns may contribute to the establishment of a basis, on which functional analysis in older larvae may be founded.

AB - Chitinases and chitinase like proteins play an important role in mammalian immunity and functions in early zebrafish development have been suggested. Here we report identification of six zebrafish chitinases and chitinase like proteins (called CHIA.1-6) belonging to the glycoside hydrolase family 18, and determine their spatial and temporal expression at 10 stages of zebrafish development. CHIA.4 is highly maternally expressed and it is expressed 100 fold above any other CHIA gene at zygote through to blastula stage. Later, after the maternal to zygotic transition, CHIA.4 expression decreases to the same level as CHIA.5 and CHIA.6. Subsequently, CHIA.1, CHIA.2, CHIA.3 and CHIA.4, CHIA.5, CHIA.6 each follow distinct paths in terms of expression levels. Until 4days post fertilization the spatial expression patterns of all six CHIA genes overlap extensively, with expression detected predominantly in vascular, ocular and intestinal tissues. At 5days post fertilization CHIA.1, CHIA.2 and CHIA.3 are expressed almost exclusively in the stomach, whereas CHIA.4, CHIA.5 and CHIA.6 are also prominently expressed in the liver. These different expression patterns may contribute to the establishment of a basis, on which functional analysis in older larvae may be founded.

U2 - 10.1016/j.gep.2014.01.001

DO - 10.1016/j.gep.2014.01.001

M3 - Journal article

C2 - 24418193

VL - 14

SP - 69

EP - 77

JO - Gene Expression Patterns

JF - Gene Expression Patterns

SN - 1567-133X

IS - 2

ER -