Solvent‐Free Photobiocatalytic Hydroxylation of Cyclohexane

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Standard

Solvent‐Free Photobiocatalytic Hydroxylation of Cyclohexane. / Hobisch, Markus; van Schie, Morten Martinus Cornelis Harald; Kim, Jinhyun; Andersen, Kasper Røjkjær; Alcalde, Miguel; Kourist, Robert ; Park, Chan Beum; Hollmann, Frank; Kara, Selin.

I: ChemCatChem, Bind 12, Nr. 16, 08.2020, s. 4009-4013.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Hobisch, M, van Schie, MMCH, Kim, J, Andersen, KR, Alcalde, M, Kourist, R, Park, CB, Hollmann, F & Kara, S 2020, 'Solvent‐Free Photobiocatalytic Hydroxylation of Cyclohexane', ChemCatChem, bind 12, nr. 16, s. 4009-4013. https://doi.org/10.1002/cctc.202000512

APA

Hobisch, M., van Schie, M. M. C. H., Kim, J., Andersen, K. R., Alcalde, M., Kourist, R., Park, C. B., Hollmann, F., & Kara, S. (2020). Solvent‐Free Photobiocatalytic Hydroxylation of Cyclohexane. ChemCatChem, 12(16), 4009-4013. https://doi.org/10.1002/cctc.202000512

CBE

Hobisch M, van Schie MMCH, Kim J, Andersen KR, Alcalde M, Kourist R, Park CB, Hollmann F, Kara S. 2020. Solvent‐Free Photobiocatalytic Hydroxylation of Cyclohexane. ChemCatChem. 12(16):4009-4013. https://doi.org/10.1002/cctc.202000512

MLA

Vancouver

Hobisch M, van Schie MMCH, Kim J, Andersen KR, Alcalde M, Kourist R o.a. Solvent‐Free Photobiocatalytic Hydroxylation of Cyclohexane. ChemCatChem. 2020 aug;12(16):4009-4013. https://doi.org/10.1002/cctc.202000512

Author

Hobisch, Markus ; van Schie, Morten Martinus Cornelis Harald ; Kim, Jinhyun ; Andersen, Kasper Røjkjær ; Alcalde, Miguel ; Kourist, Robert ; Park, Chan Beum ; Hollmann, Frank ; Kara, Selin. / Solvent‐Free Photobiocatalytic Hydroxylation of Cyclohexane. I: ChemCatChem. 2020 ; Bind 12, Nr. 16. s. 4009-4013.

Bibtex

@article{f5fec18a36524290998bae1bebf91ce4,
title = "Solvent‐Free Photobiocatalytic Hydroxylation of Cyclohexane",
abstract = "The use of neat reaction media, that is the avoidance of additional solvents, is the simplest and the most efficient approach to follow in biocatalysis. Here, we show that unspecific peroxygenase from Agrocybe aegerita (AaeUPO) can hydroxylate the neat model substrate cyclohexane. H 2O 2 was photocatalytically generated in situ by nitrogen-doped carbon nanodots (N−CNDs) and UV LED illumination. AaeUPO entrapment in alginate beads increased enzyme stability and facilitated the reaction in neat cyclohexane. N−CNDs absorption in beads containing AaeUPO created a 2-in-1 heterogeneous photobiocatalyst that was active for up to seven days under reaction conditions and produced cyclohexanol, 2.5 mM. To increase productivity, the bead size and the photocatalyst-to-enzyme ratio have been identified as promising targets for optimisation. ",
keywords = "Photobiocatalysis, carbon nanodots, hydroxylation, non-conventional media, organic media",
author = "Markus Hobisch and {van Schie}, {Morten Martinus Cornelis Harald} and Jinhyun Kim and Andersen, {Kasper R{\o}jkj{\ae}r} and Miguel Alcalde and Robert Kourist and Park, {Chan Beum} and Frank Hollmann and Selin Kara",
year = "2020",
month = aug,
doi = "10.1002/cctc.202000512",
language = "English",
volume = "12",
pages = "4009--4013",
journal = "ChemCatChem",
issn = "1867-3880",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "16",

}

RIS

TY - JOUR

T1 - Solvent‐Free Photobiocatalytic Hydroxylation of Cyclohexane

AU - Hobisch, Markus

AU - van Schie, Morten Martinus Cornelis Harald

AU - Kim, Jinhyun

AU - Andersen, Kasper Røjkjær

AU - Alcalde, Miguel

AU - Kourist, Robert

AU - Park, Chan Beum

AU - Hollmann, Frank

AU - Kara, Selin

PY - 2020/8

Y1 - 2020/8

N2 - The use of neat reaction media, that is the avoidance of additional solvents, is the simplest and the most efficient approach to follow in biocatalysis. Here, we show that unspecific peroxygenase from Agrocybe aegerita (AaeUPO) can hydroxylate the neat model substrate cyclohexane. H 2O 2 was photocatalytically generated in situ by nitrogen-doped carbon nanodots (N−CNDs) and UV LED illumination. AaeUPO entrapment in alginate beads increased enzyme stability and facilitated the reaction in neat cyclohexane. N−CNDs absorption in beads containing AaeUPO created a 2-in-1 heterogeneous photobiocatalyst that was active for up to seven days under reaction conditions and produced cyclohexanol, 2.5 mM. To increase productivity, the bead size and the photocatalyst-to-enzyme ratio have been identified as promising targets for optimisation.

AB - The use of neat reaction media, that is the avoidance of additional solvents, is the simplest and the most efficient approach to follow in biocatalysis. Here, we show that unspecific peroxygenase from Agrocybe aegerita (AaeUPO) can hydroxylate the neat model substrate cyclohexane. H 2O 2 was photocatalytically generated in situ by nitrogen-doped carbon nanodots (N−CNDs) and UV LED illumination. AaeUPO entrapment in alginate beads increased enzyme stability and facilitated the reaction in neat cyclohexane. N−CNDs absorption in beads containing AaeUPO created a 2-in-1 heterogeneous photobiocatalyst that was active for up to seven days under reaction conditions and produced cyclohexanol, 2.5 mM. To increase productivity, the bead size and the photocatalyst-to-enzyme ratio have been identified as promising targets for optimisation.

KW - Photobiocatalysis

KW - carbon nanodots

KW - hydroxylation

KW - non-conventional media

KW - organic media

U2 - 10.1002/cctc.202000512

DO - 10.1002/cctc.202000512

M3 - Journal article

VL - 12

SP - 4009

EP - 4013

JO - ChemCatChem

JF - ChemCatChem

SN - 1867-3880

IS - 16

ER -