Aarhus Universitets segl

English

Du er her: AU » Om AU » Organisation » Small Organic Catalase Mimic Encapsulated in Micellar Art...

Om AU

Small Organic Catalase Mimic Encapsulated in Micellar Artificial Organelles as Reactive Oxygen Species Scavengers

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

DOI

  • Carina Aileen Ade
    • ,
  • Edit Brodszkij
    • ,
  • Bo Thingholm
    • ,
  • Noga Gal
    • ,
  • Fabian Robert Itel
    • ,
  • Essi Marika Taipaleenmäki
    • ,
  • Philipp Schattling
    • ,
  • Brigitte Maria Stadler
Biocatalytic intracellular active nanoreactors (artificial organelles) aim to support their host cells. Here, we report the first successful micelle-based artificial organelles containing a salen–manganese complex (EUK) as catalase mimic with intracellular activity in HepG2 cells to act as reactive oxygen species (ROS) scavengers. Four different EUKs were synthesized and compared in their ability to convert hydrogen peroxide to water and oxygen as free compounds and when encapsulated into micelles assembled from the amphiphilic block copolymer poly(cholesteryl methacrylate)-block-poly(2-(dimethylamino)ethyl methacrylate). An EUK candidate with an asymmetric substitution of chemical groups at the ortho and the meta position (EUK-B) was identified as lead candidate. HepG2 cells continued proliferating when preincubated with low concentrations of EUK-B-containing micelles (MB). Importantly, HepG2 cells equipped with MB showed improved viability compared to the controls when stressed with paraquat, a compound that induces ROS generation. The intracellular activity of MB was supported by lower amounts of intracellular detectable ROS. This first report on the combination of artificial enzymes and artificial organelles further extends the opportunities in therapeutic cell mimicry.
OriginalsprogEngelsk
TidsskriftACS Applied Polymer Materials
Vol/bind1
Nummer6
Sider (fra-til)1532-1539
Antal sider8
ISSN2637-6105
DOI
StatusUdgivet - jun. 2019

Se relationer på Aarhus Universitet Citationsformater

ID: 162995418